Cargando…
Bioorthogonal mRNA labeling at the poly(A) tail for imaging localization and dynamics in live zebrafish embryos
Live imaging of mRNA in cells and organisms is important for understanding the dynamic aspects underlying its function. Ideally, labeling of mRNA should not alter its structure or function, nor affect the biological system. However, most methods applied in vivo make use of genetically encoded tags a...
Autores principales: | Westerich, Kim J., Chandrasekaran, Karthik S., Gross-Thebing, Theresa, Kueck, Nadine, Raz, Erez, Rentmeister, Andrea |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879197/ https://www.ncbi.nlm.nih.gov/pubmed/33623655 http://dx.doi.org/10.1039/c9sc05981d |
Ejemplares similares
-
Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos
por: Gross-Thebing, Theresa, et al.
Publicado: (2014) -
Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1
por: Westerich, Kim Joana, et al.
Publicado: (2023) -
Multiple covalent fluorescence labeling of eukaryotic mRNA at the poly(A) tail enhances translation and can be performed in living cells
por: Anhäuser, Lea, et al.
Publicado: (2019) -
MePMe-seq: antibody-free simultaneous m(6)A and m(5)C mapping in mRNA by metabolic propargyl labeling and sequencing
por: Hartstock, Katja, et al.
Publicado: (2023) -
Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition
por: Liu, Yusheng, et al.
Publicado: (2023)