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Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression
This study investigates the transcriptome response of meniscus fibrochondrocytes (MFCs) to the low oxygen and mechanical loading signals experienced in the knee joint using a model system. We hypothesized that short term exposure to the combined treatment would promote a matrix-forming phenotype sup...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874349/ https://www.ncbi.nlm.nih.gov/pubmed/33613959 http://dx.doi.org/10.1177/2041731421990842 |
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author | Szojka, Alexander RA Marqueti, Rita de Cássia Li, David Xinzheyang Molter, Clayton W Liang, Yan Kunze, Melanie Mulet-Sierra, Aillette Jomha, Nadr M Adesida, Adetola B |
author_facet | Szojka, Alexander RA Marqueti, Rita de Cássia Li, David Xinzheyang Molter, Clayton W Liang, Yan Kunze, Melanie Mulet-Sierra, Aillette Jomha, Nadr M Adesida, Adetola B |
author_sort | Szojka, Alexander RA |
collection | PubMed |
description | This study investigates the transcriptome response of meniscus fibrochondrocytes (MFCs) to the low oxygen and mechanical loading signals experienced in the knee joint using a model system. We hypothesized that short term exposure to the combined treatment would promote a matrix-forming phenotype supportive of inner meniscus tissue formation. Human MFCs on a collagen scaffold were stimulated to form fibrocartilage over 6 weeks under normoxic (NRX, 20% O(2)) conditions with supplemented TGF-β3. Tissues experienced a delayed 24h hypoxia treatment (HYP, 3% O(2)) and then 5 min of dynamic compression (DC) between 30 and 40% strain. Delayed HYP induced an anabolic and anti-catabolic expression profile for hyaline cartilage matrix markers, while DC induced an inflammatory matrix remodeling response along with upregulation of both SOX9 and COL1A1. There were 41 genes regulated by both HYP and DC. Overall, the combined treatment supported a unique gene expression profile favouring the hyaline cartilage aspect of inner meniscus matrix and matrix remodeling. |
format | Online Article Text |
id | pubmed-7874349 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-78743492021-02-19 Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression Szojka, Alexander RA Marqueti, Rita de Cássia Li, David Xinzheyang Molter, Clayton W Liang, Yan Kunze, Melanie Mulet-Sierra, Aillette Jomha, Nadr M Adesida, Adetola B J Tissue Eng Original Article This study investigates the transcriptome response of meniscus fibrochondrocytes (MFCs) to the low oxygen and mechanical loading signals experienced in the knee joint using a model system. We hypothesized that short term exposure to the combined treatment would promote a matrix-forming phenotype supportive of inner meniscus tissue formation. Human MFCs on a collagen scaffold were stimulated to form fibrocartilage over 6 weeks under normoxic (NRX, 20% O(2)) conditions with supplemented TGF-β3. Tissues experienced a delayed 24h hypoxia treatment (HYP, 3% O(2)) and then 5 min of dynamic compression (DC) between 30 and 40% strain. Delayed HYP induced an anabolic and anti-catabolic expression profile for hyaline cartilage matrix markers, while DC induced an inflammatory matrix remodeling response along with upregulation of both SOX9 and COL1A1. There were 41 genes regulated by both HYP and DC. Overall, the combined treatment supported a unique gene expression profile favouring the hyaline cartilage aspect of inner meniscus matrix and matrix remodeling. SAGE Publications 2021-02-06 /pmc/articles/PMC7874349/ /pubmed/33613959 http://dx.doi.org/10.1177/2041731421990842 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Szojka, Alexander RA Marqueti, Rita de Cássia Li, David Xinzheyang Molter, Clayton W Liang, Yan Kunze, Melanie Mulet-Sierra, Aillette Jomha, Nadr M Adesida, Adetola B Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title | Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title_full | Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title_fullStr | Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title_full_unstemmed | Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title_short | Human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
title_sort | human engineered meniscus transcriptome after short-term combined hypoxia and dynamic compression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874349/ https://www.ncbi.nlm.nih.gov/pubmed/33613959 http://dx.doi.org/10.1177/2041731421990842 |
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