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Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases

Point-of-care (POC) molecular diagnostics for clinical microbiology and virology has primarily focused on the detection of a single pathogen. More recently, it has transitioned into a comprehensive syndromic approach that employs multiplex capabilities, including the simultaneous detection of two or...

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Autores principales: Kim, Hanbi, Huh, Hee Jae, Park, Eunkyoung, Chung, Doo-Ryeon, Kang, Minhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean BioChip Society (KBCS) 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883532/
https://www.ncbi.nlm.nih.gov/pubmed/33613852
http://dx.doi.org/10.1007/s13206-021-00004-5
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author Kim, Hanbi
Huh, Hee Jae
Park, Eunkyoung
Chung, Doo-Ryeon
Kang, Minhee
author_facet Kim, Hanbi
Huh, Hee Jae
Park, Eunkyoung
Chung, Doo-Ryeon
Kang, Minhee
author_sort Kim, Hanbi
collection PubMed
description Point-of-care (POC) molecular diagnostics for clinical microbiology and virology has primarily focused on the detection of a single pathogen. More recently, it has transitioned into a comprehensive syndromic approach that employs multiplex capabilities, including the simultaneous detection of two or more pathogens. Multiplex POC tests provide higher accuracy to for actionable decisionmaking in critical care, which leads to pathogen-specific treatment and standardized usages of antibiotics that help prevent unnecessary processes. In addition, these tests can be simple enough to operate at the primary care level and in remote settings where there is no laboratory infrastructure. This review focuses on state-of-the-art multiplexed molecular point-of-care tests (POCT) for infectious diseases and efforts to overcome their limitations, especially related to inadequate throughput for the identification of syndromic diseases. We also discuss promising and imperative clinical POC approaches, as well as the possible hurdles of their practical applications as front-line diagnostic tests.
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spelling pubmed-78835322021-02-16 Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases Kim, Hanbi Huh, Hee Jae Park, Eunkyoung Chung, Doo-Ryeon Kang, Minhee Biochip J Review Article Point-of-care (POC) molecular diagnostics for clinical microbiology and virology has primarily focused on the detection of a single pathogen. More recently, it has transitioned into a comprehensive syndromic approach that employs multiplex capabilities, including the simultaneous detection of two or more pathogens. Multiplex POC tests provide higher accuracy to for actionable decisionmaking in critical care, which leads to pathogen-specific treatment and standardized usages of antibiotics that help prevent unnecessary processes. In addition, these tests can be simple enough to operate at the primary care level and in remote settings where there is no laboratory infrastructure. This review focuses on state-of-the-art multiplexed molecular point-of-care tests (POCT) for infectious diseases and efforts to overcome their limitations, especially related to inadequate throughput for the identification of syndromic diseases. We also discuss promising and imperative clinical POC approaches, as well as the possible hurdles of their practical applications as front-line diagnostic tests. The Korean BioChip Society (KBCS) 2021-02-15 2021 /pmc/articles/PMC7883532/ /pubmed/33613852 http://dx.doi.org/10.1007/s13206-021-00004-5 Text en © The Korean BioChip Society 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Article
Kim, Hanbi
Huh, Hee Jae
Park, Eunkyoung
Chung, Doo-Ryeon
Kang, Minhee
Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title_full Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title_fullStr Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title_full_unstemmed Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title_short Multiplex Molecular Point-of-Care Test for Syndromic Infectious Diseases
title_sort multiplex molecular point-of-care test for syndromic infectious diseases
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883532/
https://www.ncbi.nlm.nih.gov/pubmed/33613852
http://dx.doi.org/10.1007/s13206-021-00004-5
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