Cargando…

Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs

We investigated the effect of diets containing organic zinc and a mixture of medicinal herbs on ruminal microbial fermentation and histopathology in lambs. Twenty-eight lambs were divided into four groups: unsupplemented animals (Control), animals supplemented with organic zinc (Zn, 70 mg Zn/kg diet...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrič, Daniel, Mravčáková, Dominika, Kucková, Katarína, Kišidayová, Svetlana, Cieslak, Adam, Szumacher-Strabel, Malgorzata, Huang, Haihao, Kolodziejski, Pawel, Lukomska, Anna, Slusarczyk, Sylwester, Čobanová, Klaudia, Váradyová, Zora
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876273/
https://www.ncbi.nlm.nih.gov/pubmed/33585621
http://dx.doi.org/10.3389/fvets.2021.630971
_version_ 1783649937297244160
author Petrič, Daniel
Mravčáková, Dominika
Kucková, Katarína
Kišidayová, Svetlana
Cieslak, Adam
Szumacher-Strabel, Malgorzata
Huang, Haihao
Kolodziejski, Pawel
Lukomska, Anna
Slusarczyk, Sylwester
Čobanová, Klaudia
Váradyová, Zora
author_facet Petrič, Daniel
Mravčáková, Dominika
Kucková, Katarína
Kišidayová, Svetlana
Cieslak, Adam
Szumacher-Strabel, Malgorzata
Huang, Haihao
Kolodziejski, Pawel
Lukomska, Anna
Slusarczyk, Sylwester
Čobanová, Klaudia
Váradyová, Zora
author_sort Petrič, Daniel
collection PubMed
description We investigated the effect of diets containing organic zinc and a mixture of medicinal herbs on ruminal microbial fermentation and histopathology in lambs. Twenty-eight lambs were divided into four groups: unsupplemented animals (Control), animals supplemented with organic zinc (Zn, 70 mg Zn/kg diet), animals supplemented with a mixture of dry medicinal herbs (Herbs, 100 g dry matter (DM)/d) and animals supplemented with both zinc and herbs (Zn+Herbs). Each lamb was fed a basal diet composed of meadow hay (700 g DM/d) and barley (300 g DM/d). The herbs Fumaria officinalis L. (FO), Malva sylvestris L. (MS), Artemisia absinthium L. (AA) and Matricaria chamomilla L. (MC) were mixed in equal proportions. The lambs were slaughtered after 70 d. The ruminal contents were used to determine the parameters of fermentation in vitro and in vivo and to quantify the microbes by molecular and microscopic methods. Samples of fresh ruminal tissue were used for histopathological evaluation. Quantitative analyses of the bioactive compounds in FO, MS, AA, and MC identified 3.961, 0.654, 6.482, and 12.084 g/kg DM phenolic acids and 12.211, 6.479, 0.349, and 2.442 g/kg DM flavonoids, respectively. The alkaloid content in FO was 6.015 g/kg DM. The diets affected the levels of total gas, methane and n-butyrate in vitro (P < 0.046, < 0.001, and < 0.001, respectively). Relative quantification by real-time PCR indicated a lower total ruminal bacterial population in the lambs in the Zn and Zn+Herbs groups than the Control group (P < 0.05). The relative abundances of Ruminococcus albus, R. flavefaciens, Streptococcus bovis, and Butyrivibrio proteoclasticus shifted in the Zn group. Morphological observation found a focally mixed infiltration of inflammatory cells in the lamina propria of the rumen in the Zn+Herbs group. The effect of the organic zinc and the herbal mixture on the parameters of ruminal fermentation in vitro was not confirmed in vivo, perhaps because the ruminal microbiota of the lambs adapted to the zinc-supplemented diets. Long-term supplementation of a diet combining zinc and medicinal herbs, however, may negatively affect the health of the ruminal epithelium of lambs.
