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KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma

BACKGROUND: In this study, we aimed to explore and clarify the function of KIF4A in esophageal squamous cell carcinoma (ESCC). METHODS: The microarray data were extracted from the Gene Expression Omnibus (GEO) database. We then used the database for Annotation, Visualization, and Integrated Discover...

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Detalles Bibliográficos
Autores principales: Sun, Xiaozheng, Chen, Pengxiang, Chen, Xue, Yang, Wenjing, Chen, Xuan, Zhou, Wei, Huang, Di, Cheng, Yufeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882383/
https://www.ncbi.nlm.nih.gov/pubmed/33350074
http://dx.doi.org/10.1111/1759-7714.13787
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author Sun, Xiaozheng
Chen, Pengxiang
Chen, Xue
Yang, Wenjing
Chen, Xuan
Zhou, Wei
Huang, Di
Cheng, Yufeng
author_facet Sun, Xiaozheng
Chen, Pengxiang
Chen, Xue
Yang, Wenjing
Chen, Xuan
Zhou, Wei
Huang, Di
Cheng, Yufeng
author_sort Sun, Xiaozheng
collection PubMed
description BACKGROUND: In this study, we aimed to explore and clarify the function of KIF4A in esophageal squamous cell carcinoma (ESCC). METHODS: The microarray data were extracted from the Gene Expression Omnibus (GEO) database. We then used the database for Annotation, Visualization, and Integrated Discovery (DAVID) to perform the gene ontology function (GO) and KEGG Orthology‐Based Annotation System (KOBAS) to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs). The six core candidate genes were identified using protein–protein interaction (PPI) network analysis and Cytoscape software. Among them, the expression of KIF4A were validated by UALCAN database from the Cancer Genome Atlas (TCGA) (P < 0.05). Western blotting, qRT‐PCR and IHC were used to detect the expression of KIF4A in tissues. Cell experiments (transwell migration assays, wound healing assay, CCK8 assay, and clone formation experiment) were utilized to verify the roles of KIF4A on the ESCC cells. Western blotting was used to explore the mechanism of KIF4A in ESCC. RESULTS: The expression level of KIF4A was upregulated in ESCC samples compared to those in paracancerous tissues. Transwell migration and wound healing assay showed overexpression of KIF4A significantly promoted the migration of ESCC cells. CCK8 assay and clone formation experiment analysis showed that overexpression of KIF4A promoted proliferation of ESCC cells. Western blot detection found that KIF4A could affect the phosphorylation level of Hippo signaling pathway related proteins. CONCLUSIONS: In summary, KIF4A promotes ESCC cell proliferation and migration by regulating the biological function of ESCC cells through the Hippo signaling pathway. KEY POINTS: SIGNIFICANT FINDINGS OF THE STUDY: We found that high KIF4A expression was associated with poor overall survival in esophageal squamous cell carcinoma. KIF4A expression also promoted the proliferation and migration of ESCC cells in vitro. WHAT THIS STUDY ADDS: Our experimental results explain the role of KIF4A in ESCC, and provide a new biomolecular target for the treatment of ESCC.
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spelling pubmed-78823832021-02-19 KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma Sun, Xiaozheng Chen, Pengxiang Chen, Xue Yang, Wenjing Chen, Xuan Zhou, Wei Huang, Di Cheng, Yufeng Thorac Cancer Original Articles BACKGROUND: In this study, we aimed to explore and clarify the function of KIF4A in esophageal squamous cell carcinoma (ESCC). METHODS: The microarray data were extracted from the Gene Expression Omnibus (GEO) database. We then used the database for Annotation, Visualization, and Integrated Discovery (DAVID) to perform the gene ontology function (GO) and KEGG Orthology‐Based Annotation System (KOBAS) to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs). The six core candidate genes were identified using protein–protein interaction (PPI) network analysis and Cytoscape software. Among them, the expression of KIF4A were validated by UALCAN database from the Cancer Genome Atlas (TCGA) (P < 0.05). Western blotting, qRT‐PCR and IHC were used to detect the expression of KIF4A in tissues. Cell experiments (transwell migration assays, wound healing assay, CCK8 assay, and clone formation experiment) were utilized to verify the roles of KIF4A on the ESCC cells. Western blotting was used to explore the mechanism of KIF4A in ESCC. RESULTS: The expression level of KIF4A was upregulated in ESCC samples compared to those in paracancerous tissues. Transwell migration and wound healing assay showed overexpression of KIF4A significantly promoted the migration of ESCC cells. CCK8 assay and clone formation experiment analysis showed that overexpression of KIF4A promoted proliferation of ESCC cells. Western blot detection found that KIF4A could affect the phosphorylation level of Hippo signaling pathway related proteins. CONCLUSIONS: In summary, KIF4A promotes ESCC cell proliferation and migration by regulating the biological function of ESCC cells through the Hippo signaling pathway. KEY POINTS: SIGNIFICANT FINDINGS OF THE STUDY: We found that high KIF4A expression was associated with poor overall survival in esophageal squamous cell carcinoma. KIF4A expression also promoted the proliferation and migration of ESCC cells in vitro. WHAT THIS STUDY ADDS: Our experimental results explain the role of KIF4A in ESCC, and provide a new biomolecular target for the treatment of ESCC. John Wiley & Sons Australia, Ltd 2020-12-21 2021-02 /pmc/articles/PMC7882383/ /pubmed/33350074 http://dx.doi.org/10.1111/1759-7714.13787 Text en © 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Sun, Xiaozheng
Chen, Pengxiang
Chen, Xue
Yang, Wenjing
Chen, Xuan
Zhou, Wei
Huang, Di
Cheng, Yufeng
KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title_full KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title_fullStr KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title_full_unstemmed KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title_short KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
title_sort kif4a enhanced cell proliferation and migration via hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882383/
https://www.ncbi.nlm.nih.gov/pubmed/33350074
http://dx.doi.org/10.1111/1759-7714.13787
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