Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis

Fertilization of an egg by multiple sperm presents one of the earliest and most prevalent obstacles to successful reproduction. Eggs employ multiple mechanisms to prevent sperm entry into the nascent zygote. The fast block to polyspermy uses a depolarization to inhibit sperm entry. For some external...

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Autores principales: Tembo, Maiwase, Sauer, Monica L, Wisner, Bennett W, Beleny, David O, Napolitano, Marc A, Carlson, Anne E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876547/
https://www.ncbi.nlm.nih.gov/pubmed/33598639
http://dx.doi.org/10.17912/micropub.biology.000365
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author Tembo, Maiwase
Sauer, Monica L
Wisner, Bennett W
Beleny, David O
Napolitano, Marc A
Carlson, Anne E
author_facet Tembo, Maiwase
Sauer, Monica L
Wisner, Bennett W
Beleny, David O
Napolitano, Marc A
Carlson, Anne E
author_sort Tembo, Maiwase
collection PubMed
description Fertilization of an egg by multiple sperm presents one of the earliest and most prevalent obstacles to successful reproduction. Eggs employ multiple mechanisms to prevent sperm entry into the nascent zygote. The fast block to polyspermy uses a depolarization to inhibit sperm entry. For some external fertilizers, fertilization and the fast block require actin polymerization. Here we explored whether the fast block to polyspermy in the external fertilizer, Xenopus laevis, requires actin polymerization. Inseminating in the presence of inhibitor cytochalasin B, here we demonstrate that actin polymerization is not required for the fast block to polyspermy in X. laevis.
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spelling pubmed-78765472021-02-16 Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis Tembo, Maiwase Sauer, Monica L Wisner, Bennett W Beleny, David O Napolitano, Marc A Carlson, Anne E MicroPubl Biol Materials and Reagents Fertilization of an egg by multiple sperm presents one of the earliest and most prevalent obstacles to successful reproduction. Eggs employ multiple mechanisms to prevent sperm entry into the nascent zygote. The fast block to polyspermy uses a depolarization to inhibit sperm entry. For some external fertilizers, fertilization and the fast block require actin polymerization. Here we explored whether the fast block to polyspermy in the external fertilizer, Xenopus laevis, requires actin polymerization. Inseminating in the presence of inhibitor cytochalasin B, here we demonstrate that actin polymerization is not required for the fast block to polyspermy in X. laevis. Caltech Library 2021-02-09 /pmc/articles/PMC7876547/ /pubmed/33598639 http://dx.doi.org/10.17912/micropub.biology.000365 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Tembo, Maiwase
Sauer, Monica L
Wisner, Bennett W
Beleny, David O
Napolitano, Marc A
Carlson, Anne E
Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title_full Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title_fullStr Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title_full_unstemmed Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title_short Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
title_sort actin polymerization is not required for the fast block to polyspermy in the african clawed frog, xenopus laevis
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876547/
https://www.ncbi.nlm.nih.gov/pubmed/33598639
http://dx.doi.org/10.17912/micropub.biology.000365
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