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Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola

Biocontrol is a cost-effective and environmentally friendly technique used in agricultural production. We isolated and screened a bacterial strain from the soils of a peach orchard with high yield. Using biochemical and physiological analysis as well as phylogenetic sequencing data, we identified a...

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Autores principales: Yuan, Xue, Hou, Xu, Chang, Haotian, Yang, Rui, Wang, Fang, Liu, Yueping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874823/
https://www.ncbi.nlm.nih.gov/pubmed/33817176
http://dx.doi.org/10.1515/biol-2019-0046
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author Yuan, Xue
Hou, Xu
Chang, Haotian
Yang, Rui
Wang, Fang
Liu, Yueping
author_facet Yuan, Xue
Hou, Xu
Chang, Haotian
Yang, Rui
Wang, Fang
Liu, Yueping
author_sort Yuan, Xue
collection PubMed
description Biocontrol is a cost-effective and environmentally friendly technique used in agricultural production. We isolated and screened a bacterial strain from the soils of a peach orchard with high yield. Using biochemical and physiological analysis as well as phylogenetic sequencing data, we identified a strain of Bacillus methylotrophicus, strain XJ-C. The results of our screening trials showed that XJ-C was able to suppress M. fructicola at an inhibition rate of 81.57%. Following the application of a 1×10(9) CFU/mL XJ-C strain suspension to the fruits, leaves, and shoots of peach trees infected with M. fructicola, the inhibition rate reached 64.31%, 97.34%, and 64.28%, respectively. Using OM and SEM, we observed that, under the inhibition of strain XJ-C, M. fructicola mycelium and spores were abnormally shaped. Under TEM, cell walls were transparent, organelles had disappeared, and the intracellular vacuole was deformed. Thus, XJ-C has the potential to be used in biocontrol.
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spelling pubmed-78748232021-04-01 Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola Yuan, Xue Hou, Xu Chang, Haotian Yang, Rui Wang, Fang Liu, Yueping Open Life Sci Research Article Biocontrol is a cost-effective and environmentally friendly technique used in agricultural production. We isolated and screened a bacterial strain from the soils of a peach orchard with high yield. Using biochemical and physiological analysis as well as phylogenetic sequencing data, we identified a strain of Bacillus methylotrophicus, strain XJ-C. The results of our screening trials showed that XJ-C was able to suppress M. fructicola at an inhibition rate of 81.57%. Following the application of a 1×10(9) CFU/mL XJ-C strain suspension to the fruits, leaves, and shoots of peach trees infected with M. fructicola, the inhibition rate reached 64.31%, 97.34%, and 64.28%, respectively. Using OM and SEM, we observed that, under the inhibition of strain XJ-C, M. fructicola mycelium and spores were abnormally shaped. Under TEM, cell walls were transparent, organelles had disappeared, and the intracellular vacuole was deformed. Thus, XJ-C has the potential to be used in biocontrol. De Gruyter 2019-11-06 /pmc/articles/PMC7874823/ /pubmed/33817176 http://dx.doi.org/10.1515/biol-2019-0046 Text en © 2019 Xue Yuan, et al., published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 Public License.
spellingShingle Research Article
Yuan, Xue
Hou, Xu
Chang, Haotian
Yang, Rui
Wang, Fang
Liu, Yueping
Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title_full Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title_fullStr Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title_full_unstemmed Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title_short Bacillus Methylotrophicus Has Potential Applications Against Monilinia Fructicola
title_sort bacillus methylotrophicus has potential applications against monilinia fructicola
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874823/
https://www.ncbi.nlm.nih.gov/pubmed/33817176
http://dx.doi.org/10.1515/biol-2019-0046
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