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Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification

End-stage renal disease (ESRD) patients usually develop extensive and progressive vascular calcification, and lots of calcification inhibitors as well as procalcifying factors are involved in the process. However, the mechanisms of vascular calcification in ESRD patients are still ill-defined. In th...

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Autores principales: Lin, Xiao, Zhu, Ting, Xu, Feng, Zhong, Jia-Yu, Li, Fuxingzi, Shan, Su-Kang, Wu, Feng, Guo, Bei, Zheng, Ming-Hui, Wang, Yi, Xu, Qiu-Shuang, Liao, Xiao-Bo, Lu, Hong-Yu, Xie, Xu-Biao, Yuan, Ling-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876285/
https://www.ncbi.nlm.nih.gov/pubmed/33585452
http://dx.doi.org/10.3389/fcell.2020.618228
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author Lin, Xiao
Zhu, Ting
Xu, Feng
Zhong, Jia-Yu
Li, Fuxingzi
Shan, Su-Kang
Wu, Feng
Guo, Bei
Zheng, Ming-Hui
Wang, Yi
Xu, Qiu-Shuang
Liao, Xiao-Bo
Lu, Hong-Yu
Xie, Xu-Biao
Yuan, Ling-Qing
author_facet Lin, Xiao
Zhu, Ting
Xu, Feng
Zhong, Jia-Yu
Li, Fuxingzi
Shan, Su-Kang
Wu, Feng
Guo, Bei
Zheng, Ming-Hui
Wang, Yi
Xu, Qiu-Shuang
Liao, Xiao-Bo
Lu, Hong-Yu
Xie, Xu-Biao
Yuan, Ling-Qing
author_sort Lin, Xiao
collection PubMed
description End-stage renal disease (ESRD) patients usually develop extensive and progressive vascular calcification, and lots of calcification inhibitors as well as procalcifying factors are involved in the process. However, the mechanisms of vascular calcification in ESRD patients are still ill-defined. In the present study, we found that the plasma exosomes derived from ESRD patients (ESRD-Ex) promoted calcification of vascular smooth muscle cells (VSMCs) significantly, while plasma exosomes from renal transplant recipients (RTR-Ex) could partially attenuate VSMCs calcification. Moreover, the protein concentration of ESRD-Ex was significantly higher than plasma exosomes from the normal health control group (Nor-Ex) and RTR-Ex, and the content of both matrix gla protein (MGP) and Fetuin-A, the calcification inhibitors, were prominently lower in ESRD-Ex than those in Nor-Ex. The content of Annexin-A2, one of the calcification promoters, was significantly higher in ESRD-Ex and RTR-Ex than that in Nor-Ex. However, bone morphogenetic protein (BMP-2) and receptor activator for nuclear factor-κB ligand (Rankl) had no significant difference among the three groups. In addition, the content of Fetuin-A in RTR-Ex was higher than that in ESRD-Ex, although it was still lower than that in Nor-Ex. Furthermore, the levels of both Fetuin-A and MGP in plasma exosomes were negatively while the levels of Annexin-A2 in plasma exosomes was positively correlated to coronary artery calcification scores (CACS). These results indicated that ESRD-Ex significantly promoted VSMCs calcification, while renal transplantation could partially attenuate the procalcification effect of exosomes. Fetuin-A and MGP were decreased, but Annexin-A2 was increased in ESRD-Ex, and renal transplantation could increase the level of Fetuin-A rather than MGP.
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spelling pubmed-78762852021-02-12 Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification Lin, Xiao Zhu, Ting Xu, Feng Zhong, Jia-Yu Li, Fuxingzi Shan, Su-Kang Wu, Feng Guo, Bei Zheng, Ming-Hui Wang, Yi Xu, Qiu-Shuang Liao, Xiao-Bo Lu, Hong-Yu Xie, Xu-Biao Yuan, Ling-Qing Front Cell Dev Biol Cell and Developmental Biology End-stage renal disease (ESRD) patients usually develop extensive and progressive vascular calcification, and lots of calcification inhibitors as well as procalcifying factors are involved in the process. However, the mechanisms of vascular calcification in ESRD patients are still ill-defined. In the present study, we found that the plasma exosomes derived from ESRD patients (ESRD-Ex) promoted calcification of vascular smooth muscle cells (VSMCs) significantly, while plasma exosomes from renal transplant recipients (RTR-Ex) could partially attenuate VSMCs calcification. Moreover, the protein concentration of ESRD-Ex was significantly higher than plasma exosomes from the normal health control group (Nor-Ex) and RTR-Ex, and the content of both matrix gla protein (MGP) and Fetuin-A, the calcification inhibitors, were prominently lower in ESRD-Ex than those in Nor-Ex. The content of Annexin-A2, one of the calcification promoters, was significantly higher in ESRD-Ex and RTR-Ex than that in Nor-Ex. However, bone morphogenetic protein (BMP-2) and receptor activator for nuclear factor-κB ligand (Rankl) had no significant difference among the three groups. In addition, the content of Fetuin-A in RTR-Ex was higher than that in ESRD-Ex, although it was still lower than that in Nor-Ex. Furthermore, the levels of both Fetuin-A and MGP in plasma exosomes were negatively while the levels of Annexin-A2 in plasma exosomes was positively correlated to coronary artery calcification scores (CACS). These results indicated that ESRD-Ex significantly promoted VSMCs calcification, while renal transplantation could partially attenuate the procalcification effect of exosomes. Fetuin-A and MGP were decreased, but Annexin-A2 was increased in ESRD-Ex, and renal transplantation could increase the level of Fetuin-A rather than MGP. Frontiers Media S.A. 2021-01-28 /pmc/articles/PMC7876285/ /pubmed/33585452 http://dx.doi.org/10.3389/fcell.2020.618228 Text en Copyright © 2021 Lin, Zhu, Xu, Zhong, Li, Shan, Wu, Guo, Zheng, Wang, Xu, Liao, Lu, Xie and Yuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Lin, Xiao
Zhu, Ting
Xu, Feng
Zhong, Jia-Yu
Li, Fuxingzi
Shan, Su-Kang
Wu, Feng
Guo, Bei
Zheng, Ming-Hui
Wang, Yi
Xu, Qiu-Shuang
Liao, Xiao-Bo
Lu, Hong-Yu
Xie, Xu-Biao
Yuan, Ling-Qing
Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title_full Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title_fullStr Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title_full_unstemmed Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title_short Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification
title_sort plasma exosomes derived from patients with end-stage renal disease and renal transplant recipients have different effects on vascular calcification
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876285/
https://www.ncbi.nlm.nih.gov/pubmed/33585452
http://dx.doi.org/10.3389/fcell.2020.618228
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