Cargando…

WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps

Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, b...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Yun, Tian, Peng, Zhong, Hua, Wu, Fan, Zhang, Qining, Liu, Xiang, Dang, Hua, Chen, Qiujian, Zou, Hua, Zheng, Yiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882705/
https://www.ncbi.nlm.nih.gov/pubmed/33598458
http://dx.doi.org/10.3389/fcell.2020.630340
_version_ 1783651101100212224
author Ma, Yun
Tian, Peng
Zhong, Hua
Wu, Fan
Zhang, Qining
Liu, Xiang
Dang, Hua
Chen, Qiujian
Zou, Hua
Zheng, Yiqing
author_facet Ma, Yun
Tian, Peng
Zhong, Hua
Wu, Fan
Zhang, Qining
Liu, Xiang
Dang, Hua
Chen, Qiujian
Zou, Hua
Zheng, Yiqing
author_sort Ma, Yun
collection PubMed
description Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, but its role in the beating of cilia remains unclear. In this study, we sought to investigate the role of WDPCP and its underlying mechanism behind the dysfunction in the beating of cilia in nasal polyp tissue. We demonstrated WDPCP expression in the epithelium of nasal polyps. We also investigated the MAPK/ERK pathway in primary human sinonasal epithelial cells to explore the function of WDPCP. The air–liquid interface culture system was used as a model to verify the role of WDPCP and the MAPK/ERK pathway in the beating of cilia. With the dysfunction of cilia beating, we observed a low expression of WDPCP in the epithelium of nasal polyp tissues. Within the in vitro study, we found that WDPCP was critical for mitochondrial biogenesis and mitochondrial function in human sinonasal epithelial cells, possibly due to the activation of the MAPK/ERK pathway. The mitochondrial dysfunction caused by U0126 or lacking WDPCP could be partially recovered by dexamethasone. The low expression of WDPCP in nasal epithelium could affect mitochondria via the MAPK/ERK pathway, which may contribute to the dysfunction in the beating of cilia in CRSwNP.
format Online
Article
Text
id pubmed-7882705
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78827052021-02-16 WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps Ma, Yun Tian, Peng Zhong, Hua Wu, Fan Zhang, Qining Liu, Xiang Dang, Hua Chen, Qiujian Zou, Hua Zheng, Yiqing Front Cell Dev Biol Cell and Developmental Biology Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, but its role in the beating of cilia remains unclear. In this study, we sought to investigate the role of WDPCP and its underlying mechanism behind the dysfunction in the beating of cilia in nasal polyp tissue. We demonstrated WDPCP expression in the epithelium of nasal polyps. We also investigated the MAPK/ERK pathway in primary human sinonasal epithelial cells to explore the function of WDPCP. The air–liquid interface culture system was used as a model to verify the role of WDPCP and the MAPK/ERK pathway in the beating of cilia. With the dysfunction of cilia beating, we observed a low expression of WDPCP in the epithelium of nasal polyp tissues. Within the in vitro study, we found that WDPCP was critical for mitochondrial biogenesis and mitochondrial function in human sinonasal epithelial cells, possibly due to the activation of the MAPK/ERK pathway. The mitochondrial dysfunction caused by U0126 or lacking WDPCP could be partially recovered by dexamethasone. The low expression of WDPCP in nasal epithelium could affect mitochondria via the MAPK/ERK pathway, which may contribute to the dysfunction in the beating of cilia in CRSwNP. Frontiers Media S.A. 2021-02-01 /pmc/articles/PMC7882705/ /pubmed/33598458 http://dx.doi.org/10.3389/fcell.2020.630340 Text en Copyright © 2021 Ma, Tian, Zhong, Wu, Zhang, Liu, Dang, Chen, Zou and Zheng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ma, Yun
Tian, Peng
Zhong, Hua
Wu, Fan
Zhang, Qining
Liu, Xiang
Dang, Hua
Chen, Qiujian
Zou, Hua
Zheng, Yiqing
WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title_full WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title_fullStr WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title_full_unstemmed WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title_short WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
title_sort wdpcp modulates cilia beating through the mapk/erk pathway in chronic rhinosinusitis with nasal polyps
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882705/
https://www.ncbi.nlm.nih.gov/pubmed/33598458
http://dx.doi.org/10.3389/fcell.2020.630340
work_keys_str_mv AT mayun wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT tianpeng wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT zhonghua wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT wufan wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT zhangqining wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT liuxiang wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT danghua wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT chenqiujian wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT zouhua wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps
AT zhengyiqing wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps