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Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces

In multicellular eukaryotes, RNA polymerase (Pol) II pauses transcription ~30–50 bp after initiation. While the budding yeast Saccharomyces has its transcription mechanisms mostly conserved with other eukaryotes, it appears to lack this fundamental promoter-proximal pausing. However, we now report t...

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Autores principales: Badjatia, Nitika, Rossi, Matthew J., Bataille, Alain R., Mittal, Chitvan, Lai, William K.M., Pugh, B. Franklin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879390/
https://www.ncbi.nlm.nih.gov/pubmed/33472084
http://dx.doi.org/10.1016/j.celrep.2020.108640
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author Badjatia, Nitika
Rossi, Matthew J.
Bataille, Alain R.
Mittal, Chitvan
Lai, William K.M.
Pugh, B. Franklin
author_facet Badjatia, Nitika
Rossi, Matthew J.
Bataille, Alain R.
Mittal, Chitvan
Lai, William K.M.
Pugh, B. Franklin
author_sort Badjatia, Nitika
collection PubMed
description In multicellular eukaryotes, RNA polymerase (Pol) II pauses transcription ~30–50 bp after initiation. While the budding yeast Saccharomyces has its transcription mechanisms mostly conserved with other eukaryotes, it appears to lack this fundamental promoter-proximal pausing. However, we now report that nearly all yeast genes, including constitutive and inducible genes, manifest two distinct transcriptional stall sites that are brought on by acute environmental signaling (e.g., peroxide stress). Pol II first stalls at the pre-initiation stage before promoter clearance, but after DNA melting and factor acquisition, and may involve inhibited dephosphorylation. The second stall occurs at the +2 nucleosome. It acquires most, but not all, elongation factor interactions. Its regulation may include Bur1/Spt4/5. Our results suggest that a double Pol II stall is a mechanism to downregulate essentially all genes in concert.
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spelling pubmed-78793902021-02-12 Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces Badjatia, Nitika Rossi, Matthew J. Bataille, Alain R. Mittal, Chitvan Lai, William K.M. Pugh, B. Franklin Cell Rep Article In multicellular eukaryotes, RNA polymerase (Pol) II pauses transcription ~30–50 bp after initiation. While the budding yeast Saccharomyces has its transcription mechanisms mostly conserved with other eukaryotes, it appears to lack this fundamental promoter-proximal pausing. However, we now report that nearly all yeast genes, including constitutive and inducible genes, manifest two distinct transcriptional stall sites that are brought on by acute environmental signaling (e.g., peroxide stress). Pol II first stalls at the pre-initiation stage before promoter clearance, but after DNA melting and factor acquisition, and may involve inhibited dephosphorylation. The second stall occurs at the +2 nucleosome. It acquires most, but not all, elongation factor interactions. Its regulation may include Bur1/Spt4/5. Our results suggest that a double Pol II stall is a mechanism to downregulate essentially all genes in concert. 2021-01-19 /pmc/articles/PMC7879390/ /pubmed/33472084 http://dx.doi.org/10.1016/j.celrep.2020.108640 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Badjatia, Nitika
Rossi, Matthew J.
Bataille, Alain R.
Mittal, Chitvan
Lai, William K.M.
Pugh, B. Franklin
Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title_full Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title_fullStr Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title_full_unstemmed Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title_short Acute stress drives global repression through two independent RNA polymerase II stalling events in Saccharomyces
title_sort acute stress drives global repression through two independent rna polymerase ii stalling events in saccharomyces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879390/
https://www.ncbi.nlm.nih.gov/pubmed/33472084
http://dx.doi.org/10.1016/j.celrep.2020.108640
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