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Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanosco...

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Autores principales: Trofymchuk, Kateryna, Glembockyte, Viktorija, Grabenhorst, Lennart, Steiner, Florian, Vietz, Carolin, Close, Cindy, Pfeiffer, Martina, Richter, Lars, Schütte, Max L., Selbach, Florian, Yaadav, Renukka, Zähringer, Jonas, Wei, Qingshan, Ozcan, Aydogan, Lalkens, Birka, Acuna, Guillermo P., Tinnefeld, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878865/
https://www.ncbi.nlm.nih.gov/pubmed/33574261
http://dx.doi.org/10.1038/s41467-021-21238-9
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author Trofymchuk, Kateryna
Glembockyte, Viktorija
Grabenhorst, Lennart
Steiner, Florian
Vietz, Carolin
Close, Cindy
Pfeiffer, Martina
Richter, Lars
Schütte, Max L.
Selbach, Florian
Yaadav, Renukka
Zähringer, Jonas
Wei, Qingshan
Ozcan, Aydogan
Lalkens, Birka
Acuna, Guillermo P.
Tinnefeld, Philip
author_facet Trofymchuk, Kateryna
Glembockyte, Viktorija
Grabenhorst, Lennart
Steiner, Florian
Vietz, Carolin
Close, Cindy
Pfeiffer, Martina
Richter, Lars
Schütte, Max L.
Selbach, Florian
Yaadav, Renukka
Zähringer, Jonas
Wei, Qingshan
Ozcan, Aydogan
Lalkens, Birka
Acuna, Guillermo P.
Tinnefeld, Philip
author_sort Trofymchuk, Kateryna
collection PubMed
description The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.
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spelling pubmed-78788652021-02-24 Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope Trofymchuk, Kateryna Glembockyte, Viktorija Grabenhorst, Lennart Steiner, Florian Vietz, Carolin Close, Cindy Pfeiffer, Martina Richter, Lars Schütte, Max L. Selbach, Florian Yaadav, Renukka Zähringer, Jonas Wei, Qingshan Ozcan, Aydogan Lalkens, Birka Acuna, Guillermo P. Tinnefeld, Philip Nat Commun Article The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road. Nature Publishing Group UK 2021-02-11 /pmc/articles/PMC7878865/ /pubmed/33574261 http://dx.doi.org/10.1038/s41467-021-21238-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Trofymchuk, Kateryna
Glembockyte, Viktorija
Grabenhorst, Lennart
Steiner, Florian
Vietz, Carolin
Close, Cindy
Pfeiffer, Martina
Richter, Lars
Schütte, Max L.
Selbach, Florian
Yaadav, Renukka
Zähringer, Jonas
Wei, Qingshan
Ozcan, Aydogan
Lalkens, Birka
Acuna, Guillermo P.
Tinnefeld, Philip
Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title_full Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title_fullStr Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title_full_unstemmed Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title_short Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
title_sort addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878865/
https://www.ncbi.nlm.nih.gov/pubmed/33574261
http://dx.doi.org/10.1038/s41467-021-21238-9
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