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Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades

Circular RNAs (circRNAs) have a critical regulatory function in human glioma. However, novel circRNAs related to different pathological grades of glioma and their crucial potential function are worth screening and prediction. CircRNA expression profiling was performed for 6 paired high- and low-grad...

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Autores principales: Geng, Xiuchao, Zhang, Yuhao, Li, Qiang, Xi, Wang, Yu, Wentao, Shi, Liang, Lin, Xiaomeng, Sun, Shaoguang, Wang, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880344/
https://www.ncbi.nlm.nih.gov/pubmed/33323543
http://dx.doi.org/10.18632/aging.202192
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author Geng, Xiuchao
Zhang, Yuhao
Li, Qiang
Xi, Wang
Yu, Wentao
Shi, Liang
Lin, Xiaomeng
Sun, Shaoguang
Wang, Hong
author_facet Geng, Xiuchao
Zhang, Yuhao
Li, Qiang
Xi, Wang
Yu, Wentao
Shi, Liang
Lin, Xiaomeng
Sun, Shaoguang
Wang, Hong
author_sort Geng, Xiuchao
collection PubMed
description Circular RNAs (circRNAs) have a critical regulatory function in human glioma. However, novel circRNAs related to different pathological grades of glioma and their crucial potential function are worth screening and prediction. CircRNA expression profiling was performed for 6 paired high- and low-grade glioma tissues and 5 adjacent normal brain tissues through next-generation sequencing. Quantitative real-time PCR (qRT-PCR) was conducted to validate circRNA expression. Bioinformatics analysis was performed, and circRNA-miRNA-mRNA networks were constructed. The expression and survival data of miRNAs and target genes were examined by GEPIA, Chinese Glioma Genome Atlas (CGGA), ONCOMINE, and cBioPortal databases. The RNA binding proteins (RBPs), open reading frames (ORFs) and N6-methyladenosine (m(6)A) modifications of the identified circRNAs were also predicted. Through multilevel research screening, 4 circRNAs (hsa_circ_0000915, hsa_circ_0127664, hsa_circ_0008362, and hsa_circ_0001467) were associated with glioma of different pathological grades and could be preferred candidates for subsequent functional analysis. Therefore, circRNAs are associated with the different pathological grades of glioma and reveal their potential critical regulatory function. CircRNAs might provide vital molecular biomarkers and potential therapeutic targets for glioma.
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spelling pubmed-78803442021-02-22 Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades Geng, Xiuchao Zhang, Yuhao Li, Qiang Xi, Wang Yu, Wentao Shi, Liang Lin, Xiaomeng Sun, Shaoguang Wang, Hong Aging (Albany NY) Research Paper Circular RNAs (circRNAs) have a critical regulatory function in human glioma. However, novel circRNAs related to different pathological grades of glioma and their crucial potential function are worth screening and prediction. CircRNA expression profiling was performed for 6 paired high- and low-grade glioma tissues and 5 adjacent normal brain tissues through next-generation sequencing. Quantitative real-time PCR (qRT-PCR) was conducted to validate circRNA expression. Bioinformatics analysis was performed, and circRNA-miRNA-mRNA networks were constructed. The expression and survival data of miRNAs and target genes were examined by GEPIA, Chinese Glioma Genome Atlas (CGGA), ONCOMINE, and cBioPortal databases. The RNA binding proteins (RBPs), open reading frames (ORFs) and N6-methyladenosine (m(6)A) modifications of the identified circRNAs were also predicted. Through multilevel research screening, 4 circRNAs (hsa_circ_0000915, hsa_circ_0127664, hsa_circ_0008362, and hsa_circ_0001467) were associated with glioma of different pathological grades and could be preferred candidates for subsequent functional analysis. Therefore, circRNAs are associated with the different pathological grades of glioma and reveal their potential critical regulatory function. CircRNAs might provide vital molecular biomarkers and potential therapeutic targets for glioma. Impact Journals 2020-12-11 /pmc/articles/PMC7880344/ /pubmed/33323543 http://dx.doi.org/10.18632/aging.202192 Text en Copyright: © 2020 Geng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Geng, Xiuchao
Zhang, Yuhao
Li, Qiang
Xi, Wang
Yu, Wentao
Shi, Liang
Lin, Xiaomeng
Sun, Shaoguang
Wang, Hong
Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title_full Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title_fullStr Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title_full_unstemmed Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title_short Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades
title_sort screening and functional prediction of differentially expressed circular rnas in human glioma of different grades
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880344/
https://www.ncbi.nlm.nih.gov/pubmed/33323543
http://dx.doi.org/10.18632/aging.202192
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