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Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs

Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique....

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Autores principales: Zhang, Jing, Xu, Xin, Chen, Hongbo, Kang, Ping, Zhu, Huiling, Ren, Hongyan, Liu, Yulan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882806/
https://www.ncbi.nlm.nih.gov/pubmed/33504244
http://dx.doi.org/10.1177/1753425920983869
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author Zhang, Jing
Xu, Xin
Chen, Hongbo
Kang, Ping
Zhu, Huiling
Ren, Hongyan
Liu, Yulan
author_facet Zhang, Jing
Xu, Xin
Chen, Hongbo
Kang, Ping
Zhu, Huiling
Ren, Hongyan
Liu, Yulan
author_sort Zhang, Jing
collection PubMed
description Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique. In total 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated these genes were involved in inflammation-related signaling pathways, including cytokine–cytokine receptor interaction, TNF-α, NF-κB, Jak-STAT and TLR signaling pathways. In addition, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expressions of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) were validated in the LPS-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that expression of three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) was significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which indicated that these lncRNAs might be important regulators for inflammation. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs, which might provide potential insights into lncRNAs involved in regulating inflammation in pigs.
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spelling pubmed-78828062021-02-23 Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs Zhang, Jing Xu, Xin Chen, Hongbo Kang, Ping Zhu, Huiling Ren, Hongyan Liu, Yulan Innate Immun Original Articles Long non-coding RNAs (lncRNAs) are emerging as key regulators in inflammation. However, their functions and profiles in LPS-induced inflammation in pigs are largely unknown. In this study, we profiled global lncRNA and mRNA expression changes in PBMCs treated with LPS using the lncRNA-seq technique. In total 43 differentially expressed (DE) lncRNAs and 1082 DE mRNAs were identified in porcine PBMCs after LPS stimulation. Functional enrichment analysis on DE mRNAs indicated these genes were involved in inflammation-related signaling pathways, including cytokine–cytokine receptor interaction, TNF-α, NF-κB, Jak-STAT and TLR signaling pathways. In addition, co-expression network and function analysis identified the potential lncRNAs related to inflammatory response and immune response. The expressions of eight lncRNAs (ENSSSCT00000045208, ENSSSCT00000051636, ENSSSCT00000049770, ENSSSCT00000050966, ENSSSCT00000047491, ENSSSCT00000049750, ENSSSCT00000054262 and ENSSSCT00000044651) were validated in the LPS-treated PBMCs by quantitative real-time PCR (qRT-PCR). In LPS-challenged piglets, we identified that expression of three lncRNAs (ENSSSCT00000051636, ENSSSCT00000049770, and ENSSSCT00000047491) was significantly up-regulated in liver, spleen and jejunum tissues after LPS challenge, which indicated that these lncRNAs might be important regulators for inflammation. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS-mediated changes in porcine PBMCs, which might provide potential insights into lncRNAs involved in regulating inflammation in pigs. SAGE Publications 2021-01-27 2021-02 /pmc/articles/PMC7882806/ /pubmed/33504244 http://dx.doi.org/10.1177/1753425920983869 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Zhang, Jing
Xu, Xin
Chen, Hongbo
Kang, Ping
Zhu, Huiling
Ren, Hongyan
Liu, Yulan
Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title_full Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title_fullStr Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title_full_unstemmed Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title_short Construction and analysis for dys-regulated lncRNAs and mRNAs in LPS-induced porcine PBMCs
title_sort construction and analysis for dys-regulated lncrnas and mrnas in lps-induced porcine pbmcs
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882806/
https://www.ncbi.nlm.nih.gov/pubmed/33504244
http://dx.doi.org/10.1177/1753425920983869
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