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Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging
Carotid atherosclerosis is a risk factor for ischemic stroke, one of the main causes of mortality and disability worldwide. The disease is characterized by plaques, heterogeneous deposits of lipids, and necrotic debris in the vascular wall, which grow gradually and may remain asymptomatic for decade...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881220/ https://www.ncbi.nlm.nih.gov/pubmed/33581415 http://dx.doi.org/10.1194/jlr.RA120000974 |
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author | Moerman, Astrid M. Visscher, Mirjam Slijkhuis, Nuria Van Gaalen, Kim Heijs, Bram Klein, Theo Burgers, Peter C. De Rijke, Yolanda B. Van Beusekom, Heleen M.M. Luider, Theo M. Verhagen, Hence J.M. Van der Steen, Antonius F.W. Gijsen, Frank J.H. Van der Heiden, Kim Van Soest, Gijs |
author_facet | Moerman, Astrid M. Visscher, Mirjam Slijkhuis, Nuria Van Gaalen, Kim Heijs, Bram Klein, Theo Burgers, Peter C. De Rijke, Yolanda B. Van Beusekom, Heleen M.M. Luider, Theo M. Verhagen, Hence J.M. Van der Steen, Antonius F.W. Gijsen, Frank J.H. Van der Heiden, Kim Van Soest, Gijs |
author_sort | Moerman, Astrid M. |
collection | PubMed |
description | Carotid atherosclerosis is a risk factor for ischemic stroke, one of the main causes of mortality and disability worldwide. The disease is characterized by plaques, heterogeneous deposits of lipids, and necrotic debris in the vascular wall, which grow gradually and may remain asymptomatic for decades. However, at some point a plaque can evolve to a high-risk plaque phenotype, which may trigger a cerebrovascular event. Lipids play a key role in the development and progression of atherosclerosis, but the nature of their involvement is not fully understood. Using matrix-assisted laser desorption/ionization mass spectrometry imaging, we visualized the distribution of approximately 200 different lipid signals, originating of >90 uniquely assigned species, in 106 tissue sections of 12 human carotid atherosclerotic plaques. We performed unsupervised classification of the mass spectrometry dataset, as well as a histology-directed multivariate analysis. These data allowed us to extract the spatial lipid patterns associated with morphological plaque features in advanced plaques from a symptomatic population, revealing spatial lipid patterns in atherosclerosis and their relation to histological tissue type. The abundances of sphingomyelin and oxidized cholesteryl ester species were elevated specifically in necrotic intima areas, whereas diacylglycerols and triacylglycerols were spatially correlated to areas containing the coagulation protein fibrin. These results demonstrate a clear colocalization between plaque features and specific lipid classes, as well as individual lipid species in high-risk atherosclerotic plaques. |
format | Online Article Text |
id | pubmed-7881220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-78812202021-03-10 Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging Moerman, Astrid M. Visscher, Mirjam Slijkhuis, Nuria Van Gaalen, Kim Heijs, Bram Klein, Theo Burgers, Peter C. De Rijke, Yolanda B. Van Beusekom, Heleen M.M. Luider, Theo M. Verhagen, Hence J.M. Van der Steen, Antonius F.W. Gijsen, Frank J.H. Van der Heiden, Kim Van Soest, Gijs J Lipid Res Research Article Carotid atherosclerosis is a risk factor for ischemic stroke, one of the main causes of mortality and disability worldwide. The disease is characterized by plaques, heterogeneous deposits of lipids, and necrotic debris in the vascular wall, which grow gradually and may remain asymptomatic for decades. However, at some point a plaque can evolve to a high-risk plaque phenotype, which may trigger a cerebrovascular event. Lipids play a key role in the development and progression of atherosclerosis, but the nature of their involvement is not fully understood. Using matrix-assisted laser desorption/ionization mass spectrometry imaging, we visualized the distribution of approximately 200 different lipid signals, originating of >90 uniquely assigned species, in 106 tissue sections of 12 human carotid atherosclerotic plaques. We performed unsupervised classification of the mass spectrometry dataset, as well as a histology-directed multivariate analysis. These data allowed us to extract the spatial lipid patterns associated with morphological plaque features in advanced plaques from a symptomatic population, revealing spatial lipid patterns in atherosclerosis and their relation to histological tissue type. The abundances of sphingomyelin and oxidized cholesteryl ester species were elevated specifically in necrotic intima areas, whereas diacylglycerols and triacylglycerols were spatially correlated to areas containing the coagulation protein fibrin. These results demonstrate a clear colocalization between plaque features and specific lipid classes, as well as individual lipid species in high-risk atherosclerotic plaques. American Society for Biochemistry and Molecular Biology 2021-01-06 /pmc/articles/PMC7881220/ /pubmed/33581415 http://dx.doi.org/10.1194/jlr.RA120000974 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Moerman, Astrid M. Visscher, Mirjam Slijkhuis, Nuria Van Gaalen, Kim Heijs, Bram Klein, Theo Burgers, Peter C. De Rijke, Yolanda B. Van Beusekom, Heleen M.M. Luider, Theo M. Verhagen, Hence J.M. Van der Steen, Antonius F.W. Gijsen, Frank J.H. Van der Heiden, Kim Van Soest, Gijs Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title_full | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title_fullStr | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title_full_unstemmed | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title_short | Lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
title_sort | lipid signature of advanced human carotid atherosclerosis assessed by mass spectrometry imaging |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881220/ https://www.ncbi.nlm.nih.gov/pubmed/33581415 http://dx.doi.org/10.1194/jlr.RA120000974 |
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