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Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing
BACKGROUND: In China, double-seed (DS) sowing (i.e., sowing two seeds per hole) has been conventionally performed towards the erect-plant-type peanuts to increase the low germination rate due to poor seed preservation conditions. However, the corresponding within-hole plant competition usually limit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879956/ https://www.ncbi.nlm.nih.gov/pubmed/33614263 http://dx.doi.org/10.7717/peerj.10616 |
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author | Yang, Sha Zhang, Jialei Geng, Yun Tang, Zhaohui Wang, Jianguo Guo, Feng Meng, Jingjing Wang, Quan Wan, Shubo Li, Xinguo |
author_facet | Yang, Sha Zhang, Jialei Geng, Yun Tang, Zhaohui Wang, Jianguo Guo, Feng Meng, Jingjing Wang, Quan Wan, Shubo Li, Xinguo |
author_sort | Yang, Sha |
collection | PubMed |
description | BACKGROUND: In China, double-seed (DS) sowing (i.e., sowing two seeds per hole) has been conventionally performed towards the erect-plant-type peanuts to increase the low germination rate due to poor seed preservation conditions. However, the corresponding within-hole plant competition usually limits the subsequent plant growth and the final yield. We developed a high-yield cultivation system of single-seed (SS) precision sowing to solve this paradox, saving 20% of seeds and increasing yields by more than 10% relative to the conventional DS sowing. METHODS: To explore the mechanisms of these two different cropping patterns in peanut yields, we conducted transcriptomic and physiological comparisons in the seeding plant leaf and root tissues between SS precision sowing and standard DS sowing treatments. RESULTS: After assembly, each library contained an average of 43 million reads and generated a total of 523,800, 338 clean reads. After GO and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found the key genes for biotic and abiotic stress showed higher expression in roots of plants grown under the SS precision sowing treatment, including genes encoding disease resistance, oxidation-reduction, hormone related, and stress response transcription factors and signaling regulation proteins. In particular, the resveratrol synthesis genes related to stress and disease resistance appeared induced in roots under the SS sowing treatment. CONCLUSION: These data indicated that Aspergillus flavus resistance and stress tolerance in roots under SS precision sowing were enhanced compared with roots under the DS sowing treatment. This work benefits the development of underground pods and thus increasing peanut yields. |
format | Online Article Text |
id | pubmed-7879956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78799562021-02-18 Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing Yang, Sha Zhang, Jialei Geng, Yun Tang, Zhaohui Wang, Jianguo Guo, Feng Meng, Jingjing Wang, Quan Wan, Shubo Li, Xinguo PeerJ Agricultural Science BACKGROUND: In China, double-seed (DS) sowing (i.e., sowing two seeds per hole) has been conventionally performed towards the erect-plant-type peanuts to increase the low germination rate due to poor seed preservation conditions. However, the corresponding within-hole plant competition usually limits the subsequent plant growth and the final yield. We developed a high-yield cultivation system of single-seed (SS) precision sowing to solve this paradox, saving 20% of seeds and increasing yields by more than 10% relative to the conventional DS sowing. METHODS: To explore the mechanisms of these two different cropping patterns in peanut yields, we conducted transcriptomic and physiological comparisons in the seeding plant leaf and root tissues between SS precision sowing and standard DS sowing treatments. RESULTS: After assembly, each library contained an average of 43 million reads and generated a total of 523,800, 338 clean reads. After GO and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found the key genes for biotic and abiotic stress showed higher expression in roots of plants grown under the SS precision sowing treatment, including genes encoding disease resistance, oxidation-reduction, hormone related, and stress response transcription factors and signaling regulation proteins. In particular, the resveratrol synthesis genes related to stress and disease resistance appeared induced in roots under the SS sowing treatment. CONCLUSION: These data indicated that Aspergillus flavus resistance and stress tolerance in roots under SS precision sowing were enhanced compared with roots under the DS sowing treatment. This work benefits the development of underground pods and thus increasing peanut yields. PeerJ Inc. 2021-02-09 /pmc/articles/PMC7879956/ /pubmed/33614263 http://dx.doi.org/10.7717/peerj.10616 Text en © 2021 Yang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Yang, Sha Zhang, Jialei Geng, Yun Tang, Zhaohui Wang, Jianguo Guo, Feng Meng, Jingjing Wang, Quan Wan, Shubo Li, Xinguo Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title | Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title_full | Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title_fullStr | Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title_full_unstemmed | Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title_short | Transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
title_sort | transcriptome analysis reveals the mechanism of improving erect-plant-type peanut yield by single-seeding precision sowing |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879956/ https://www.ncbi.nlm.nih.gov/pubmed/33614263 http://dx.doi.org/10.7717/peerj.10616 |
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