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Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts
BACKGROUND: Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. METHODS: The current research was formulated to assess the in-vitro antioxidant and antidi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881459/ https://www.ncbi.nlm.nih.gov/pubmed/33579270 http://dx.doi.org/10.1186/s12906-021-03237-x |
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author | Mustafa, Imtiaz Faisal, Muhammad Naeem Hussain, Ghulam Muzaffar, Humaira Imran, Muhammad Ijaz, Muhammad Umar Sohail, Muhammad Umar Iftikhar, Arslan Shaukat, Arslan Anwar, Haseeb |
author_facet | Mustafa, Imtiaz Faisal, Muhammad Naeem Hussain, Ghulam Muzaffar, Humaira Imran, Muhammad Ijaz, Muhammad Umar Sohail, Muhammad Umar Iftikhar, Arslan Shaukat, Arslan Anwar, Haseeb |
author_sort | Mustafa, Imtiaz |
collection | PubMed |
description | BACKGROUND: Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. METHODS: The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH(●) assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase. RESULTS: After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH(●) (IC(50) value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe(+ 2)/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC(50) value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC(50) value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively). CONCLUSION: The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes. |
format | Online Article Text |
id | pubmed-7881459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78814592021-02-17 Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts Mustafa, Imtiaz Faisal, Muhammad Naeem Hussain, Ghulam Muzaffar, Humaira Imran, Muhammad Ijaz, Muhammad Umar Sohail, Muhammad Umar Iftikhar, Arslan Shaukat, Arslan Anwar, Haseeb BMC Complement Med Ther Research Article BACKGROUND: Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. METHODS: The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH(●) assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase. RESULTS: After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH(●) (IC(50) value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe(+ 2)/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC(50) value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC(50) value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively). CONCLUSION: The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes. BioMed Central 2021-02-12 /pmc/articles/PMC7881459/ /pubmed/33579270 http://dx.doi.org/10.1186/s12906-021-03237-x Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Mustafa, Imtiaz Faisal, Muhammad Naeem Hussain, Ghulam Muzaffar, Humaira Imran, Muhammad Ijaz, Muhammad Umar Sohail, Muhammad Umar Iftikhar, Arslan Shaukat, Arslan Anwar, Haseeb Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title | Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title_full | Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title_fullStr | Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title_full_unstemmed | Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title_short | Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
title_sort | efficacy of euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881459/ https://www.ncbi.nlm.nih.gov/pubmed/33579270 http://dx.doi.org/10.1186/s12906-021-03237-x |
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