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Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881408/ https://www.ncbi.nlm.nih.gov/pubmed/33585012 |
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author | Safaei, Akram Arefi Oskouie, Afsaneh Mohebbi, Seyed Reza Razaghi, Zahra Nejadi, Naser |
author_facet | Safaei, Akram Arefi Oskouie, Afsaneh Mohebbi, Seyed Reza Razaghi, Zahra Nejadi, Naser |
author_sort | Safaei, Akram |
collection | PubMed |
description | AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis. METHODS: Here, by the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), combined with (MALDI-TOF-TOF MS), proteome profile of decompensated HCV cirrhosis is determined compared to healthy matched controls. Furthermore, Cytoscape has used network analysis. The proteome comparison between two groups identified proteins with significant expression changes (p<0.05 and fold change ≥ 1.5). RESULTS: We found upregulation of IGHA1, C3, A1BG, IGKC and one isoform of HP. Also, lower expression of APOA4 and the other spot of HP in advanced cirrhosis patients were revealed based on HCV compared to matched controls. According to network analysis, ALB has been introduced as a key protein, which may play an important role in pathogenesis. CONCLUSION: Integration of the proteomics with protein interaction data led to the identification of several novel key proteins related to the immune system that may reflect the long-term effects of hepatitis C virus on the liver, and can introduce as therapeutic targets for advanced HCV- cirrhosis. |
format | Online Article Text |
id | pubmed-7881408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-78814082021-02-13 Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers Safaei, Akram Arefi Oskouie, Afsaneh Mohebbi, Seyed Reza Razaghi, Zahra Nejadi, Naser Gastroenterol Hepatol Bed Bench Original Article AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis. METHODS: Here, by the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), combined with (MALDI-TOF-TOF MS), proteome profile of decompensated HCV cirrhosis is determined compared to healthy matched controls. Furthermore, Cytoscape has used network analysis. The proteome comparison between two groups identified proteins with significant expression changes (p<0.05 and fold change ≥ 1.5). RESULTS: We found upregulation of IGHA1, C3, A1BG, IGKC and one isoform of HP. Also, lower expression of APOA4 and the other spot of HP in advanced cirrhosis patients were revealed based on HCV compared to matched controls. According to network analysis, ALB has been introduced as a key protein, which may play an important role in pathogenesis. CONCLUSION: Integration of the proteomics with protein interaction data led to the identification of several novel key proteins related to the immune system that may reflect the long-term effects of hepatitis C virus on the liver, and can introduce as therapeutic targets for advanced HCV- cirrhosis. Shaheed Beheshti University of Medical Sciences 2020 /pmc/articles/PMC7881408/ /pubmed/33585012 Text en ©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Safaei, Akram Arefi Oskouie, Afsaneh Mohebbi, Seyed Reza Razaghi, Zahra Nejadi, Naser Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title | Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title_full | Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title_fullStr | Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title_full_unstemmed | Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title_short | Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers |
title_sort | proteomic study of advanced cirrhosis based on hcv to reveal potential biomarkers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881408/ https://www.ncbi.nlm.nih.gov/pubmed/33585012 |
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