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EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis
Circulating tumor cells (CTCs) present an opportunity to detect/monitor metastasis throughout disease progression. The CellSearch® is currently the only FDA-approved technology for CTC detection in patients. The main limitation of this system is its reliance on epithelial markers for CTC isolation/e...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882592/ https://www.ncbi.nlm.nih.gov/pubmed/33415568 http://dx.doi.org/10.1007/s10585-020-10070-y |
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author | Kitz, Jenna Goodale, David Postenka, Carl Lowes, Lori E. Allan, Alison L. |
author_facet | Kitz, Jenna Goodale, David Postenka, Carl Lowes, Lori E. Allan, Alison L. |
author_sort | Kitz, Jenna |
collection | PubMed |
description | Circulating tumor cells (CTCs) present an opportunity to detect/monitor metastasis throughout disease progression. The CellSearch® is currently the only FDA-approved technology for CTC detection in patients. The main limitation of this system is its reliance on epithelial markers for CTC isolation/enumeration, which reduces its ability to detect more aggressive mesenchymal CTCs that are generated during metastasis via epithelial-to-mesenchymal transition (EMT). This Technical Note describes and validates two EMT-independent CTC analysis protocols; one for human samples using Parsortix® and one for mouse samples using VyCap. Parsortix® identifies significantly more mesenchymal human CTCs compared to the clinical CellSearch® test, and VyCap identifies significantly more CTCs compared to our mouse CellSearch® protocol regardless of EMT status. Recovery and downstream molecular characterization of CTCs is highly feasible using both Parsortix® and VyCap. The described CTC protocols can be used by investigators to study CTC generation, EMT and metastasis in both pre-clinical models and clinical samples. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10585-020-10070-y. |
format | Online Article Text |
id | pubmed-7882592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-78825922021-02-25 EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis Kitz, Jenna Goodale, David Postenka, Carl Lowes, Lori E. Allan, Alison L. Clin Exp Metastasis Technical Note Circulating tumor cells (CTCs) present an opportunity to detect/monitor metastasis throughout disease progression. The CellSearch® is currently the only FDA-approved technology for CTC detection in patients. The main limitation of this system is its reliance on epithelial markers for CTC isolation/enumeration, which reduces its ability to detect more aggressive mesenchymal CTCs that are generated during metastasis via epithelial-to-mesenchymal transition (EMT). This Technical Note describes and validates two EMT-independent CTC analysis protocols; one for human samples using Parsortix® and one for mouse samples using VyCap. Parsortix® identifies significantly more mesenchymal human CTCs compared to the clinical CellSearch® test, and VyCap identifies significantly more CTCs compared to our mouse CellSearch® protocol regardless of EMT status. Recovery and downstream molecular characterization of CTCs is highly feasible using both Parsortix® and VyCap. The described CTC protocols can be used by investigators to study CTC generation, EMT and metastasis in both pre-clinical models and clinical samples. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10585-020-10070-y. Springer Netherlands 2021-01-07 2021 /pmc/articles/PMC7882592/ /pubmed/33415568 http://dx.doi.org/10.1007/s10585-020-10070-y Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Technical Note Kitz, Jenna Goodale, David Postenka, Carl Lowes, Lori E. Allan, Alison L. EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title | EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title_full | EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title_fullStr | EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title_full_unstemmed | EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title_short | EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
title_sort | emt-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis |
topic | Technical Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882592/ https://www.ncbi.nlm.nih.gov/pubmed/33415568 http://dx.doi.org/10.1007/s10585-020-10070-y |
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