Cargando…

Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms

PURPOSE: To evaluate the treatment effect of radiofrequency-induced hyperthermia (RFH) combined with intra-tumoral chemotherapy for rabbit VX2 liver tumors and explore the underlying mechanism that drives local hyperthermia-enhanced chemotherapy. MATERIALS AND METHODS: VX2 cell lines and rabbits wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Qian, Kun, Chen, Minjiang, Zhang, Feng, Chick, Jeffrey Forris Beecham, Ji, Hongxiu, Zheng, Chuansheng, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878973/
https://www.ncbi.nlm.nih.gov/pubmed/33585231
http://dx.doi.org/10.3389/fonc.2020.610543
_version_ 1783650436152033280
author Qian, Kun
Chen, Minjiang
Zhang, Feng
Chick, Jeffrey Forris Beecham
Ji, Hongxiu
Zheng, Chuansheng
Yang, Xiaoming
author_facet Qian, Kun
Chen, Minjiang
Zhang, Feng
Chick, Jeffrey Forris Beecham
Ji, Hongxiu
Zheng, Chuansheng
Yang, Xiaoming
author_sort Qian, Kun
collection PubMed
description PURPOSE: To evaluate the treatment effect of radiofrequency-induced hyperthermia (RFH) combined with intra-tumoral chemotherapy for rabbit VX2 liver tumors and explore the underlying mechanism that drives local hyperthermia-enhanced chemotherapy. MATERIALS AND METHODS: VX2 cell lines and rabbits with liver VX2 tumors were randomly allocated to four treatment groups including: (1) combination therapy of Doxorubicin (DOX) plus hyperthermia/RFH (n=6); (2) DOX only; (3) hyperthermia/RFH only (n=6); and (4) phosphate-buffered saline-treated control (n=6). Cell viability and doxorubicin uptake by VX2 tumor cells were assayed using flow cytometry and fluorescence microscopy 24 h after treatments. Western blot was used to evaluate the expression level of heat shock protein 70 (HSP70) in tumor cells and tissues. For the harvested VX2 tumors, fluorescence microscopy was used to evaluate the distribution and penetration of doxorubicin in tumor tissues and HSP70 expression was analyzed by Western blot and immunohistochemistry. RESULTS: RFH enhanced the chemotherapeutic effect of doxorubicin in VX2 cells and rabbit liver VX2 tumors resulting in higher apoptosis and lower cell viability. Flowcytometry of VX2 cells showed more apoptotic cells in combination therapy of hyperthermia and DOX, compared with other three groups in-vitro experiments (45.80 ± 1.27% vs 20.66 ± 0.71%, vs 15.16 ± 0.81% and 0.62 ± 0.06%, respectively, p<0.01). The quantitative analysis by Western blot and immunohistochemistry showed increased expression of HSP70 in both VX2 tumor cells (1.28 ± 0.13 vs 0.64 ± 0.13 vs 0.83 ± 0.10 vs 0.15 ± 0.03, respectively, p<0.05) and tumors (1.47 ± 0.13 vs 0.51 ± 0.13 vs 0.74 ± 0.11 vs 0.16 ± 0.04, respectively, p <0.01). Fluorescence microscopy showed increased uptake of DOX in tumor cells in the combination therapy group. CONCLUSIONS: RFH/hyperthermia enhanced the chemotherapeutic effect of DOX in VX2 tumors by promoting the uptake of DOX and the expression HSP70 in tumors.
format Online
Article
Text
id pubmed-7878973
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78789732021-02-13 Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms Qian, Kun Chen, Minjiang Zhang, Feng Chick, Jeffrey Forris Beecham Ji, Hongxiu Zheng, Chuansheng Yang, Xiaoming Front Oncol Oncology PURPOSE: To evaluate the treatment effect of radiofrequency-induced hyperthermia (RFH) combined with intra-tumoral chemotherapy for rabbit VX2 liver tumors and explore the underlying mechanism that drives local hyperthermia-enhanced chemotherapy. MATERIALS AND METHODS: VX2 cell lines and rabbits with liver VX2 tumors were randomly allocated to four treatment groups including: (1) combination therapy of Doxorubicin (DOX) plus hyperthermia/RFH (n=6); (2) DOX only; (3) hyperthermia/RFH only (n=6); and (4) phosphate-buffered saline-treated control (n=6). Cell viability and doxorubicin uptake by VX2 tumor cells were assayed using flow cytometry and fluorescence microscopy 24 h after treatments. Western blot was used to evaluate the expression level of heat shock protein 70 (HSP70) in tumor cells and tissues. For the harvested VX2 tumors, fluorescence microscopy was used to evaluate the distribution and penetration of doxorubicin in tumor tissues and HSP70 expression was analyzed by Western blot and immunohistochemistry. RESULTS: RFH enhanced the chemotherapeutic effect of doxorubicin in VX2 cells and rabbit liver VX2 tumors resulting in higher apoptosis and lower cell viability. Flowcytometry of VX2 cells showed more apoptotic cells in combination therapy of hyperthermia and DOX, compared with other three groups in-vitro experiments (45.80 ± 1.27% vs 20.66 ± 0.71%, vs 15.16 ± 0.81% and 0.62 ± 0.06%, respectively, p<0.01). The quantitative analysis by Western blot and immunohistochemistry showed increased expression of HSP70 in both VX2 tumor cells (1.28 ± 0.13 vs 0.64 ± 0.13 vs 0.83 ± 0.10 vs 0.15 ± 0.03, respectively, p<0.05) and tumors (1.47 ± 0.13 vs 0.51 ± 0.13 vs 0.74 ± 0.11 vs 0.16 ± 0.04, respectively, p <0.01). Fluorescence microscopy showed increased uptake of DOX in tumor cells in the combination therapy group. CONCLUSIONS: RFH/hyperthermia enhanced the chemotherapeutic effect of DOX in VX2 tumors by promoting the uptake of DOX and the expression HSP70 in tumors. Frontiers Media S.A. 2021-01-28 /pmc/articles/PMC7878973/ /pubmed/33585231 http://dx.doi.org/10.3389/fonc.2020.610543 Text en Copyright © 2021 Qian, Chen, Zhang, Chick, Ji, Zheng and Yang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Qian, Kun
Chen, Minjiang
Zhang, Feng
Chick, Jeffrey Forris Beecham
Ji, Hongxiu
Zheng, Chuansheng
Yang, Xiaoming
Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title_full Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title_fullStr Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title_full_unstemmed Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title_short Image-Guided Radiofrequency Hyperthermia (RFH)-Enhanced Direct Chemotherapy of Hepatic Tumors: The Underlying Biomolecular Mechanisms
title_sort image-guided radiofrequency hyperthermia (rfh)-enhanced direct chemotherapy of hepatic tumors: the underlying biomolecular mechanisms
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878973/
https://www.ncbi.nlm.nih.gov/pubmed/33585231
http://dx.doi.org/10.3389/fonc.2020.610543
work_keys_str_mv AT qiankun imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT chenminjiang imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT zhangfeng imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT chickjeffreyforrisbeecham imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT jihongxiu imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT zhengchuansheng imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms
AT yangxiaoming imageguidedradiofrequencyhyperthermiarfhenhanceddirectchemotherapyofhepatictumorstheunderlyingbiomolecularmechanisms