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Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces

Shiga toxin-producing Escherichia coli (STEC) causes foodborne outbreaks that can lead to complications such as hemolytic uremic syndrome. Their main reservoir is cattle, and ground beef has been frequently associated with disease and outbreaks. In this study, we attempted to understand the genetic...

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Autores principales: Gutiérrez, Sebastián, Díaz, Leonela, Reyes-Jara, Angélica, Yang, Xun, Meng, Jianghong, González-Escalona, Narjol, Toro, Magaly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874142/
https://www.ncbi.nlm.nih.gov/pubmed/33584592
http://dx.doi.org/10.3389/fmicb.2020.622663
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author Gutiérrez, Sebastián
Díaz, Leonela
Reyes-Jara, Angélica
Yang, Xun
Meng, Jianghong
González-Escalona, Narjol
Toro, Magaly
author_facet Gutiérrez, Sebastián
Díaz, Leonela
Reyes-Jara, Angélica
Yang, Xun
Meng, Jianghong
González-Escalona, Narjol
Toro, Magaly
author_sort Gutiérrez, Sebastián
collection PubMed
description Shiga toxin-producing Escherichia coli (STEC) causes foodborne outbreaks that can lead to complications such as hemolytic uremic syndrome. Their main reservoir is cattle, and ground beef has been frequently associated with disease and outbreaks. In this study, we attempted to understand the genetic relationship among STEC isolated in Chile from different sources, their relationship to STEC from the rest of the world, and to identify molecular markers of Chilean STEC. We sequenced 62 STEC isolated in Chile using MiSeq Illumina. In silico typing was determined using tools of the Center Genomic Epidemiology, Denmark University (CGE/DTU). Genomes of our local STEC collection were compared with 113 STEC isolated worldwide through a core genome MLST (cgMLST) approach, and we also searched for distinct genes to be used as molecular markers of Chilean isolates. Genomes in our local collection were grouped based on serogroup and sequence type, and clusters were formed within local STEC. In the worldwide STEC analysis, Chilean STEC did not cluster with genomes of the rest of the world suggesting that they are not phylogenetically related to previously described STEC. The pangenome of our STEC collection was 11,650 genes, but we did not identify distinct molecular markers of local STEC. Our results showed that there may be local emerging STEC with unique features, nevertheless, no molecular markers were detected. Therefore, there might be elements such as a syntenic organization that might explain differential clustering detected between local and worldwide STEC.
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spelling pubmed-78741422021-02-11 Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces Gutiérrez, Sebastián Díaz, Leonela Reyes-Jara, Angélica Yang, Xun Meng, Jianghong González-Escalona, Narjol Toro, Magaly Front Microbiol Microbiology Shiga toxin-producing Escherichia coli (STEC) causes foodborne outbreaks that can lead to complications such as hemolytic uremic syndrome. Their main reservoir is cattle, and ground beef has been frequently associated with disease and outbreaks. In this study, we attempted to understand the genetic relationship among STEC isolated in Chile from different sources, their relationship to STEC from the rest of the world, and to identify molecular markers of Chilean STEC. We sequenced 62 STEC isolated in Chile using MiSeq Illumina. In silico typing was determined using tools of the Center Genomic Epidemiology, Denmark University (CGE/DTU). Genomes of our local STEC collection were compared with 113 STEC isolated worldwide through a core genome MLST (cgMLST) approach, and we also searched for distinct genes to be used as molecular markers of Chilean isolates. Genomes in our local collection were grouped based on serogroup and sequence type, and clusters were formed within local STEC. In the worldwide STEC analysis, Chilean STEC did not cluster with genomes of the rest of the world suggesting that they are not phylogenetically related to previously described STEC. The pangenome of our STEC collection was 11,650 genes, but we did not identify distinct molecular markers of local STEC. Our results showed that there may be local emerging STEC with unique features, nevertheless, no molecular markers were detected. Therefore, there might be elements such as a syntenic organization that might explain differential clustering detected between local and worldwide STEC. Frontiers Media S.A. 2021-01-18 /pmc/articles/PMC7874142/ /pubmed/33584592 http://dx.doi.org/10.3389/fmicb.2020.622663 Text en Copyright © 2021 Gutiérrez, Díaz, Reyes-Jara, Yang, Meng, González-Escalona and Toro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gutiérrez, Sebastián
Díaz, Leonela
Reyes-Jara, Angélica
Yang, Xun
Meng, Jianghong
González-Escalona, Narjol
Toro, Magaly
Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title_full Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title_fullStr Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title_full_unstemmed Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title_short Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces
title_sort whole-genome phylogenetic analysis reveals a wide diversity of non-o157 stec isolated from ground beef and cattle feces
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874142/
https://www.ncbi.nlm.nih.gov/pubmed/33584592
http://dx.doi.org/10.3389/fmicb.2020.622663
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