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Cold sensitivity of the SARS-CoV-2 spike ectodomain
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations, receptor-accessible “up” or receptor-inaccessible “down” states. Here we report that the commonly used stabilized S ectodomain construct “2P” is sensitive t...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878407/ https://www.ncbi.nlm.nih.gov/pubmed/33402708 http://dx.doi.org/10.1038/s41594-020-00547-5 |
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| author | Edwards, Robert J Mansouri, Katayoun Stalls, Victoria Manne, Kartik Watts, Brian Parks, Rob Janowska, Katarzyna Gobeil, Sophie M. C. Kopp, Megan Li, Dapeng Lu, Xiaozhi Mu, Zekun Deyton, Margaret Oguin, Thomas H Sprenz, Jordan Williams, Wilton Saunders, Kevin O. Montefiori, David Sempowski, Gregory D. Henderson, Rory Alam, S. Munir Haynes, Barton F. Acharya, Priyamvada |
| author_facet | Edwards, Robert J Mansouri, Katayoun Stalls, Victoria Manne, Kartik Watts, Brian Parks, Rob Janowska, Katarzyna Gobeil, Sophie M. C. Kopp, Megan Li, Dapeng Lu, Xiaozhi Mu, Zekun Deyton, Margaret Oguin, Thomas H Sprenz, Jordan Williams, Wilton Saunders, Kevin O. Montefiori, David Sempowski, Gregory D. Henderson, Rory Alam, S. Munir Haynes, Barton F. Acharya, Priyamvada |
| author_sort | Edwards, Robert J |
| collection | PubMed |
| description | The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations, receptor-accessible “up” or receptor-inaccessible “down” states. Here we report that the commonly used stabilized S ectodomain construct “2P” is sensitive to cold temperature, and this cold sensitivity is abrogated in a “down” state–stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use SARS-CoV-2 S ectodomain. |
| format | Online Article Text |
| id | pubmed-7878407 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-78784072021-07-05 Cold sensitivity of the SARS-CoV-2 spike ectodomain Edwards, Robert J Mansouri, Katayoun Stalls, Victoria Manne, Kartik Watts, Brian Parks, Rob Janowska, Katarzyna Gobeil, Sophie M. C. Kopp, Megan Li, Dapeng Lu, Xiaozhi Mu, Zekun Deyton, Margaret Oguin, Thomas H Sprenz, Jordan Williams, Wilton Saunders, Kevin O. Montefiori, David Sempowski, Gregory D. Henderson, Rory Alam, S. Munir Haynes, Barton F. Acharya, Priyamvada Nat Struct Mol Biol Article The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations, receptor-accessible “up” or receptor-inaccessible “down” states. Here we report that the commonly used stabilized S ectodomain construct “2P” is sensitive to cold temperature, and this cold sensitivity is abrogated in a “down” state–stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use SARS-CoV-2 S ectodomain. 2021-01-05 2021-02 /pmc/articles/PMC7878407/ /pubmed/33402708 http://dx.doi.org/10.1038/s41594-020-00547-5 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Edwards, Robert J Mansouri, Katayoun Stalls, Victoria Manne, Kartik Watts, Brian Parks, Rob Janowska, Katarzyna Gobeil, Sophie M. C. Kopp, Megan Li, Dapeng Lu, Xiaozhi Mu, Zekun Deyton, Margaret Oguin, Thomas H Sprenz, Jordan Williams, Wilton Saunders, Kevin O. Montefiori, David Sempowski, Gregory D. Henderson, Rory Alam, S. Munir Haynes, Barton F. Acharya, Priyamvada Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title | Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title_full | Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title_fullStr | Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title_full_unstemmed | Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title_short | Cold sensitivity of the SARS-CoV-2 spike ectodomain |
| title_sort | cold sensitivity of the sars-cov-2 spike ectodomain |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878407/ https://www.ncbi.nlm.nih.gov/pubmed/33402708 http://dx.doi.org/10.1038/s41594-020-00547-5 |
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