Cargando…
Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Neuroscience Society
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878034/ https://www.ncbi.nlm.nih.gov/pubmed/33613890 http://dx.doi.org/10.32598/bcn.9.10.190 |
_version_ | 1783650274987999232 |
---|---|
author | Fahanik-Babaei, Javad Baluchnejadmojarad, Tourandokht Roghani, Mehrdad |
author_facet | Fahanik-Babaei, Javad Baluchnejadmojarad, Tourandokht Roghani, Mehrdad |
author_sort | Fahanik-Babaei, Javad |
collection | PubMed |
description | INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat model of AD. METHODS: The experimental groups in this research included the control with no treatment, sham-operated receiving the vehicle (normal saline), and Aβ-lesioned groups. For modeling AD, aggregated Aβ1-40 (10 μg/2 μl on each side) was injected into the hippocampal CA1. Three weeks later, Population Spike (PS) amplitude and slope ratios were determined at different Inter-pulse Intervals (IPI) of 10, 20, 30, and 50 ms as a valid indicator of the short-term presynaptic facilitation and/or depression. In addition, PS amplitude and slope were taken as an index of long-term synaptic plasticity after application of High-frequency Stimulation (HFS) to induce LTP in the medial perforant-dentate gyrus pathway. RESULTS: No significant differences were noted amongst the experimental groups regarding fEPSP slope and paired-pulse indices as indicators of short-term plasticity. In contrast, fEPSP slope and PS amplitude significantly decreased following the application of HFS in Aβ-injected group. In addition, there was no significant difference between the control and sham-operated groups regarding the mentioned parameters. CONCLUSION: Findings of this study clearly demonstrated that microinjection of Aβ1-40 into the CA1 could impair LTP in dentate gyrus but could not modify short-term plasticity. |
format | Online Article Text |
id | pubmed-7878034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Iranian Neuroscience Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-78780342021-02-18 Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease Fahanik-Babaei, Javad Baluchnejadmojarad, Tourandokht Roghani, Mehrdad Basic Clin Neurosci Research Paper INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat model of AD. METHODS: The experimental groups in this research included the control with no treatment, sham-operated receiving the vehicle (normal saline), and Aβ-lesioned groups. For modeling AD, aggregated Aβ1-40 (10 μg/2 μl on each side) was injected into the hippocampal CA1. Three weeks later, Population Spike (PS) amplitude and slope ratios were determined at different Inter-pulse Intervals (IPI) of 10, 20, 30, and 50 ms as a valid indicator of the short-term presynaptic facilitation and/or depression. In addition, PS amplitude and slope were taken as an index of long-term synaptic plasticity after application of High-frequency Stimulation (HFS) to induce LTP in the medial perforant-dentate gyrus pathway. RESULTS: No significant differences were noted amongst the experimental groups regarding fEPSP slope and paired-pulse indices as indicators of short-term plasticity. In contrast, fEPSP slope and PS amplitude significantly decreased following the application of HFS in Aβ-injected group. In addition, there was no significant difference between the control and sham-operated groups regarding the mentioned parameters. CONCLUSION: Findings of this study clearly demonstrated that microinjection of Aβ1-40 into the CA1 could impair LTP in dentate gyrus but could not modify short-term plasticity. Iranian Neuroscience Society 2020 2020-07-01 /pmc/articles/PMC7878034/ /pubmed/33613890 http://dx.doi.org/10.32598/bcn.9.10.190 Text en Copyright© 2020 Iranian Neuroscience Society This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
spellingShingle | Research Paper Fahanik-Babaei, Javad Baluchnejadmojarad, Tourandokht Roghani, Mehrdad Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title | Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title_full | Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title_fullStr | Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title_full_unstemmed | Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title_short | Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease |
title_sort | differential effect of amyloid beta1–40 on short-term and long-term plasticity in dentate gyrus of a rat model of alzheimer disease |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878034/ https://www.ncbi.nlm.nih.gov/pubmed/33613890 http://dx.doi.org/10.32598/bcn.9.10.190 |
work_keys_str_mv | AT fahanikbabaeijavad differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease AT baluchnejadmojaradtourandokht differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease AT roghanimehrdad differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease |