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Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates

Bacterial resistance to known antibiotics comprises a serious threat to public health. Propagation of multidrug-resistant pathogenic strains is a reason for undertaking a search for new therapeutic strategies, based on newly developed chemical compounds and the agents present in nature. Moreover, an...

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Autores principales: Nowicki, Dariusz, Krause, Klaudyna, Szamborska, Patrycja, Żukowska, Adrianna, Cech, Grzegorz M., Szalewska-Pałasz, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874123/
https://www.ncbi.nlm.nih.gov/pubmed/33584562
http://dx.doi.org/10.3389/fmicb.2020.591802
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author Nowicki, Dariusz
Krause, Klaudyna
Szamborska, Patrycja
Żukowska, Adrianna
Cech, Grzegorz M.
Szalewska-Pałasz, Agnieszka
author_facet Nowicki, Dariusz
Krause, Klaudyna
Szamborska, Patrycja
Żukowska, Adrianna
Cech, Grzegorz M.
Szalewska-Pałasz, Agnieszka
author_sort Nowicki, Dariusz
collection PubMed
description Bacterial resistance to known antibiotics comprises a serious threat to public health. Propagation of multidrug-resistant pathogenic strains is a reason for undertaking a search for new therapeutic strategies, based on newly developed chemical compounds and the agents present in nature. Moreover, antibiotic treatment of infections caused by enterotoxin toxin-bearing strain—enterohemorrhagic Escherichia coli (EHEC) is considered hazardous and controversial due to the possibility of induction of bacteriophage-encoded toxin production by the antibiotic-mediated stress. The important source of potentially beneficial compounds are secondary plant metabolites, isothiocyanates (ITC), and phytoncides from the Brassicaceae family. We reported previously that sulforaphane and phenethyl isothiocyanate, already known for their chemopreventive and anticancer features, exhibit significant antibacterial effects against various pathogenic bacteria. The mechanism of their action is based on the induction of the stringent response and accumulation of its alarmones, the guanosine penta- and tetraphosphate. In this process, the amino acid starvation path is employed via the RelA protein, however, the precise mechanism of amino acid limitation in the presence of ITCs is yet unknown. In this work, we asked whether ITCs could act synergistically with each other to increase the antibacterial effect. A set of aliphatic ITCs, such as iberin, iberverin, alyssin, erucin, sulforaphen, erysolin, and cheirolin was tested in combination with sulforaphane against E. coli. Our experiments show that all tested ITCs exhibit strong antimicrobial effect individually, and this effect involves the stringent response caused by induction of the amino acid starvation. Interestingly, excess of specific amino acids reversed the antimicrobial effects of ITCs, where the common amino acid for all tested compounds was glycine. The synergistic action observed for iberin, iberverin, and alyssin also led to accumulation of (p)ppGpp, and the minimal inhibitory concentration necessary for the antibacterial effect was four- to eightfold lower than for individual ITCs. Moreover, the unique mode of ITC action is responsible for inhibition of prophage induction and toxin production, in addition to growth inhibition of EHEC strains. Thus, the antimicrobial effect of plant secondary metabolites by the stringent response induction could be employed in potential therapeutic strategies.
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spelling pubmed-78741232021-02-11 Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates Nowicki, Dariusz Krause, Klaudyna Szamborska, Patrycja Żukowska, Adrianna Cech, Grzegorz M. Szalewska-Pałasz, Agnieszka Front Microbiol Microbiology Bacterial resistance to known antibiotics comprises a serious threat to public health. Propagation of multidrug-resistant pathogenic strains is a reason for undertaking a search for new therapeutic strategies, based on newly developed chemical compounds and the agents present in nature. Moreover, antibiotic treatment of infections caused by enterotoxin toxin-bearing strain—enterohemorrhagic Escherichia coli (EHEC) is considered hazardous and controversial due to the possibility of induction of bacteriophage-encoded toxin production by the antibiotic-mediated stress. The important source of potentially beneficial compounds are secondary plant metabolites, isothiocyanates (ITC), and phytoncides from the Brassicaceae family. We reported previously that sulforaphane and phenethyl isothiocyanate, already known for their chemopreventive and anticancer features, exhibit significant antibacterial effects against various pathogenic bacteria. The mechanism of their action is based on the induction of the stringent response and accumulation of its alarmones, the guanosine penta- and tetraphosphate. In this process, the amino acid starvation path is employed via the RelA protein, however, the precise mechanism of amino acid limitation in the presence of ITCs is yet unknown. In this work, we asked whether ITCs could act synergistically with each other to increase the antibacterial effect. A set of aliphatic ITCs, such as iberin, iberverin, alyssin, erucin, sulforaphen, erysolin, and cheirolin was tested in combination with sulforaphane against E. coli. Our experiments show that all tested ITCs exhibit strong antimicrobial effect individually, and this effect involves the stringent response caused by induction of the amino acid starvation. Interestingly, excess of specific amino acids reversed the antimicrobial effects of ITCs, where the common amino acid for all tested compounds was glycine. The synergistic action observed for iberin, iberverin, and alyssin also led to accumulation of (p)ppGpp, and the minimal inhibitory concentration necessary for the antibacterial effect was four- to eightfold lower than for individual ITCs. Moreover, the unique mode of ITC action is responsible for inhibition of prophage induction and toxin production, in addition to growth inhibition of EHEC strains. Thus, the antimicrobial effect of plant secondary metabolites by the stringent response induction could be employed in potential therapeutic strategies. Frontiers Media S.A. 2021-01-14 /pmc/articles/PMC7874123/ /pubmed/33584562 http://dx.doi.org/10.3389/fmicb.2020.591802 Text en Copyright © 2021 Nowicki, Krause, Szamborska, Żukowska, Cech and Szalewska-Pałasz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nowicki, Dariusz
Krause, Klaudyna
Szamborska, Patrycja
Żukowska, Adrianna
Cech, Grzegorz M.
Szalewska-Pałasz, Agnieszka
Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title_full Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title_fullStr Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title_full_unstemmed Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title_short Induction of the Stringent Response Underlies the Antimicrobial Action of Aliphatic Isothiocyanates
title_sort induction of the stringent response underlies the antimicrobial action of aliphatic isothiocyanates
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874123/
https://www.ncbi.nlm.nih.gov/pubmed/33584562
http://dx.doi.org/10.3389/fmicb.2020.591802
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