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miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway

Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR-146b...

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Detalles Bibliográficos
Autores principales: Ouyang, Bin, Pan, Ningfeng, Zhang, Haifeng, Xing, Chuanming, Ji, Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877004/
https://www.ncbi.nlm.nih.gov/pubmed/33649824
http://dx.doi.org/10.3892/or.2021.7966
Descripción
Sumario:Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR-146b-5p and an increase in the expression of its target gene Toll-like receptor 4 (TLR4) were first observed in GBC tissues. Further study demonstrated that an increase in TLR4 expression caused by a decrease in miR-146b-5p expression led to activation of nuclear factor (NF)-κB signaling. GBC cells were cultured in vitro, and it was observed that overexpression of miR-146b-5p effectively inhibited their viability, proliferation, migration and invasion, and increased their apoptosis. Using a BALB/c nude mouse xenograft model, it was demonstrated that overexpression of miR-146b-5p was sufficient to reduce tumor volume and alleviate pathological characteristics. Overall, the results of the present study indicated that the decrease in the expression of miR-146b-5p increased TLR4 expression and indirectly activated the NF-κB signaling pathway, thereby regulating the development of GBC.