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Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881201/ https://www.ncbi.nlm.nih.gov/pubmed/33580118 http://dx.doi.org/10.1038/s41598-021-83284-z |
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author | Partrick, Katherine A. Rosenhauer, Anna M. Auger, Jérémie Arnold, Amanda R. Ronczkowski, Nicole M. Jackson, Lanaya M. Lord, Magen N. Abdulla, Sara M. Chassaing, Benoit Huhman, Kim L. |
author_facet | Partrick, Katherine A. Rosenhauer, Anna M. Auger, Jérémie Arnold, Amanda R. Ronczkowski, Nicole M. Jackson, Lanaya M. Lord, Magen N. Abdulla, Sara M. Chassaing, Benoit Huhman, Kim L. |
author_sort | Partrick, Katherine A. |
collection | PubMed |
description | Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces the striking increase in anxiety-like behavior and changes in gut microbiota observed following social defeat stress in Syrian hamsters. We administered the probiotic at two different doses for 21 days, and 16S rRNA gene amplicon sequencing revealed a shift in microbial structure following probiotic administration at both doses, independently of stress. Probiotic administration at either dose increased anti-inflammatory cytokines IL-4, IL-5, and IL-10 compared to placebo. Surprisingly, probiotic administration at the low dose, equivalent to the one used in humans, significantly increased social avoidance and decreased social interaction. This behavioral change was associated with a reduction in microbial richness in this group. Together, these results demonstrate that probiotic administration alters gut microbial composition and may promote an anti-inflammatory profile but that these changes may not promote reductions in behavioral responses to social stress. |
format | Online Article Text |
id | pubmed-7881201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-78812012021-02-16 Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress Partrick, Katherine A. Rosenhauer, Anna M. Auger, Jérémie Arnold, Amanda R. Ronczkowski, Nicole M. Jackson, Lanaya M. Lord, Magen N. Abdulla, Sara M. Chassaing, Benoit Huhman, Kim L. Sci Rep Article Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces the striking increase in anxiety-like behavior and changes in gut microbiota observed following social defeat stress in Syrian hamsters. We administered the probiotic at two different doses for 21 days, and 16S rRNA gene amplicon sequencing revealed a shift in microbial structure following probiotic administration at both doses, independently of stress. Probiotic administration at either dose increased anti-inflammatory cytokines IL-4, IL-5, and IL-10 compared to placebo. Surprisingly, probiotic administration at the low dose, equivalent to the one used in humans, significantly increased social avoidance and decreased social interaction. This behavioral change was associated with a reduction in microbial richness in this group. Together, these results demonstrate that probiotic administration alters gut microbial composition and may promote an anti-inflammatory profile but that these changes may not promote reductions in behavioral responses to social stress. Nature Publishing Group UK 2021-02-12 /pmc/articles/PMC7881201/ /pubmed/33580118 http://dx.doi.org/10.1038/s41598-021-83284-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Partrick, Katherine A. Rosenhauer, Anna M. Auger, Jérémie Arnold, Amanda R. Ronczkowski, Nicole M. Jackson, Lanaya M. Lord, Magen N. Abdulla, Sara M. Chassaing, Benoit Huhman, Kim L. Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title | Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title_full | Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title_fullStr | Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title_full_unstemmed | Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title_short | Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
title_sort | ingestion of probiotic (lactobacillus helveticus and bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881201/ https://www.ncbi.nlm.nih.gov/pubmed/33580118 http://dx.doi.org/10.1038/s41598-021-83284-z |
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