Cargando…

Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease

INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat...

Descripción completa

Detalles Bibliográficos
Autores principales: Fahanik-Babaei, Javad, Baluchnejadmojarad, Tourandokht, Roghani, Mehrdad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878034/
https://www.ncbi.nlm.nih.gov/pubmed/33613890
http://dx.doi.org/10.32598/bcn.9.10.190
_version_ 1783650274987999232
author Fahanik-Babaei, Javad
Baluchnejadmojarad, Tourandokht
Roghani, Mehrdad
author_facet Fahanik-Babaei, Javad
Baluchnejadmojarad, Tourandokht
Roghani, Mehrdad
author_sort Fahanik-Babaei, Javad
collection PubMed
description INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat model of AD. METHODS: The experimental groups in this research included the control with no treatment, sham-operated receiving the vehicle (normal saline), and Aβ-lesioned groups. For modeling AD, aggregated Aβ1-40 (10 μg/2 μl on each side) was injected into the hippocampal CA1. Three weeks later, Population Spike (PS) amplitude and slope ratios were determined at different Inter-pulse Intervals (IPI) of 10, 20, 30, and 50 ms as a valid indicator of the short-term presynaptic facilitation and/or depression. In addition, PS amplitude and slope were taken as an index of long-term synaptic plasticity after application of High-frequency Stimulation (HFS) to induce LTP in the medial perforant-dentate gyrus pathway. RESULTS: No significant differences were noted amongst the experimental groups regarding fEPSP slope and paired-pulse indices as indicators of short-term plasticity. In contrast, fEPSP slope and PS amplitude significantly decreased following the application of HFS in Aβ-injected group. In addition, there was no significant difference between the control and sham-operated groups regarding the mentioned parameters. CONCLUSION: Findings of this study clearly demonstrated that microinjection of Aβ1-40 into the CA1 could impair LTP in dentate gyrus but could not modify short-term plasticity.
format Online
Article
Text
id pubmed-7878034
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Iranian Neuroscience Society
record_format MEDLINE/PubMed
spelling pubmed-78780342021-02-18 Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease Fahanik-Babaei, Javad Baluchnejadmojarad, Tourandokht Roghani, Mehrdad Basic Clin Neurosci Research Paper INTRODUCTION: Synaptic plasticity is inappropriately affected by neurodegenerative diseases, including Alzheimer Disease (AD). In this study, we examined the effect of intrahippocampal amyloid-beta (Aβ1-40) on dentate gyrus Long-term Potentiation (LTP) and presynaptic short-term plasticity in a rat model of AD. METHODS: The experimental groups in this research included the control with no treatment, sham-operated receiving the vehicle (normal saline), and Aβ-lesioned groups. For modeling AD, aggregated Aβ1-40 (10 μg/2 μl on each side) was injected into the hippocampal CA1. Three weeks later, Population Spike (PS) amplitude and slope ratios were determined at different Inter-pulse Intervals (IPI) of 10, 20, 30, and 50 ms as a valid indicator of the short-term presynaptic facilitation and/or depression. In addition, PS amplitude and slope were taken as an index of long-term synaptic plasticity after application of High-frequency Stimulation (HFS) to induce LTP in the medial perforant-dentate gyrus pathway. RESULTS: No significant differences were noted amongst the experimental groups regarding fEPSP slope and paired-pulse indices as indicators of short-term plasticity. In contrast, fEPSP slope and PS amplitude significantly decreased following the application of HFS in Aβ-injected group. In addition, there was no significant difference between the control and sham-operated groups regarding the mentioned parameters. CONCLUSION: Findings of this study clearly demonstrated that microinjection of Aβ1-40 into the CA1 could impair LTP in dentate gyrus but could not modify short-term plasticity. Iranian Neuroscience Society 2020 2020-07-01 /pmc/articles/PMC7878034/ /pubmed/33613890 http://dx.doi.org/10.32598/bcn.9.10.190 Text en Copyright© 2020 Iranian Neuroscience Society This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Paper
Fahanik-Babaei, Javad
Baluchnejadmojarad, Tourandokht
Roghani, Mehrdad
Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title_full Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title_fullStr Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title_full_unstemmed Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title_short Differential Effect of Amyloid Beta1–40 on Short-term and Long-term Plasticity in Dentate Gyrus of a Rat Model of Alzheimer Disease
title_sort differential effect of amyloid beta1–40 on short-term and long-term plasticity in dentate gyrus of a rat model of alzheimer disease
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878034/
https://www.ncbi.nlm.nih.gov/pubmed/33613890
http://dx.doi.org/10.32598/bcn.9.10.190
work_keys_str_mv AT fahanikbabaeijavad differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease
AT baluchnejadmojaradtourandokht differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease
AT roghanimehrdad differentialeffectofamyloidbeta140onshorttermandlongtermplasticityindentategyrusofaratmodelofalzheimerdisease