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A set of shuttle plasmids for gene expression in Acinetobacter baumannii

Infections caused by the emerging opportunistic bacterial pathogen Acinetobacter baumannii are occurring at increasingly alarming rates, and such increase in incidence is further compounded by the development of wide spread multidrug-resistant strains. Yet, our understanding of its pathogenesis and...

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Autores principales: Jie, Jing, Chu, Xiao, Li, Dan, Luo, Zhaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875395/
https://www.ncbi.nlm.nih.gov/pubmed/33566854
http://dx.doi.org/10.1371/journal.pone.0246918
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author Jie, Jing
Chu, Xiao
Li, Dan
Luo, Zhaoqing
author_facet Jie, Jing
Chu, Xiao
Li, Dan
Luo, Zhaoqing
author_sort Jie, Jing
collection PubMed
description Infections caused by the emerging opportunistic bacterial pathogen Acinetobacter baumannii are occurring at increasingly alarming rates, and such increase in incidence is further compounded by the development of wide spread multidrug-resistant strains. Yet, our understanding of its pathogenesis and biology remains limited which can be attributed in part to the scarce of tools for molecular genetic analysis of this bacterium. Plasmids based on pWH1277 originally isolated from Acinetobacter calcoaceticus are the only vehicles currently available for ectopic gene expression in Acinetobacter species, which restricts experiments that require simultaneous analysis of multiple genes. Here, we found that plasmids of the IncQ group are able to replicate in A. baumannii and can stably co-reside with derivatives of pWH1277. Furthermore, we have constructed a series of four plasmids that allow inducible expression of Flag-tagged proteins in A. baumannii by arabinose or isopropyl β-d-1-thiogalactopyranoside. Together with constructs previously developed, these plasmids will accommodate the need in genetic analysis of this increasingly important pathogen.
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spelling pubmed-78753952021-02-19 A set of shuttle plasmids for gene expression in Acinetobacter baumannii Jie, Jing Chu, Xiao Li, Dan Luo, Zhaoqing PLoS One Research Article Infections caused by the emerging opportunistic bacterial pathogen Acinetobacter baumannii are occurring at increasingly alarming rates, and such increase in incidence is further compounded by the development of wide spread multidrug-resistant strains. Yet, our understanding of its pathogenesis and biology remains limited which can be attributed in part to the scarce of tools for molecular genetic analysis of this bacterium. Plasmids based on pWH1277 originally isolated from Acinetobacter calcoaceticus are the only vehicles currently available for ectopic gene expression in Acinetobacter species, which restricts experiments that require simultaneous analysis of multiple genes. Here, we found that plasmids of the IncQ group are able to replicate in A. baumannii and can stably co-reside with derivatives of pWH1277. Furthermore, we have constructed a series of four plasmids that allow inducible expression of Flag-tagged proteins in A. baumannii by arabinose or isopropyl β-d-1-thiogalactopyranoside. Together with constructs previously developed, these plasmids will accommodate the need in genetic analysis of this increasingly important pathogen. Public Library of Science 2021-02-10 /pmc/articles/PMC7875395/ /pubmed/33566854 http://dx.doi.org/10.1371/journal.pone.0246918 Text en © 2021 Jie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jie, Jing
Chu, Xiao
Li, Dan
Luo, Zhaoqing
A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title_full A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title_fullStr A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title_full_unstemmed A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title_short A set of shuttle plasmids for gene expression in Acinetobacter baumannii
title_sort set of shuttle plasmids for gene expression in acinetobacter baumannii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875395/
https://www.ncbi.nlm.nih.gov/pubmed/33566854
http://dx.doi.org/10.1371/journal.pone.0246918
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