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Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

OBJECTIVE: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. METHODS: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years...

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Autores principales: Susilowati, Suherni, Sardjito, Trilas, Mustofa, Imam, Widodo, Oky Setio, Kurnijasanti, Rochmah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876723/
https://www.ncbi.nlm.nih.gov/pubmed/32299169
http://dx.doi.org/10.5713/ajas.20.0025
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author Susilowati, Suherni
Sardjito, Trilas
Mustofa, Imam
Widodo, Oky Setio
Kurnijasanti, Rochmah
author_facet Susilowati, Suherni
Sardjito, Trilas
Mustofa, Imam
Widodo, Oky Setio
Kurnijasanti, Rochmah
author_sort Susilowati, Suherni
collection PubMed
description OBJECTIVE: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. METHODS: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination. RESULTS: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12). CONCLUSION: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.
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spelling pubmed-78767232021-02-22 Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients Susilowati, Suherni Sardjito, Trilas Mustofa, Imam Widodo, Oky Setio Kurnijasanti, Rochmah Anim Biosci Article OBJECTIVE: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows. METHODS: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination. RESULTS: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12). CONCLUSION: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows. Asian-Australasian Association of Animal Production Societies 2021-02 2020-04-12 /pmc/articles/PMC7876723/ /pubmed/32299169 http://dx.doi.org/10.5713/ajas.20.0025 Text en Copyright © 2021 by Animal Bioscience This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Susilowati, Suherni
Sardjito, Trilas
Mustofa, Imam
Widodo, Oky Setio
Kurnijasanti, Rochmah
Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title_full Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title_fullStr Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title_full_unstemmed Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title_short Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients
title_sort effect of green tea extract in extender of simmental bull semen on pregnancy rate of recipients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876723/
https://www.ncbi.nlm.nih.gov/pubmed/32299169
http://dx.doi.org/10.5713/ajas.20.0025
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