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Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization

Older adults are at increased risk of pneumococcal disease. This work aims to evaluate whether there is any decrease in serum IgG against variants of the antigens Pneumococcal surface protein A (PspA) and Pneumococcal surface protein C (PspC) in healthy adults with increasing age. Levels of IgG agai...

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Autores principales: Araujo, Adriano P., Colichio, Gabriela B. C., Oliveira, Maria Leonor S., German, Esther, Nikolaou, Elissavet, Chen, Tao, Adler, Hugh, Ferreira, Daniela M., Miyaji, Eliane N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880446/
https://www.ncbi.nlm.nih.gov/pubmed/33577617
http://dx.doi.org/10.1371/journal.pone.0247056
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author Araujo, Adriano P.
Colichio, Gabriela B. C.
Oliveira, Maria Leonor S.
German, Esther
Nikolaou, Elissavet
Chen, Tao
Adler, Hugh
Ferreira, Daniela M.
Miyaji, Eliane N.
author_facet Araujo, Adriano P.
Colichio, Gabriela B. C.
Oliveira, Maria Leonor S.
German, Esther
Nikolaou, Elissavet
Chen, Tao
Adler, Hugh
Ferreira, Daniela M.
Miyaji, Eliane N.
author_sort Araujo, Adriano P.
collection PubMed
description Older adults are at increased risk of pneumococcal disease. This work aims to evaluate whether there is any decrease in serum IgG against variants of the antigens Pneumococcal surface protein A (PspA) and Pneumococcal surface protein C (PspC) in healthy adults with increasing age. Levels of IgG against PspA and PspC variants were determined by ELISA in serum samples comparing volunteers 18–30 years of age with volunteers who were 50–70+ before and after an experimental pneumococcal colonization challenge. The serotype 6B strain used in the challenge belongs to a minor group of pneumococcal isolates expressing two PspC variants. There was a decrease in levels of IgG with increasing age for the most common PspA variants and for all PspC variants analyzed. No correlation was found between basal levels of IgG against these antigens and protection against colonization. There was an increase in levels of IgG against PspA variants that are more cross-reactive with the variant expressed by the challenge strain post challenge in younger individuals who became colonized. Since the challenge strain used in our study expresses two different PspC variants, an increase in serum IgG against all PspC variants tested was observed in younger individuals who became colonized. For some of the antigen variants tested, a decrease in serum IgG was observed in young volunteers who were challenged but did not become colonized. Serum IgG antibodies against PspA and PspC variants thus decrease with age in healthy adults, but there is no correlation between levels of IgG against these antigens and protection against human experimental colonization. Though no correlation between naturally induced serum IgG antibodies against PspA and PspC and protection against colonization was observed, these results do not rule out the protective potential of these antigens as vaccines against pneumococcal infections.
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spelling pubmed-78804462021-02-19 Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization Araujo, Adriano P. Colichio, Gabriela B. C. Oliveira, Maria Leonor S. German, Esther Nikolaou, Elissavet Chen, Tao Adler, Hugh Ferreira, Daniela M. Miyaji, Eliane N. PLoS One Research Article Older adults are at increased risk of pneumococcal disease. This work aims to evaluate whether there is any decrease in serum IgG against variants of the antigens Pneumococcal surface protein A (PspA) and Pneumococcal surface protein C (PspC) in healthy adults with increasing age. Levels of IgG against PspA and PspC variants were determined by ELISA in serum samples comparing volunteers 18–30 years of age with volunteers who were 50–70+ before and after an experimental pneumococcal colonization challenge. The serotype 6B strain used in the challenge belongs to a minor group of pneumococcal isolates expressing two PspC variants. There was a decrease in levels of IgG with increasing age for the most common PspA variants and for all PspC variants analyzed. No correlation was found between basal levels of IgG against these antigens and protection against colonization. There was an increase in levels of IgG against PspA variants that are more cross-reactive with the variant expressed by the challenge strain post challenge in younger individuals who became colonized. Since the challenge strain used in our study expresses two different PspC variants, an increase in serum IgG against all PspC variants tested was observed in younger individuals who became colonized. For some of the antigen variants tested, a decrease in serum IgG was observed in young volunteers who were challenged but did not become colonized. Serum IgG antibodies against PspA and PspC variants thus decrease with age in healthy adults, but there is no correlation between levels of IgG against these antigens and protection against human experimental colonization. Though no correlation between naturally induced serum IgG antibodies against PspA and PspC and protection against colonization was observed, these results do not rule out the protective potential of these antigens as vaccines against pneumococcal infections. Public Library of Science 2021-02-12 /pmc/articles/PMC7880446/ /pubmed/33577617 http://dx.doi.org/10.1371/journal.pone.0247056 Text en © 2021 Araujo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Araujo, Adriano P.
Colichio, Gabriela B. C.
Oliveira, Maria Leonor S.
German, Esther
Nikolaou, Elissavet
Chen, Tao
Adler, Hugh
Ferreira, Daniela M.
Miyaji, Eliane N.
Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title_full Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title_fullStr Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title_full_unstemmed Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title_short Serum levels of anti-PspA and anti-PspC IgG decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
title_sort serum levels of anti-pspa and anti-pspc igg decrease with age and do not correlate with susceptibility to experimental human pneumococcal colonization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880446/
https://www.ncbi.nlm.nih.gov/pubmed/33577617
http://dx.doi.org/10.1371/journal.pone.0247056
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