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Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanosco...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878865/ https://www.ncbi.nlm.nih.gov/pubmed/33574261 http://dx.doi.org/10.1038/s41467-021-21238-9 |
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author | Trofymchuk, Kateryna Glembockyte, Viktorija Grabenhorst, Lennart Steiner, Florian Vietz, Carolin Close, Cindy Pfeiffer, Martina Richter, Lars Schütte, Max L. Selbach, Florian Yaadav, Renukka Zähringer, Jonas Wei, Qingshan Ozcan, Aydogan Lalkens, Birka Acuna, Guillermo P. Tinnefeld, Philip |
author_facet | Trofymchuk, Kateryna Glembockyte, Viktorija Grabenhorst, Lennart Steiner, Florian Vietz, Carolin Close, Cindy Pfeiffer, Martina Richter, Lars Schütte, Max L. Selbach, Florian Yaadav, Renukka Zähringer, Jonas Wei, Qingshan Ozcan, Aydogan Lalkens, Birka Acuna, Guillermo P. Tinnefeld, Philip |
author_sort | Trofymchuk, Kateryna |
collection | PubMed |
description | The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road. |
format | Online Article Text |
id | pubmed-7878865 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78788652021-02-24 Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope Trofymchuk, Kateryna Glembockyte, Viktorija Grabenhorst, Lennart Steiner, Florian Vietz, Carolin Close, Cindy Pfeiffer, Martina Richter, Lars Schütte, Max L. Selbach, Florian Yaadav, Renukka Zähringer, Jonas Wei, Qingshan Ozcan, Aydogan Lalkens, Birka Acuna, Guillermo P. Tinnefeld, Philip Nat Commun Article The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road. Nature Publishing Group UK 2021-02-11 /pmc/articles/PMC7878865/ /pubmed/33574261 http://dx.doi.org/10.1038/s41467-021-21238-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Trofymchuk, Kateryna Glembockyte, Viktorija Grabenhorst, Lennart Steiner, Florian Vietz, Carolin Close, Cindy Pfeiffer, Martina Richter, Lars Schütte, Max L. Selbach, Florian Yaadav, Renukka Zähringer, Jonas Wei, Qingshan Ozcan, Aydogan Lalkens, Birka Acuna, Guillermo P. Tinnefeld, Philip Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title | Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title_full | Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title_fullStr | Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title_full_unstemmed | Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title_short | Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
title_sort | addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878865/ https://www.ncbi.nlm.nih.gov/pubmed/33574261 http://dx.doi.org/10.1038/s41467-021-21238-9 |
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