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The testis-specifically expressed gene Trim69 is not essential for fertility in mice
Protein ubiquitination is essential for diverse cellular functions including spermatogenesis. The tripartite motif (TRIM) family proteins, most of which have E3 ubiquitin ligase activity, are highly conserved in mammals. They are involved in important cellular processes such as embryonic development...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Editorial Department of Journal of Biomedical Research
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874274/ https://www.ncbi.nlm.nih.gov/pubmed/33273151 http://dx.doi.org/10.7555/JBR.34.20200069 |
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author | He, Xi Xie, Wenxiu Li, Huiling Cui, Yiqiang Wang, Ya Guo, Xuejiang Sha, Jiahao |
author_facet | He, Xi Xie, Wenxiu Li, Huiling Cui, Yiqiang Wang, Ya Guo, Xuejiang Sha, Jiahao |
author_sort | He, Xi |
collection | PubMed |
description | Protein ubiquitination is essential for diverse cellular functions including spermatogenesis. The tripartite motif (TRIM) family proteins, most of which have E3 ubiquitin ligase activity, are highly conserved in mammals. They are involved in important cellular processes such as embryonic development, immunity, and fertility. Our previous studies indicated that Trim69, a testis-specific expressed TRIM family gene, potentially participates in the spermatogenesis by mediating testicular cells apoptosis. In this study, we investigated the biological functions of Trim69 in male mice by established Trim69 knockout mice with CRISPR/Cas9 genomic editing technology. Here, we reported that the male Trim69 knockout mice had normal fertility. The adult knockout mice have shown that the appearance of testes, testis/body weight ratios, testicular histomorphology, and the number and quality of sperm were consistent with wild-type mice. These results indicated that the E3 ubiquitin ligase protein Trim69 was not essential for male mouse fertility, and it might be compensated by other TRIM family members such as Trim58 in Trim69-deficiency testis. This study would help to elucidate the functions of tripartite motif protein family and the regulation of spermatogenesis. |
format | Online Article Text |
id | pubmed-7874274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Editorial Department of Journal of Biomedical Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-78742742021-02-12 The testis-specifically expressed gene Trim69 is not essential for fertility in mice He, Xi Xie, Wenxiu Li, Huiling Cui, Yiqiang Wang, Ya Guo, Xuejiang Sha, Jiahao J Biomed Res Original Article Protein ubiquitination is essential for diverse cellular functions including spermatogenesis. The tripartite motif (TRIM) family proteins, most of which have E3 ubiquitin ligase activity, are highly conserved in mammals. They are involved in important cellular processes such as embryonic development, immunity, and fertility. Our previous studies indicated that Trim69, a testis-specific expressed TRIM family gene, potentially participates in the spermatogenesis by mediating testicular cells apoptosis. In this study, we investigated the biological functions of Trim69 in male mice by established Trim69 knockout mice with CRISPR/Cas9 genomic editing technology. Here, we reported that the male Trim69 knockout mice had normal fertility. The adult knockout mice have shown that the appearance of testes, testis/body weight ratios, testicular histomorphology, and the number and quality of sperm were consistent with wild-type mice. These results indicated that the E3 ubiquitin ligase protein Trim69 was not essential for male mouse fertility, and it might be compensated by other TRIM family members such as Trim58 in Trim69-deficiency testis. This study would help to elucidate the functions of tripartite motif protein family and the regulation of spermatogenesis. Editorial Department of Journal of Biomedical Research 2021-01 2020-08-21 /pmc/articles/PMC7874274/ /pubmed/33273151 http://dx.doi.org/10.7555/JBR.34.20200069 Text en Copyright and License information: Journal of Biomedical Research, CAS Springer-Verlag Berlin Heidelberg 2021 http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Original Article He, Xi Xie, Wenxiu Li, Huiling Cui, Yiqiang Wang, Ya Guo, Xuejiang Sha, Jiahao The testis-specifically expressed gene Trim69 is not essential for fertility in mice |
title | The testis-specifically expressed gene Trim69 is not essential for fertility in mice
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title_full | The testis-specifically expressed gene Trim69 is not essential for fertility in mice
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title_fullStr | The testis-specifically expressed gene Trim69 is not essential for fertility in mice
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title_full_unstemmed | The testis-specifically expressed gene Trim69 is not essential for fertility in mice
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title_short | The testis-specifically expressed gene Trim69 is not essential for fertility in mice
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title_sort | testis-specifically expressed gene trim69 is not essential for fertility in mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874274/ https://www.ncbi.nlm.nih.gov/pubmed/33273151 http://dx.doi.org/10.7555/JBR.34.20200069 |
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