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Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome

BACKGROUND: Clinical experience showed that the majority of Torsade de Pointes (TdP) ventricular tachyarrhythmia (VT) in patients with long QT syndrome (LQTS) are self-terminating (ST), but the few that are non-self-terminating (NST) are potentially fatal. A paramount issue in clinical arrhythmology...

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Autores principales: Himel, Herman D., Cupelli, Michael, Boutjdir, Mohamed, El-Sherif, Nabil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876465/
https://www.ncbi.nlm.nih.gov/pubmed/33584347
http://dx.doi.org/10.3389/fphys.2021.617847
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author Himel, Herman D.
Cupelli, Michael
Boutjdir, Mohamed
El-Sherif, Nabil
author_facet Himel, Herman D.
Cupelli, Michael
Boutjdir, Mohamed
El-Sherif, Nabil
author_sort Himel, Herman D.
collection PubMed
description BACKGROUND: Clinical experience showed that the majority of Torsade de Pointes (TdP) ventricular tachyarrhythmia (VT) in patients with long QT syndrome (LQTS) are self-terminating (ST), but the few that are non-self-terminating (NST) are potentially fatal. A paramount issue in clinical arrhythmology is to understand the electrophysiological mechanism of ST vs. NST TdP VT. METHODS: We investigated the electrophysiological mechanism of ST vs. NST TdP VT in the guinea pig Anthopleurin-A experimental model of LQTS, a close surrogate model of congenital LQT3. We utilized simultaneous optical recordings of membrane voltage (V(m)) and intracellular calcium (Ca(i)) and a robust analytical method based on spatiotemporal entropy difference (E(d)) to investigate the hypothesis that early V(m)/Ca(i) uncoupling during TdP VT can play a primary role in perpetuation of VT episodes. RESULTS: We analyzed a total of 35 episodes of TdP VT from 14 guinea pig surrogate models of LQTS, including 23 ST and 12 NST VTs. E(d) values for NST VT were significantly higher than E(d) values for ST VT. Analysis of wave front topology during the early phase of ST VT showed the Ca(i) wave front following closely V(m) wave front consistent with a lower degree of E(d). In contrast, NST VT was associated with uncoupling of V(m)/Ca(i) wave fronts during the first 2 or 3 cycles of VT associated with early wave break propagation pattern. CONCLUSIONS: Utilizing a robust analytical method we showed that, in comparison to ST TdP VT, NST VT was consistently predated by early uncoupling of V(m)/Ca(i) that destabilized wave front propagation and can explain a sustained complex reentrant excitation pattern.
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spelling pubmed-78764652021-02-12 Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome Himel, Herman D. Cupelli, Michael Boutjdir, Mohamed El-Sherif, Nabil Front Physiol Physiology BACKGROUND: Clinical experience showed that the majority of Torsade de Pointes (TdP) ventricular tachyarrhythmia (VT) in patients with long QT syndrome (LQTS) are self-terminating (ST), but the few that are non-self-terminating (NST) are potentially fatal. A paramount issue in clinical arrhythmology is to understand the electrophysiological mechanism of ST vs. NST TdP VT. METHODS: We investigated the electrophysiological mechanism of ST vs. NST TdP VT in the guinea pig Anthopleurin-A experimental model of LQTS, a close surrogate model of congenital LQT3. We utilized simultaneous optical recordings of membrane voltage (V(m)) and intracellular calcium (Ca(i)) and a robust analytical method based on spatiotemporal entropy difference (E(d)) to investigate the hypothesis that early V(m)/Ca(i) uncoupling during TdP VT can play a primary role in perpetuation of VT episodes. RESULTS: We analyzed a total of 35 episodes of TdP VT from 14 guinea pig surrogate models of LQTS, including 23 ST and 12 NST VTs. E(d) values for NST VT were significantly higher than E(d) values for ST VT. Analysis of wave front topology during the early phase of ST VT showed the Ca(i) wave front following closely V(m) wave front consistent with a lower degree of E(d). In contrast, NST VT was associated with uncoupling of V(m)/Ca(i) wave fronts during the first 2 or 3 cycles of VT associated with early wave break propagation pattern. CONCLUSIONS: Utilizing a robust analytical method we showed that, in comparison to ST TdP VT, NST VT was consistently predated by early uncoupling of V(m)/Ca(i) that destabilized wave front propagation and can explain a sustained complex reentrant excitation pattern. Frontiers Media S.A. 2021-01-28 /pmc/articles/PMC7876465/ /pubmed/33584347 http://dx.doi.org/10.3389/fphys.2021.617847 Text en Copyright © 2021 Himel, Cupelli, Boutjdir and El-Sherif. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Himel, Herman D.
Cupelli, Michael
Boutjdir, Mohamed
El-Sherif, Nabil
Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title_full Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title_fullStr Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title_full_unstemmed Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title_short Voltage/Calcium Uncoupling Underlies Sustained Torsade de Pointes Ventricular Tachyarrhythmia in an Experimental Model of Long QT Syndrome
title_sort voltage/calcium uncoupling underlies sustained torsade de pointes ventricular tachyarrhythmia in an experimental model of long qt syndrome
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876465/
https://www.ncbi.nlm.nih.gov/pubmed/33584347
http://dx.doi.org/10.3389/fphys.2021.617847
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