Cargando…

Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p

Circular RNAs (circRNAs) are a new class of single-stranded RNAs that form a continuous loop with crucial role in regulation of gene expression. Because their circular conformation conforms numerous properties, circRNAs have been investigated recently to demonstrate their important role in the devel...

Descripción completa

Detalles Bibliográficos
Autores principales: Liao, Wenyan, He, Jun, Disoma, Cyrollah, Hu, Yi, Li, Junhua, Chen, Guodong, Sheng, Ying, Cai, Xinyu, Li, Chuanfu, Cheng, Kai, Yang, Chunfen, Qin, Yongxi, Han, Dong, Wen, Wu, Ding, Chengming, Li, Mujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874083/
https://www.ncbi.nlm.nih.gov/pubmed/33585208
http://dx.doi.org/10.3389/fonc.2020.590627
_version_ 1783649515548442624
author Liao, Wenyan
He, Jun
Disoma, Cyrollah
Hu, Yi
Li, Junhua
Chen, Guodong
Sheng, Ying
Cai, Xinyu
Li, Chuanfu
Cheng, Kai
Yang, Chunfen
Qin, Yongxi
Han, Dong
Wen, Wu
Ding, Chengming
Li, Mujun
author_facet Liao, Wenyan
He, Jun
Disoma, Cyrollah
Hu, Yi
Li, Junhua
Chen, Guodong
Sheng, Ying
Cai, Xinyu
Li, Chuanfu
Cheng, Kai
Yang, Chunfen
Qin, Yongxi
Han, Dong
Wen, Wu
Ding, Chengming
Li, Mujun
author_sort Liao, Wenyan
collection PubMed
description Circular RNAs (circRNAs) are a new class of single-stranded RNAs that form a continuous loop with crucial role in regulation of gene expression. Because their circular conformation conforms numerous properties, circRNAs have been investigated recently to demonstrate their important role in the development and progression of various cancers. However, the function of circRNAs and their regulatory outcomes in cervical cancer (CC) have rarely been explored. In this study, the role and molecular mechanism of hsa_circ_0107593 in cervical cancer are demonstrated. Quantitative polymerase chain reaction (qRT-PCR) was used to determine the expression of hsa_circ_0107593 and three miRNAs (hsa-miR-20a-5p, 93-5p, and 106b-5p) in paired CC tissues (tumor tissue vs. adjacent normal cervical tissue), CC cell lines, and human normal cervical epithelial immortalized cell line. A series of functional experiments were conducted to assess the function of hsa_circ_0107593 in CC development. The Receiver Operating Characteristic (ROC) curve was plotted to estimate the diagnostic value of hsa_circ_0107593 in CC. The dual-luciferase reporter assay was used to explore the interaction between hsa_circ_0107593 and hsa-miR-20a-5p/93-5p/106b-5p. Bioinformatic analysis was conducted to predict the target mRNAs, pathways, and functional enrichment. The results revealed that hsa_circ_0107593 has low expression in CC tissues and CC cell lines. Moreover, negative correlations of hsa_circ_0107593 expression were found against tumor diameter, FIGO stage, and myometrial invasion. Also, hsa_circ_0107593 impedes CC cell proliferation, migration, and invasion. Based on ROC curve analysis, hsa_circ_0107593 could serve as a diagnostic biomarker. Its low expression may indicate increased patient’s risk to developing cervical cancer. Mechanistically, hsa_circ_0107593 serves as a sponge of hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p. Collectively, our study implies that hsa_circ_0107593 has tumor-suppressing activity in CC by physically binding with hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p.
format Online
Article
Text
id pubmed-7874083
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78740832021-02-11 Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p Liao, Wenyan He, Jun Disoma, Cyrollah Hu, Yi Li, Junhua Chen, Guodong Sheng, Ying Cai, Xinyu Li, Chuanfu Cheng, Kai Yang, Chunfen Qin, Yongxi Han, Dong Wen, Wu Ding, Chengming Li, Mujun Front Oncol Oncology Circular RNAs (circRNAs) are a new class of single-stranded RNAs that form a continuous loop with crucial role in regulation of gene expression. Because their circular conformation conforms numerous properties, circRNAs have been investigated recently to demonstrate their important role in the development and progression of various cancers. However, the function of circRNAs and their regulatory outcomes in cervical cancer (CC) have rarely been explored. In this study, the role and molecular mechanism of hsa_circ_0107593 in cervical cancer are demonstrated. Quantitative polymerase chain reaction (qRT-PCR) was used to determine the expression of hsa_circ_0107593 and three miRNAs (hsa-miR-20a-5p, 93-5p, and 106b-5p) in paired CC tissues (tumor tissue vs. adjacent normal cervical tissue), CC cell lines, and human normal cervical epithelial immortalized cell line. A series of functional experiments were conducted to assess the function of hsa_circ_0107593 in CC development. The Receiver Operating Characteristic (ROC) curve was plotted to estimate the diagnostic value of hsa_circ_0107593 in CC. The dual-luciferase reporter assay was used to explore the interaction between hsa_circ_0107593 and hsa-miR-20a-5p/93-5p/106b-5p. Bioinformatic analysis was conducted to predict the target mRNAs, pathways, and functional enrichment. The results revealed that hsa_circ_0107593 has low expression in CC tissues and CC cell lines. Moreover, negative correlations of hsa_circ_0107593 expression were found against tumor diameter, FIGO stage, and myometrial invasion. Also, hsa_circ_0107593 impedes CC cell proliferation, migration, and invasion. Based on ROC curve analysis, hsa_circ_0107593 could serve as a diagnostic biomarker. Its low expression may indicate increased patient’s risk to developing cervical cancer. Mechanistically, hsa_circ_0107593 serves as a sponge of hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p. Collectively, our study implies that hsa_circ_0107593 has tumor-suppressing activity in CC by physically binding with hsa-miR-20a-5p, hsa-miR-93-5p, and hsa-miR-106b-5p. Frontiers Media S.A. 2021-01-15 /pmc/articles/PMC7874083/ /pubmed/33585208 http://dx.doi.org/10.3389/fonc.2020.590627 Text en Copyright © 2021 Liao, He, Disoma, Hu, Li, Chen, Sheng, Cai, Li, Cheng, Yang, Qin, Han, Wen, Ding and Li http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Liao, Wenyan
He, Jun
Disoma, Cyrollah
Hu, Yi
Li, Junhua
Chen, Guodong
Sheng, Ying
Cai, Xinyu
Li, Chuanfu
Cheng, Kai
Yang, Chunfen
Qin, Yongxi
Han, Dong
Wen, Wu
Ding, Chengming
Li, Mujun
Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title_full Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title_fullStr Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title_full_unstemmed Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title_short Hsa_circ_0107593 Suppresses the Progression of Cervical Cancer via Sponging hsa-miR-20a-5p/93-5p/106b-5p
title_sort hsa_circ_0107593 suppresses the progression of cervical cancer via sponging hsa-mir-20a-5p/93-5p/106b-5p
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874083/
https://www.ncbi.nlm.nih.gov/pubmed/33585208
http://dx.doi.org/10.3389/fonc.2020.590627
work_keys_str_mv AT liaowenyan hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT hejun hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT disomacyrollah hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT huyi hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT lijunhua hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT chenguodong hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT shengying hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT caixinyu hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT lichuanfu hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT chengkai hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT yangchunfen hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT qinyongxi hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT handong hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT wenwu hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT dingchengming hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p
AT limujun hsacirc0107593suppressestheprogressionofcervicalcancerviasponginghsamir20a5p935p106b5p