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Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers

AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the...

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Autores principales: Safaei, Akram, Arefi Oskouie, Afsaneh, Mohebbi, Seyed Reza, Razaghi, Zahra, Nejadi, Naser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881408/
https://www.ncbi.nlm.nih.gov/pubmed/33585012
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author Safaei, Akram
Arefi Oskouie, Afsaneh
Mohebbi, Seyed Reza
Razaghi, Zahra
Nejadi, Naser
author_facet Safaei, Akram
Arefi Oskouie, Afsaneh
Mohebbi, Seyed Reza
Razaghi, Zahra
Nejadi, Naser
author_sort Safaei, Akram
collection PubMed
description AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis. METHODS: Here, by the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), combined with (MALDI-TOF-TOF MS), proteome profile of decompensated HCV cirrhosis is determined compared to healthy matched controls. Furthermore, Cytoscape has used network analysis. The proteome comparison between two groups identified proteins with significant expression changes (p<0.05 and fold change ≥ 1.5). RESULTS: We found upregulation of IGHA1, C3, A1BG, IGKC and one isoform of HP. Also, lower expression of APOA4 and the other spot of HP in advanced cirrhosis patients were revealed based on HCV compared to matched controls. According to network analysis, ALB has been introduced as a key protein, which may play an important role in pathogenesis. CONCLUSION: Integration of the proteomics with protein interaction data led to the identification of several novel key proteins related to the immune system that may reflect the long-term effects of hepatitis C virus on the liver, and can introduce as therapeutic targets for advanced HCV- cirrhosis.
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spelling pubmed-78814082021-02-13 Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers Safaei, Akram Arefi Oskouie, Afsaneh Mohebbi, Seyed Reza Razaghi, Zahra Nejadi, Naser Gastroenterol Hepatol Bed Bench Original Article AIM: We aimed to carry out proteomic assessment of long-term effects of hepatitis C on liver. BACKGROUND: Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis Cirrhosis is a condition where liver is damaged and loses its efficiency, and has the high rate of mortality in the world. Proteome profiling may help to identify important proteins and find the pathogenesis. METHODS: Here, by the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), combined with (MALDI-TOF-TOF MS), proteome profile of decompensated HCV cirrhosis is determined compared to healthy matched controls. Furthermore, Cytoscape has used network analysis. The proteome comparison between two groups identified proteins with significant expression changes (p<0.05 and fold change ≥ 1.5). RESULTS: We found upregulation of IGHA1, C3, A1BG, IGKC and one isoform of HP. Also, lower expression of APOA4 and the other spot of HP in advanced cirrhosis patients were revealed based on HCV compared to matched controls. According to network analysis, ALB has been introduced as a key protein, which may play an important role in pathogenesis. CONCLUSION: Integration of the proteomics with protein interaction data led to the identification of several novel key proteins related to the immune system that may reflect the long-term effects of hepatitis C virus on the liver, and can introduce as therapeutic targets for advanced HCV- cirrhosis. Shaheed Beheshti University of Medical Sciences 2020 /pmc/articles/PMC7881408/ /pubmed/33585012 Text en ©2020 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Safaei, Akram
Arefi Oskouie, Afsaneh
Mohebbi, Seyed Reza
Razaghi, Zahra
Nejadi, Naser
Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title_full Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title_fullStr Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title_full_unstemmed Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title_short Proteomic study of advanced cirrhosis based on HCV to reveal potential biomarkers
title_sort proteomic study of advanced cirrhosis based on hcv to reveal potential biomarkers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881408/
https://www.ncbi.nlm.nih.gov/pubmed/33585012
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