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PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a sm...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875988/ https://www.ncbi.nlm.nih.gov/pubmed/33568708 http://dx.doi.org/10.1038/s41598-021-83189-x |
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author | Dabaghi, Mohammadhossein Saraei, Neda Xu, Gang Chandiramohan, Abiram Yeung, Jonas Nguyen, Jenny P. Vukmirovic, Milica Selvaganapathy, Ponnambalam Ravi Hirota, Jeremy A. |
author_facet | Dabaghi, Mohammadhossein Saraei, Neda Xu, Gang Chandiramohan, Abiram Yeung, Jonas Nguyen, Jenny P. Vukmirovic, Milica Selvaganapathy, Ponnambalam Ravi Hirota, Jeremy A. |
author_sort | Dabaghi, Mohammadhossein |
collection | PubMed |
description | In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR—a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air–liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenge conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wire-based sensors have the capacity for miniaturization and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings. |
format | Online Article Text |
id | pubmed-7875988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78759882021-02-11 PHAIR: a biosensor for pH measurement in air–liquid interface cell culture Dabaghi, Mohammadhossein Saraei, Neda Xu, Gang Chandiramohan, Abiram Yeung, Jonas Nguyen, Jenny P. Vukmirovic, Milica Selvaganapathy, Ponnambalam Ravi Hirota, Jeremy A. Sci Rep Article In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR—a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air–liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenge conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wire-based sensors have the capacity for miniaturization and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings. Nature Publishing Group UK 2021-02-10 /pmc/articles/PMC7875988/ /pubmed/33568708 http://dx.doi.org/10.1038/s41598-021-83189-x Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dabaghi, Mohammadhossein Saraei, Neda Xu, Gang Chandiramohan, Abiram Yeung, Jonas Nguyen, Jenny P. Vukmirovic, Milica Selvaganapathy, Ponnambalam Ravi Hirota, Jeremy A. PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title | PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title_full | PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title_fullStr | PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title_full_unstemmed | PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title_short | PHAIR: a biosensor for pH measurement in air–liquid interface cell culture |
title_sort | phair: a biosensor for ph measurement in air–liquid interface cell culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7875988/ https://www.ncbi.nlm.nih.gov/pubmed/33568708 http://dx.doi.org/10.1038/s41598-021-83189-x |
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