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miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway
Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR-146b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877004/ https://www.ncbi.nlm.nih.gov/pubmed/33649824 http://dx.doi.org/10.3892/or.2021.7966 |
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author | Ouyang, Bin Pan, Ningfeng Zhang, Haifeng Xing, Chuanming Ji, Wu |
author_facet | Ouyang, Bin Pan, Ningfeng Zhang, Haifeng Xing, Chuanming Ji, Wu |
author_sort | Ouyang, Bin |
collection | PubMed |
description | Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR-146b-5p and an increase in the expression of its target gene Toll-like receptor 4 (TLR4) were first observed in GBC tissues. Further study demonstrated that an increase in TLR4 expression caused by a decrease in miR-146b-5p expression led to activation of nuclear factor (NF)-κB signaling. GBC cells were cultured in vitro, and it was observed that overexpression of miR-146b-5p effectively inhibited their viability, proliferation, migration and invasion, and increased their apoptosis. Using a BALB/c nude mouse xenograft model, it was demonstrated that overexpression of miR-146b-5p was sufficient to reduce tumor volume and alleviate pathological characteristics. Overall, the results of the present study indicated that the decrease in the expression of miR-146b-5p increased TLR4 expression and indirectly activated the NF-κB signaling pathway, thereby regulating the development of GBC. |
format | Online Article Text |
id | pubmed-7877004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-78770042021-02-24 miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway Ouyang, Bin Pan, Ningfeng Zhang, Haifeng Xing, Chuanming Ji, Wu Oncol Rep Articles Gallbladder cancer (GBC) is a carcinoma of the biliary tract, which is common in developing countries and is associated with a high fatality rate. The aim of the present study was to investigate the mechanisms underlying the occurrence and development of GBC. A decrease in the expression of miR-146b-5p and an increase in the expression of its target gene Toll-like receptor 4 (TLR4) were first observed in GBC tissues. Further study demonstrated that an increase in TLR4 expression caused by a decrease in miR-146b-5p expression led to activation of nuclear factor (NF)-κB signaling. GBC cells were cultured in vitro, and it was observed that overexpression of miR-146b-5p effectively inhibited their viability, proliferation, migration and invasion, and increased their apoptosis. Using a BALB/c nude mouse xenograft model, it was demonstrated that overexpression of miR-146b-5p was sufficient to reduce tumor volume and alleviate pathological characteristics. Overall, the results of the present study indicated that the decrease in the expression of miR-146b-5p increased TLR4 expression and indirectly activated the NF-κB signaling pathway, thereby regulating the development of GBC. D.A. Spandidos 2021-04 2021-02-03 /pmc/articles/PMC7877004/ /pubmed/33649824 http://dx.doi.org/10.3892/or.2021.7966 Text en Copyright: © Ouyang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ouyang, Bin Pan, Ningfeng Zhang, Haifeng Xing, Chuanming Ji, Wu miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title | miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title_full | miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title_fullStr | miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title_full_unstemmed | miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title_short | miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-κB pathway |
title_sort | mir-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting toll-like receptor 4 via the nuclear factor-κb pathway |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877004/ https://www.ncbi.nlm.nih.gov/pubmed/33649824 http://dx.doi.org/10.3892/or.2021.7966 |
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