Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR

Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were ident...

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Autores principales: Yao, Qiumei, Bai, Yinlei, Kumar, Shaji, Au, Elaine, Orfao, Alberto, Chim, Chor Sang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878533/
https://www.ncbi.nlm.nih.gov/pubmed/33585233
http://dx.doi.org/10.3389/fonc.2020.611021
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author Yao, Qiumei
Bai, Yinlei
Kumar, Shaji
Au, Elaine
Orfao, Alberto
Chim, Chor Sang
author_facet Yao, Qiumei
Bai, Yinlei
Kumar, Shaji
Au, Elaine
Orfao, Alberto
Chim, Chor Sang
author_sort Yao, Qiumei
collection PubMed
description Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10(−5) was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10(−5) in 7 (24%), 5 × 10(−5) in 15 (52%), and 10(−4) in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included.
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spelling pubmed-78785332021-02-13 Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR Yao, Qiumei Bai, Yinlei Kumar, Shaji Au, Elaine Orfao, Alberto Chim, Chor Sang Front Oncol Oncology Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10(−5) was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10(−5) in 7 (24%), 5 × 10(−5) in 15 (52%), and 10(−4) in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included. Frontiers Media S.A. 2021-01-29 /pmc/articles/PMC7878533/ /pubmed/33585233 http://dx.doi.org/10.3389/fonc.2020.611021 Text en Copyright © 2021 Yao, Bai, Kumar, Au, Orfao and Chim http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Yao, Qiumei
Bai, Yinlei
Kumar, Shaji
Au, Elaine
Orfao, Alberto
Chim, Chor Sang
Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title_full Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title_fullStr Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title_full_unstemmed Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title_short Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR
title_sort minimal residual disease detection by next-generation sequencing in multiple myeloma: a comparison with real-time quantitative pcr
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878533/
https://www.ncbi.nlm.nih.gov/pubmed/33585233
http://dx.doi.org/10.3389/fonc.2020.611021
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