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Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices
Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens(1). Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. H...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877670/ https://www.ncbi.nlm.nih.gov/pubmed/33584848 http://dx.doi.org/10.1038/s41566-020-0593-1 |
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author | Chazot, Cécile A. C. Nagelberg, Sara Rowlands, Christopher J. Scherer, Maik R. J. Coropceanu, Igor Broderick, Kurt Kim, Yunjo Bawendi, Moungi G. So, Peter Kolle, Mathias |
author_facet | Chazot, Cécile A. C. Nagelberg, Sara Rowlands, Christopher J. Scherer, Maik R. J. Coropceanu, Igor Broderick, Kurt Kim, Yunjo Bawendi, Moungi G. So, Peter Kolle, Mathias |
author_sort | Chazot, Cécile A. C. |
collection | PubMed |
description | Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens(1). Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae, and other marine organisms often requires specialized, bulky microscope systems, and expensive additional components, such as dark-field-compatible objectives or condensers(2,3). Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle illumination cone required for dark field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices, compatible with simple and compact light microscopes. |
format | Online Article Text |
id | pubmed-7877670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-78776702021-02-11 Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices Chazot, Cécile A. C. Nagelberg, Sara Rowlands, Christopher J. Scherer, Maik R. J. Coropceanu, Igor Broderick, Kurt Kim, Yunjo Bawendi, Moungi G. So, Peter Kolle, Mathias Nat Photonics Article Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens(1). Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae, and other marine organisms often requires specialized, bulky microscope systems, and expensive additional components, such as dark-field-compatible objectives or condensers(2,3). Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle illumination cone required for dark field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices, compatible with simple and compact light microscopes. 2020-02-24 2020-05 /pmc/articles/PMC7877670/ /pubmed/33584848 http://dx.doi.org/10.1038/s41566-020-0593-1 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Chazot, Cécile A. C. Nagelberg, Sara Rowlands, Christopher J. Scherer, Maik R. J. Coropceanu, Igor Broderick, Kurt Kim, Yunjo Bawendi, Moungi G. So, Peter Kolle, Mathias Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title | Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title_full | Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title_fullStr | Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title_full_unstemmed | Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title_short | Luminescent Surfaces with Tailored Angular Emission for Compact Dark-Field Imaging Devices |
title_sort | luminescent surfaces with tailored angular emission for compact dark-field imaging devices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877670/ https://www.ncbi.nlm.nih.gov/pubmed/33584848 http://dx.doi.org/10.1038/s41566-020-0593-1 |
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