format Online
Article
Text
id pubmed-7876273
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78762732021-02-12 Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs Petrič, Daniel Mravčáková, Dominika Kucková, Katarína Kišidayová, Svetlana Cieslak, Adam Szumacher-Strabel, Malgorzata Huang, Haihao Kolodziejski, Pawel Lukomska, Anna Slusarczyk, Sylwester Čobanová, Klaudia Váradyová, Zora Front Vet Sci Veterinary Science We investigated the effect of diets containing organic zinc and a mixture of medicinal herbs on ruminal microbial fermentation and histopathology in lambs. Twenty-eight lambs were divided into four groups: unsupplemented animals (Control), animals supplemented with organic zinc (Zn, 70 mg Zn/kg diet), animals supplemented with a mixture of dry medicinal herbs (Herbs, 100 g dry matter (DM)/d) and animals supplemented with both zinc and herbs (Zn+Herbs). Each lamb was fed a basal diet composed of meadow hay (700 g DM/d) and barley (300 g DM/d). The herbs Fumaria officinalis L. (FO), Malva sylvestris L. (MS), Artemisia absinthium L. (AA) and Matricaria chamomilla L. (MC) were mixed in equal proportions. The lambs were slaughtered after 70 d. The ruminal contents were used to determine the parameters of fermentation in vitro and in vivo and to quantify the microbes by molecular and microscopic methods. Samples of fresh ruminal tissue were used for histopathological evaluation. Quantitative analyses of the bioactive compounds in FO, MS, AA, and MC identified 3.961, 0.654, 6.482, and 12.084 g/kg DM phenolic acids and 12.211, 6.479, 0.349, and 2.442 g/kg DM flavonoids, respectively. The alkaloid content in FO was 6.015 g/kg DM. The diets affected the levels of total gas, methane and n-butyrate in vitro (P < 0.046, < 0.001, and < 0.001, respectively). Relative quantification by real-time PCR indicated a lower total ruminal bacterial population in the lambs in the Zn and Zn+Herbs groups than the Control group (P < 0.05). The relative abundances of Ruminococcus albus, R. flavefaciens, Streptococcus bovis, and Butyrivibrio proteoclasticus shifted in the Zn group. Morphological observation found a focally mixed infiltration of inflammatory cells in the lamina propria of the rumen in the Zn+Herbs group. The effect of the organic zinc and the herbal mixture on the parameters of ruminal fermentation in vitro was not confirmed in vivo, perhaps because the ruminal microbiota of the lambs adapted to the zinc-supplemented diets. Long-term supplementation of a diet combining zinc and medicinal herbs, however, may negatively affect the health of the ruminal epithelium of lambs. Frontiers Media S.A. 2021-01-28 /pmc/articles/PMC7876273/ /pubmed/33585621 http://dx.doi.org/10.3389/fvets.2021.630971 Text en Copyright © 2021 Petrič, Mravčáková, Kucková, Kišidayová, Cieslak, Szumacher-Strabel, Huang, Kolodziejski, Lukomska, Slusarczyk, Čobanová and Váradyová. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Petrič, Daniel
Mravčáková, Dominika
Kucková, Katarína
Kišidayová, Svetlana
Cieslak, Adam
Szumacher-Strabel, Malgorzata
Huang, Haihao
Kolodziejski, Pawel
Lukomska, Anna
Slusarczyk, Sylwester
Čobanová, Klaudia
Váradyová, Zora
Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title_full Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title_fullStr Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title_full_unstemmed Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title_short Impact of Zinc and/or Herbal Mixture on Ruminal Fermentation, Microbiota, and Histopathology in Lambs
title_sort impact of zinc and/or herbal mixture on ruminal fermentation, microbiota, and histopathology in lambs
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876273/
https://www.ncbi.nlm.nih.gov/pubmed/33585621
http://dx.doi.org/10.3389/fvets.2021.630971
work_keys_str_mv AT petricdaniel impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT mravcakovadominika impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT kuckovakatarina impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT kisidayovasvetlana impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT cieslakadam impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT szumacherstrabelmalgorzata impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT huanghaihao impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT kolodziejskipawel impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT lukomskaanna impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT slusarczyksylwester impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT cobanovaklaudia impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs
AT varadyovazora impactofzincandorherbalmixtureonruminalfermentationmicrobiotaandhistopathologyinlambs