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Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress

Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus...

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Autores principales: Partrick, Katherine A., Rosenhauer, Anna M., Auger, Jérémie, Arnold, Amanda R., Ronczkowski, Nicole M., Jackson, Lanaya M., Lord, Magen N., Abdulla, Sara M., Chassaing, Benoit, Huhman, Kim L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881201/
https://www.ncbi.nlm.nih.gov/pubmed/33580118
http://dx.doi.org/10.1038/s41598-021-83284-z
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author Partrick, Katherine A.
Rosenhauer, Anna M.
Auger, Jérémie
Arnold, Amanda R.
Ronczkowski, Nicole M.
Jackson, Lanaya M.
Lord, Magen N.
Abdulla, Sara M.
Chassaing, Benoit
Huhman, Kim L.
author_facet Partrick, Katherine A.
Rosenhauer, Anna M.
Auger, Jérémie
Arnold, Amanda R.
Ronczkowski, Nicole M.
Jackson, Lanaya M.
Lord, Magen N.
Abdulla, Sara M.
Chassaing, Benoit
Huhman, Kim L.
author_sort Partrick, Katherine A.
collection PubMed
description Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces the striking increase in anxiety-like behavior and changes in gut microbiota observed following social defeat stress in Syrian hamsters. We administered the probiotic at two different doses for 21 days, and 16S rRNA gene amplicon sequencing revealed a shift in microbial structure following probiotic administration at both doses, independently of stress. Probiotic administration at either dose increased anti-inflammatory cytokines IL-4, IL-5, and IL-10 compared to placebo. Surprisingly, probiotic administration at the low dose, equivalent to the one used in humans, significantly increased social avoidance and decreased social interaction. This behavioral change was associated with a reduction in microbial richness in this group. Together, these results demonstrate that probiotic administration alters gut microbial composition and may promote an anti-inflammatory profile but that these changes may not promote reductions in behavioral responses to social stress.
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spelling pubmed-78812012021-02-16 Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress Partrick, Katherine A. Rosenhauer, Anna M. Auger, Jérémie Arnold, Amanda R. Ronczkowski, Nicole M. Jackson, Lanaya M. Lord, Magen N. Abdulla, Sara M. Chassaing, Benoit Huhman, Kim L. Sci Rep Article Social stress exacerbates anxious and depressive behaviors in humans. Similarly, anxiety- and depressive-like behaviors are triggered by social stress in a variety of non-human animals. Here, we tested whether oral administration of the putative anxiolytic probiotic strains Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 reduces the striking increase in anxiety-like behavior and changes in gut microbiota observed following social defeat stress in Syrian hamsters. We administered the probiotic at two different doses for 21 days, and 16S rRNA gene amplicon sequencing revealed a shift in microbial structure following probiotic administration at both doses, independently of stress. Probiotic administration at either dose increased anti-inflammatory cytokines IL-4, IL-5, and IL-10 compared to placebo. Surprisingly, probiotic administration at the low dose, equivalent to the one used in humans, significantly increased social avoidance and decreased social interaction. This behavioral change was associated with a reduction in microbial richness in this group. Together, these results demonstrate that probiotic administration alters gut microbial composition and may promote an anti-inflammatory profile but that these changes may not promote reductions in behavioral responses to social stress. Nature Publishing Group UK 2021-02-12 /pmc/articles/PMC7881201/ /pubmed/33580118 http://dx.doi.org/10.1038/s41598-021-83284-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Partrick, Katherine A.
Rosenhauer, Anna M.
Auger, Jérémie
Arnold, Amanda R.
Ronczkowski, Nicole M.
Jackson, Lanaya M.
Lord, Magen N.
Abdulla, Sara M.
Chassaing, Benoit
Huhman, Kim L.
Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title_full Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title_fullStr Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title_full_unstemmed Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title_short Ingestion of probiotic (Lactobacillus helveticus and Bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
title_sort ingestion of probiotic (lactobacillus helveticus and bifidobacterium longum) alters intestinal microbial structure and behavioral expression following social defeat stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881201/
https://www.ncbi.nlm.nih.gov/pubmed/33580118
http://dx.doi.org/10.1038/s41598-021-83284-z
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