Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment
Biofilm formation protects bacteria from antibiotics. Very little is known about the response of biofilm-dwelling bacteria to antibiotics at the single cell level. Here, we developed a cell-tracking approach to investigate how antibiotics affect structure and dynamics of colonies formed by the human...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877761/ https://www.ncbi.nlm.nih.gov/pubmed/33524048 http://dx.doi.org/10.1371/journal.ppat.1009251 |
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author | Cronenberg, Tom Hennes, Marc Wielert, Isabelle Maier, Berenike |
author_facet | Cronenberg, Tom Hennes, Marc Wielert, Isabelle Maier, Berenike |
author_sort | Cronenberg, Tom |
collection | PubMed |
description | Biofilm formation protects bacteria from antibiotics. Very little is known about the response of biofilm-dwelling bacteria to antibiotics at the single cell level. Here, we developed a cell-tracking approach to investigate how antibiotics affect structure and dynamics of colonies formed by the human pathogen Neisseria gonorrhoeae. Antibiotics targeting different cellular functions enlarge the cell volumes and modulate within-colony motility. Focusing on azithromycin and ceftriaxone, we identify changes in type 4 pilus (T4P) mediated cell-to-cell attraction as the molecular mechanism for different effects on motility. By using strongly attractive mutant strains, we reveal that the survivability under ceftriaxone treatment depends on motility. Combining our results, we find that sequential treatment with azithromycin and ceftriaxone is synergistic. Taken together, we demonstrate that antibiotics modulate T4P-mediated attractions and hence cell motility and colony fluidity. |
format | Online Article Text |
id | pubmed-7877761 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78777612021-02-19 Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment Cronenberg, Tom Hennes, Marc Wielert, Isabelle Maier, Berenike PLoS Pathog Research Article Biofilm formation protects bacteria from antibiotics. Very little is known about the response of biofilm-dwelling bacteria to antibiotics at the single cell level. Here, we developed a cell-tracking approach to investigate how antibiotics affect structure and dynamics of colonies formed by the human pathogen Neisseria gonorrhoeae. Antibiotics targeting different cellular functions enlarge the cell volumes and modulate within-colony motility. Focusing on azithromycin and ceftriaxone, we identify changes in type 4 pilus (T4P) mediated cell-to-cell attraction as the molecular mechanism for different effects on motility. By using strongly attractive mutant strains, we reveal that the survivability under ceftriaxone treatment depends on motility. Combining our results, we find that sequential treatment with azithromycin and ceftriaxone is synergistic. Taken together, we demonstrate that antibiotics modulate T4P-mediated attractions and hence cell motility and colony fluidity. Public Library of Science 2021-02-01 /pmc/articles/PMC7877761/ /pubmed/33524048 http://dx.doi.org/10.1371/journal.ppat.1009251 Text en © 2021 Cronenberg et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Cronenberg, Tom Hennes, Marc Wielert, Isabelle Maier, Berenike Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title | Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title_full | Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title_fullStr | Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title_full_unstemmed | Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title_short | Antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
title_sort | antibiotics modulate attractive interactions in bacterial colonies affecting survivability under combined treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877761/ https://www.ncbi.nlm.nih.gov/pubmed/33524048 http://dx.doi.org/10.1371/journal.ppat.1009251 |
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