Cargando…

Automatic deep learning-driven label-free image-guided patch clamp system

Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here, we demonstrate a tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection of cells in label-free images, calibration of the micropi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Koos, Krisztian, Oláh, Gáspár, Balassa, Tamas, Mihut, Norbert, Rózsa, Márton, Ozsvár, Attila, Tasnadi, Ervin, Barzó, Pál, Faragó, Nóra, Puskás, László, Molnár, Gábor, Molnár, József, Tamás, Gábor, Horvath, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875980/ https://www.ncbi.nlm.nih.gov/pubmed/33568670 http://dx.doi.org/10.1038/s41467-021-21291-4

Ejemplares similares

Human neuronal changes in brain edema and increased intracranial pressure
por: Faragó, Nóra, et al.
Publicado: (2016)

Predominantly linear summation of metabotropic postsynaptic potentials follows coactivation of neurogliaform interneurons
por: Ozsvár, Attila, et al.
Publicado: (2021)

Human pyramidal to interneuron synapses are mediated by multi-vesicular release and multiple docked vesicles
por: Molnár, Gábor, et al.
Publicado: (2016)

Temporal disparity of action potentials triggered in axon initial segments and distal axons in the neocortex
por: Rózsa, Márton, et al.
Publicado: (2023)

DIC image reconstruction using an energy minimization framework to visualize optical path length distribution
por: Koos, Krisztian, et al.
Publicado: (2016)

Complex Events Initiated by Individual Spikes in the Human Cerebral Cortex
por: Molnár, Gábor, et al.
Publicado: (2008)

Intelligent image-based in situ single-cell isolation
por: Brasko, Csilla, et al.
Publicado: (2018)

Software tools for 3D nuclei segmentation and quantitative analysis in multicellular aggregates
por: Piccinini, Filippo, et al.
Publicado: (2020)

Plasticity in Single Axon Glutamatergic Connection to GABAergic Interneurons Regulates Complex Events in the Human Neocortex
por: Szegedi, Viktor, et al.
Publicado: (2016)

High-Precision Fast-Spiking Basket Cell Discharges during Complex Events in the Human Neocortex
por: Szegedi, Viktor, et al.
Publicado: (2017)

Robust perisomatic GABAergic self-innervation inhibits basket cells in the human and mouse supragranular neocortex
por: Szegedi, Viktor, et al.
Publicado: (2020)

Molecular and Electrophysiological Characterization of GABAergic Interneurons Expressing the Transcription Factor COUP-TFII in the Adult Human Temporal Cortex
por: Varga, Csaba, et al.
Publicado: (2015)

nucleAIzer: A Parameter-free Deep Learning Framework for Nucleus Segmentation Using Image Style Transfer
por: Hollandi, Reka, et al.
Publicado: (2020)

Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type
por: Boldog, Eszter, et al.
Publicado: (2018)

Output of Neurogliaform Cells to Various Neuron Types in the Human and Rat Cerebral Cortex
por: Oláh, Szabolcs, et al.
Publicado: (2007)

Regulation of cortical microcircuits by unitary GABAergic volume transmission
por: Oláh, Szabolcs, et al.
Publicado: (2009)

A deep convolutional neural network approach for astrocyte detection
por: Suleymanova, Ilida, et al.
Publicado: (2018)

3D-Cell-Annotator: an open-source active surface tool for single-cell segmentation in 3D microscopy images
por: Tasnadi, Ervin A, et al.
Publicado: (2020)

Current translational potential and underlying molecular mechanisms of necroptosis
por: Molnár, Tamás, et al.
Publicado: (2019)

Automatic tracking of cells for video microscopy in patch clamp experiments
por: Peixoto, Helton M, et al.
Publicado: (2014)

Environmental properties of cells improve machine learning-based phenotype recognition accuracy
por: Toth, Timea, et al.
Publicado: (2018)

Types of necroinflammation, the effect of cell death modalities on sterile inflammation
por: Mázló, Anett, et al.
Publicado: (2022)

Strigolactones Interact With Nitric Oxide in Regulating Root System Architecture of Arabidopsis thaliana
por: Oláh, Dóra, et al.
Publicado: (2020)

Deuterium-depleted water stimulates GLUT4 translocation in the presence of insulin, which leads to decreased blood glucose concentration
por: Molnár, Miklós, et al.
Publicado: (2021)

Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
por: Vollár, Martin, et al.
Publicado: (2020)

An Advanced Automated Patch Clamp Protocol Design to Investigate Drug—Ion Channel Binding Dynamics
por: Lukacs, Peter, et al.
Publicado: (2021)

The acoustic bases of human voice identity processing in dogs
por: Gábor, Anna, et al.
Publicado: (2022)

Machine Learning-Based Pipette Positional Correction for Automatic Patch Clamp In Vitro
por: Gonzalez, Mercedes M., et al.
Publicado: (2021)

On the Face of It: No Differential Sensitivity to Internal Facial Features in the Dog Brain
por: Szabó, Dóra, et al.
Publicado: (2020)

Hsp70-associated chaperones have a critical role in buffering protein production costs
por: Farkas, Zoltán, et al.
Publicado: (2018)

An Extensive Study of Phenol Red Thread as a Novel Non-Invasive Tear Sampling Technique for Proteomics Studies: Comparison with Two Commonly Used Methods
por: Kecskeméti, Gábor, et al.
Publicado: (2022)

Semi-automatic FISH quantification on digital slides
por: Kiszler, Gábor, et al.
Publicado: (2013)

Gigaseal formation in patch clamping: with applications of nanotechnology
por: Malboubi, Majid, et al.
Publicado: (2014)

Cleaning patch-clamp pipettes for immediate reuse
por: Kolb, I., et al.
Publicado: (2016)

Patch clamp-assisted single neuron lipidomics
por: Merrill, Collin B., et al.
Publicado: (2017)

Progress in automating patch clamp cellular physiology
por: Annecchino, Luca A., et al.
Publicado: (2018)

Nanotechnology: new opportunities for the development of patch‐clamps
por: Gao, Jia, et al.
Publicado: (2021)

Stabilization of nanosized titanium dioxide by cyclodextrin polymers and its photocatalytic effect on the degradation of wastewater pollutants
por: Agócs, Tamás Zoltán, et al.
Publicado: (2016)

Structure preserving adversarial generation of labeled training samples for single-cell segmentation
por: Tasnadi, Ervin, et al.
Publicado: (2023)

The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus
por: Mátyás, Csaba, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_mjmoc3q95aqmue55fl22ukf24l