Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020

We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if C(t) values across platforms are comparable. We also investigate whether a C(t) value, a surrogate for calculated viral loads in the absence of viral c...

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Detalles Bibliográficos
Autores principales: Carroll, Anne, McNamara, Eleanor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2021
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879503/
https://www.ncbi.nlm.nih.gov/pubmed/33573710
http://dx.doi.org/10.2807/1560-7917.ES.2021.26.6.2002079
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author Carroll, Anne
McNamara, Eleanor
author_facet Carroll, Anne
McNamara, Eleanor
author_sort Carroll, Anne
collection PubMed
description We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if C(t) values across platforms are comparable. We also investigate whether a C(t) value, a surrogate for calculated viral loads in the absence of viral culture of > 34 can be used to exclude SARS-CoV-2 infection and its complications. We found a variation in C(t )values from different assays for the same calculated viral load; this should be taken into consideration for result interpretation.
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spelling pubmed-78795032021-02-23 Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020 Carroll, Anne McNamara, Eleanor Euro Surveill Rapid Communication We report the performance of a variety of commercially available SARS-CoV-2 PCR kits, used in several different sites across Ireland to determine if C(t) values across platforms are comparable. We also investigate whether a C(t) value, a surrogate for calculated viral loads in the absence of viral culture of > 34 can be used to exclude SARS-CoV-2 infection and its complications. We found a variation in C(t )values from different assays for the same calculated viral load; this should be taken into consideration for result interpretation. European Centre for Disease Prevention and Control (ECDC) 2021-02-11 /pmc/articles/PMC7879503/ /pubmed/33573710 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.6.2002079 Text en This article is copyright of the authors or their affiliated institutions, 2021. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Rapid Communication
Carroll, Anne
McNamara, Eleanor
Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title_full Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title_fullStr Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title_full_unstemmed Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title_short Comparison and correlation of commercial SARS-CoV-2 real-time-PCR assays, Ireland, June 2020
title_sort comparison and correlation of commercial sars-cov-2 real-time-pcr assays, ireland, june 2020
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879503/
https://www.ncbi.nlm.nih.gov/pubmed/33573710
http://dx.doi.org/10.2807/1560-7917.ES.2021.26.6.2002079
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