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Rules governing genetic exchanges among viral types from different Enterovirus A clusters

The species Enterovirus A (EV-A) consists of two conventional clusters and one unconventional cluster. At present, sequence analysis shows no evidence of recombination between conventional and unconventional EV-A types. However, the factors underlying this genetic barrier are unclear. Here, we syste...

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Autores principales: Wang, Min, Zhu, Liuyao, Fan, Jun, Yan, Jingjing, Dun, Ying, Yu, Rui, Liu, Lizhen, Zhang, Shuye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879560/
https://www.ncbi.nlm.nih.gov/pubmed/32762804
http://dx.doi.org/10.1099/jgv.0.001479
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author Wang, Min
Zhu, Liuyao
Fan, Jun
Yan, Jingjing
Dun, Ying
Yu, Rui
Liu, Lizhen
Zhang, Shuye
author_facet Wang, Min
Zhu, Liuyao
Fan, Jun
Yan, Jingjing
Dun, Ying
Yu, Rui
Liu, Lizhen
Zhang, Shuye
author_sort Wang, Min
collection PubMed
description The species Enterovirus A (EV-A) consists of two conventional clusters and one unconventional cluster. At present, sequence analysis shows no evidence of recombination between conventional and unconventional EV-A types. However, the factors underlying this genetic barrier are unclear. Here, we systematically dissected the genome components linked to these peculiar phenomena, using the viral reverse genetic tools. We reported that viral capsids of the unconventional EV-A types expressed poorly in human cells. The trans-encapsidation outputs across conventional and unconventional EV-A types were also with low efficiency. However, replicons of conventional types bearing exchanged 5′-untranslated region (UTR) or non-structural regions from the unconventional types were replication-competent. Furthermore, we created a viable recombinant EVA71 (conventional type) with its P3 region replaced by that from EVA89 (unconventional type). Thus, our data for the first time reveal the potential for fertile genetic exchanges between conventional and unconventional EV-A types. It also discloses that the mysterious recombination barriers may lie in uncoordinated capsid expression and particle assembly by different EV-A clusters.
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spelling pubmed-78795602021-02-12 Rules governing genetic exchanges among viral types from different Enterovirus A clusters Wang, Min Zhu, Liuyao Fan, Jun Yan, Jingjing Dun, Ying Yu, Rui Liu, Lizhen Zhang, Shuye J Gen Virol Research Article The species Enterovirus A (EV-A) consists of two conventional clusters and one unconventional cluster. At present, sequence analysis shows no evidence of recombination between conventional and unconventional EV-A types. However, the factors underlying this genetic barrier are unclear. Here, we systematically dissected the genome components linked to these peculiar phenomena, using the viral reverse genetic tools. We reported that viral capsids of the unconventional EV-A types expressed poorly in human cells. The trans-encapsidation outputs across conventional and unconventional EV-A types were also with low efficiency. However, replicons of conventional types bearing exchanged 5′-untranslated region (UTR) or non-structural regions from the unconventional types were replication-competent. Furthermore, we created a viable recombinant EVA71 (conventional type) with its P3 region replaced by that from EVA89 (unconventional type). Thus, our data for the first time reveal the potential for fertile genetic exchanges between conventional and unconventional EV-A types. It also discloses that the mysterious recombination barriers may lie in uncoordinated capsid expression and particle assembly by different EV-A clusters. Microbiology Society 2020-11 2020-08-07 /pmc/articles/PMC7879560/ /pubmed/32762804 http://dx.doi.org/10.1099/jgv.0.001479 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Research Article
Wang, Min
Zhu, Liuyao
Fan, Jun
Yan, Jingjing
Dun, Ying
Yu, Rui
Liu, Lizhen
Zhang, Shuye
Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title_full Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title_fullStr Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title_full_unstemmed Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title_short Rules governing genetic exchanges among viral types from different Enterovirus A clusters
title_sort rules governing genetic exchanges among viral types from different enterovirus a clusters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879560/
https://www.ncbi.nlm.nih.gov/pubmed/32762804
http://dx.doi.org/10.1099/jgv.0.001479
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