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Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879953/ https://www.ncbi.nlm.nih.gov/pubmed/33614261 http://dx.doi.org/10.7717/peerj.10580 |
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author | Lin, Qiujun Gao, Yue Wu, Xianxin Ni, Xinyu Chen, Rongzhen Xuan, Yuanhu Li, Tianya |
author_facet | Lin, Qiujun Gao, Yue Wu, Xianxin Ni, Xinyu Chen, Rongzhen Xuan, Yuanhu Li, Tianya |
author_sort | Lin, Qiujun |
collection | PubMed |
description | Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spread of the Ug99 race variants. Therefore, there is an urgent global effort to identify new germplasm resources effective against those races. In this study, the resistance to Pgt of 95 wheat advance lines from Heilongjiang Province was evaluated using three predominant races of Pgt, 21C3CTTTM, 34C0MKGSM, and 34C3MTGQM, in China at the seedling and adult plant stage. The presence of 6 Sr genes (Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38) was evaluated using linked molecular markers. The results showed that 86 (90.5%) wheat lines had plant stage resistance to all three races. Molecular marker analysis showed that 24 wheat lines likely carried Sr38, 15 wheat lines likely carried Sr2, 11 wheat lines likely carried Sr31, while none of the wheat lines carried Sr24, Sr25, or Sr26. Furthermore, six out of the 95 wheat lines tested carried both Sr2 and Sr38, three contained both Sr31 and Sr38, and two wheat lines contained both Sr2 and Sr31. Wheat lines with known Sr genes may be used as donor parents for further breeding programs to provide resistance to stem rust. |
format | Online Article Text |
id | pubmed-7879953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78799532021-02-18 Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province Lin, Qiujun Gao, Yue Wu, Xianxin Ni, Xinyu Chen, Rongzhen Xuan, Yuanhu Li, Tianya PeerJ Agricultural Science Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spread of the Ug99 race variants. Therefore, there is an urgent global effort to identify new germplasm resources effective against those races. In this study, the resistance to Pgt of 95 wheat advance lines from Heilongjiang Province was evaluated using three predominant races of Pgt, 21C3CTTTM, 34C0MKGSM, and 34C3MTGQM, in China at the seedling and adult plant stage. The presence of 6 Sr genes (Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38) was evaluated using linked molecular markers. The results showed that 86 (90.5%) wheat lines had plant stage resistance to all three races. Molecular marker analysis showed that 24 wheat lines likely carried Sr38, 15 wheat lines likely carried Sr2, 11 wheat lines likely carried Sr31, while none of the wheat lines carried Sr24, Sr25, or Sr26. Furthermore, six out of the 95 wheat lines tested carried both Sr2 and Sr38, three contained both Sr31 and Sr38, and two wheat lines contained both Sr2 and Sr31. Wheat lines with known Sr genes may be used as donor parents for further breeding programs to provide resistance to stem rust. PeerJ Inc. 2021-02-09 /pmc/articles/PMC7879953/ /pubmed/33614261 http://dx.doi.org/10.7717/peerj.10580 Text en ©2021 Lin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Lin, Qiujun Gao, Yue Wu, Xianxin Ni, Xinyu Chen, Rongzhen Xuan, Yuanhu Li, Tianya Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title | Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title_full | Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title_fullStr | Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title_full_unstemmed | Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title_short | Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province |
title_sort | evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from heilongjiang province |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879953/ https://www.ncbi.nlm.nih.gov/pubmed/33614261 http://dx.doi.org/10.7717/peerj.10580 |
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