One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

BACKGROUND: The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown to increase knock-in efficiency relative to HR,...

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Detalles Bibliográficos
Autores principales: Owen, Joseph R., Hennig, Sadie L., McNabb, Bret R., Mansour, Tamer A., Smith, Justin M., Lin, Jason C., Young, Amy E., Trott, Josephine F., Murray, James D., Delany, Mary E., Ross, Pablo J., Van Eenennaam, Alison L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881600/
https://www.ncbi.nlm.nih.gov/pubmed/33581720
http://dx.doi.org/10.1186/s12864-021-07418-3

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