Cargando…

Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spr...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Qiujun, Gao, Yue, Wu, Xianxin, Ni, Xinyu, Chen, Rongzhen, Xuan, Yuanhu, Li, Tianya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879953/
https://www.ncbi.nlm.nih.gov/pubmed/33614261
http://dx.doi.org/10.7717/peerj.10580
_version_ 1783650617225379840
author Lin, Qiujun
Gao, Yue
Wu, Xianxin
Ni, Xinyu
Chen, Rongzhen
Xuan, Yuanhu
Li, Tianya
author_facet Lin, Qiujun
Gao, Yue
Wu, Xianxin
Ni, Xinyu
Chen, Rongzhen
Xuan, Yuanhu
Li, Tianya
author_sort Lin, Qiujun
collection PubMed
description Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spread of the Ug99 race variants. Therefore, there is an urgent global effort to identify new germplasm resources effective against those races. In this study, the resistance to Pgt of 95 wheat advance lines from Heilongjiang Province was evaluated using three predominant races of Pgt, 21C3CTTTM, 34C0MKGSM, and 34C3MTGQM, in China at the seedling and adult plant stage. The presence of 6 Sr genes (Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38) was evaluated using linked molecular markers. The results showed that 86 (90.5%) wheat lines had plant stage resistance to all three races. Molecular marker analysis showed that 24 wheat lines likely carried Sr38, 15 wheat lines likely carried Sr2, 11 wheat lines likely carried Sr31, while none of the wheat lines carried Sr24, Sr25, or Sr26. Furthermore, six out of the 95 wheat lines tested carried both Sr2 and Sr38, three contained both Sr31 and Sr38, and two wheat lines contained both Sr2 and Sr31. Wheat lines with known Sr genes may be used as donor parents for further breeding programs to provide resistance to stem rust.
format Online
Article
Text
id pubmed-7879953
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-78799532021-02-18 Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province Lin, Qiujun Gao, Yue Wu, Xianxin Ni, Xinyu Chen, Rongzhen Xuan, Yuanhu Li, Tianya PeerJ Agricultural Science Wheat stem rust, caused by Puccinia graminis f. sp. tritici, (Pgt) is a devastating disease in wheat production. The disease has been effectively controlled since the 1970s due to the widespread use of the Sr31 resistance gene. However, Sr31 has lost its effectiveness following the emergence and spread of the Ug99 race variants. Therefore, there is an urgent global effort to identify new germplasm resources effective against those races. In this study, the resistance to Pgt of 95 wheat advance lines from Heilongjiang Province was evaluated using three predominant races of Pgt, 21C3CTTTM, 34C0MKGSM, and 34C3MTGQM, in China at the seedling and adult plant stage. The presence of 6 Sr genes (Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38) was evaluated using linked molecular markers. The results showed that 86 (90.5%) wheat lines had plant stage resistance to all three races. Molecular marker analysis showed that 24 wheat lines likely carried Sr38, 15 wheat lines likely carried Sr2, 11 wheat lines likely carried Sr31, while none of the wheat lines carried Sr24, Sr25, or Sr26. Furthermore, six out of the 95 wheat lines tested carried both Sr2 and Sr38, three contained both Sr31 and Sr38, and two wheat lines contained both Sr2 and Sr31. Wheat lines with known Sr genes may be used as donor parents for further breeding programs to provide resistance to stem rust. PeerJ Inc. 2021-02-09 /pmc/articles/PMC7879953/ /pubmed/33614261 http://dx.doi.org/10.7717/peerj.10580 Text en ©2021 Lin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Lin, Qiujun
Gao, Yue
Wu, Xianxin
Ni, Xinyu
Chen, Rongzhen
Xuan, Yuanhu
Li, Tianya
Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title_full Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title_fullStr Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title_full_unstemmed Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title_short Evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from Heilongjiang province
title_sort evaluation of resistance to wheat stem rust and identification of resistance genes in wheat lines from heilongjiang province
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879953/
https://www.ncbi.nlm.nih.gov/pubmed/33614261
http://dx.doi.org/10.7717/peerj.10580
work_keys_str_mv AT linqiujun evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT gaoyue evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT wuxianxin evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT nixinyu evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT chenrongzhen evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT xuanyuanhu evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince
AT litianya evaluationofresistancetowheatstemrustandidentificationofresistancegenesinwheatlinesfromheilongjiangprovince