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Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken
Bryophyllum pinnatum is a perennial herb traditionally used in ethnomedicine. In the present report, silver nanoparticles (AgNPs) were synthesized using B. pinnatum leaf extract. BP-AgNPs were confirmed following UV-Vis spectroscopy with SPR peak at 412 nm and further characterized by FTIR, XRD, SEM...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876318/ https://www.ncbi.nlm.nih.gov/pubmed/33585553 http://dx.doi.org/10.3389/fmolb.2020.593040 |
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author | Chandraker, Sandip Kumar Lal, Mishri Dhruve, Preeti Singh, Rana P. Shukla, Ravindra |
author_facet | Chandraker, Sandip Kumar Lal, Mishri Dhruve, Preeti Singh, Rana P. Shukla, Ravindra |
author_sort | Chandraker, Sandip Kumar |
collection | PubMed |
description | Bryophyllum pinnatum is a perennial herb traditionally used in ethnomedicine. In the present report, silver nanoparticles (AgNPs) were synthesized using B. pinnatum leaf extract. BP-AgNPs were confirmed following UV-Vis spectroscopy with SPR peak at 412 nm and further characterized by FTIR, XRD, SEM-EDX, and TEM. Microscopic images confirmed the spherical shape and ~15 nm average size of nanostructures. BP-AgNPs were evaluated for photocatalytic degradation of hazardous dyes (methylene blue and Rhodamine-B) and showed their complete reduction within 100 and 110 min., respectively. BP-AgNPs have emerged as a unique SPR-based novel sensor for the detection of H(2)O(2), which may deliver exciting prospects in clinical and industrial areas. DPPH and ABTS free radical scavenging activity were studied with respective IC(50) values of 89 and 259 μg/mL. A strong intercalating interaction of CT-DNA with BP-AgNPs was investigated. Observed chromosomal abnormalities confirm the antimitotic potential of BP-AgNPs in the meristematic root tip. The cytotoxicity of BP-AgNPs against B16F10 (melanoma cell line) and A431 (squamous cell carcinoma cell line), was assessed with respective IC(50) values of 59.5 and 96.61 μg/ml after 24 h of treatment. The presented green synthetic approach provides a novel and new door for environmental, industrial, and biomedical applications. |
format | Online Article Text |
id | pubmed-7876318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78763182021-02-12 Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken Chandraker, Sandip Kumar Lal, Mishri Dhruve, Preeti Singh, Rana P. Shukla, Ravindra Front Mol Biosci Molecular Biosciences Bryophyllum pinnatum is a perennial herb traditionally used in ethnomedicine. In the present report, silver nanoparticles (AgNPs) were synthesized using B. pinnatum leaf extract. BP-AgNPs were confirmed following UV-Vis spectroscopy with SPR peak at 412 nm and further characterized by FTIR, XRD, SEM-EDX, and TEM. Microscopic images confirmed the spherical shape and ~15 nm average size of nanostructures. BP-AgNPs were evaluated for photocatalytic degradation of hazardous dyes (methylene blue and Rhodamine-B) and showed their complete reduction within 100 and 110 min., respectively. BP-AgNPs have emerged as a unique SPR-based novel sensor for the detection of H(2)O(2), which may deliver exciting prospects in clinical and industrial areas. DPPH and ABTS free radical scavenging activity were studied with respective IC(50) values of 89 and 259 μg/mL. A strong intercalating interaction of CT-DNA with BP-AgNPs was investigated. Observed chromosomal abnormalities confirm the antimitotic potential of BP-AgNPs in the meristematic root tip. The cytotoxicity of BP-AgNPs against B16F10 (melanoma cell line) and A431 (squamous cell carcinoma cell line), was assessed with respective IC(50) values of 59.5 and 96.61 μg/ml after 24 h of treatment. The presented green synthetic approach provides a novel and new door for environmental, industrial, and biomedical applications. Frontiers Media S.A. 2021-01-28 /pmc/articles/PMC7876318/ /pubmed/33585553 http://dx.doi.org/10.3389/fmolb.2020.593040 Text en Copyright © 2021 Chandraker, Lal, Dhruve, Singh and Shukla. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Chandraker, Sandip Kumar Lal, Mishri Dhruve, Preeti Singh, Rana P. Shukla, Ravindra Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title | Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title_full | Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title_fullStr | Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title_full_unstemmed | Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title_short | Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H(2)O(2) Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken |
title_sort | cytotoxic, antimitotic, dna binding, photocatalytic, h(2)o(2) sensing, and antioxidant properties of biofabricated silver nanoparticles using leaf extract of bryophyllum pinnatum (lam.) oken |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876318/ https://www.ncbi.nlm.nih.gov/pubmed/33585553 http://dx.doi.org/10.3389/fmolb.2020.593040 |
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