Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR

Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were ident...

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Detalles Bibliográficos
Autores principales: Yao, Qiumei, Bai, Yinlei, Kumar, Shaji, Au, Elaine, Orfao, Alberto, Chim, Chor Sang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878533/
https://www.ncbi.nlm.nih.gov/pubmed/33585233
http://dx.doi.org/10.3389/fonc.2020.611021
Descripción
Sumario:Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10(−5) was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10(−5) in 7 (24%), 5 × 10(−5) in 15 (52%), and 10(−4) in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included.

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