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Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism

Exposure to total body irradiation (TBI) causes dose- and tissue-specific lethality. However, there are few effective and nontoxic radiation countermeasures for the radiation injury. In the current study, mice were pretreated with a traditional antimicrobial agent, FZD, before TBI; the protective ef...

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Autores principales: Ma, Shumei, Jin, Zhao, Liu, Yi, Liu, Lin, Feng, Hao, Li, Ping, Tian, Zhujun, Ren, Minghua, Liu, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878070/
https://www.ncbi.nlm.nih.gov/pubmed/33613823
http://dx.doi.org/10.1155/2021/6610726
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author Ma, Shumei
Jin, Zhao
Liu, Yi
Liu, Lin
Feng, Hao
Li, Ping
Tian, Zhujun
Ren, Minghua
Liu, Xiaodong
author_facet Ma, Shumei
Jin, Zhao
Liu, Yi
Liu, Lin
Feng, Hao
Li, Ping
Tian, Zhujun
Ren, Minghua
Liu, Xiaodong
author_sort Ma, Shumei
collection PubMed
description Exposure to total body irradiation (TBI) causes dose- and tissue-specific lethality. However, there are few effective and nontoxic radiation countermeasures for the radiation injury. In the current study, mice were pretreated with a traditional antimicrobial agent, FZD, before TBI; the protective effects of FZD on radiation injury were evaluated by using parameters such as the spleen index and thymus index, immunohistochemical staining of intestinal tissue, and frequency of micronuclei in polychromatophilic erythrocytes of bone marrow. The intestinal epithelial cell line IEC-6 was used to investigate the underlying mechanisms. Our results indicated that FZD administration significantly improved the survival of lethal dose-irradiated mice, decreased the number of micronuclei, upregulated the number of leukocytes and immune organ indices, and restored intestinal integrity in mice after TBI. TUNEL and western blot showed that FZD protected intestinal tissue by downregulating radiation-induced apoptosis and autophagy. Meanwhile, FZD protected IEC-6 cells from radiation-induced cell death by inhibiting apoptosis and autophagy. To sum up, FZD protected against radiation-induced cell death both in vitro and in vivo through antiapoptosis and antiautophagy mechanisms.
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spelling pubmed-78780702021-02-19 Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism Ma, Shumei Jin, Zhao Liu, Yi Liu, Lin Feng, Hao Li, Ping Tian, Zhujun Ren, Minghua Liu, Xiaodong Oxid Med Cell Longev Research Article Exposure to total body irradiation (TBI) causes dose- and tissue-specific lethality. However, there are few effective and nontoxic radiation countermeasures for the radiation injury. In the current study, mice were pretreated with a traditional antimicrobial agent, FZD, before TBI; the protective effects of FZD on radiation injury were evaluated by using parameters such as the spleen index and thymus index, immunohistochemical staining of intestinal tissue, and frequency of micronuclei in polychromatophilic erythrocytes of bone marrow. The intestinal epithelial cell line IEC-6 was used to investigate the underlying mechanisms. Our results indicated that FZD administration significantly improved the survival of lethal dose-irradiated mice, decreased the number of micronuclei, upregulated the number of leukocytes and immune organ indices, and restored intestinal integrity in mice after TBI. TUNEL and western blot showed that FZD protected intestinal tissue by downregulating radiation-induced apoptosis and autophagy. Meanwhile, FZD protected IEC-6 cells from radiation-induced cell death by inhibiting apoptosis and autophagy. To sum up, FZD protected against radiation-induced cell death both in vitro and in vivo through antiapoptosis and antiautophagy mechanisms. Hindawi 2021-02-04 /pmc/articles/PMC7878070/ /pubmed/33613823 http://dx.doi.org/10.1155/2021/6610726 Text en Copyright © 2021 Shumei Ma et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ma, Shumei
Jin, Zhao
Liu, Yi
Liu, Lin
Feng, Hao
Li, Ping
Tian, Zhujun
Ren, Minghua
Liu, Xiaodong
Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title_full Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title_fullStr Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title_full_unstemmed Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title_short Furazolidone Increases Survival of Mice Exposed to Lethal Total Body Irradiation through the Antiapoptosis and Antiautophagy Mechanism
title_sort furazolidone increases survival of mice exposed to lethal total body irradiation through the antiapoptosis and antiautophagy mechanism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878070/
https://www.ncbi.nlm.nih.gov/pubmed/33613823
http://dx.doi.org/10.1155/2021/6610726
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