Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020

We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if C(t) values across platforms are comparable. We also investigate whether a C(t) value, a surrogate for calculated viral loads in the absence of viral c...

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Detalles Bibliográficos
Autores principales: Carroll, Anne, McNamara, Eleanor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2021
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879503/
https://www.ncbi.nlm.nih.gov/pubmed/33573710
http://dx.doi.org/10.2807/1560-7917.ES.2021.26.6.2002079
Descripción
Sumario:We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if C(t) values across platforms are comparable. We also investigate whether a C(t) value, a surrogate for calculated viral loads in the absence of viral culture of > 34 can be used to exclude SARS-CoV-2 infection and its complications. We found a variation in C(t )values from different assays for the same calculated viral load; this should be taken into consideration for result interpretation.

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