Protocol to prepare functional cellular nanovesicles with PD1 and TRAIL to boost antitumor response

Immunotherapy has achieved notable success in tumor treatment, but it is restricted to a small number of patients due to multiple immunosuppressive pathways in the tumor microenvironment. Here, we present a step-by-step protocol to prepare functional cellular nanovesicles from HEK293-FT cells displa...

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Detalles Bibliográficos
Autores principales: Yu, Peiwen, Zheng, Dongye, Zhang, Cuilin, Wu, Ming, Liu, Xiaolong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876612/
https://www.ncbi.nlm.nih.gov/pubmed/33604583
http://dx.doi.org/10.1016/j.xpro.2021.100324
Descripción
Sumario:Immunotherapy has achieved notable success in tumor treatment, but it is restricted to a small number of patients due to multiple immunosuppressive pathways in the tumor microenvironment. Here, we present a step-by-step protocol to prepare functional cellular nanovesicles from HEK293-FT cells displaying PD1 and TRAIL. TRAIL specifically induces immunogenic cancer cell death to initiate an immune response, and ectogenic PD1 blocks the PD1/PDL1 checkpoint signal to reactivate anergic tumor-specific CD8+ T cells. For complete details on the use and execution of this protocol, please refer to Wu et al. (2020).

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