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Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet

The freshwater pulmonate snail Planorbella trivolvis is a common species in various bodies of water but is not native to China. Planorbella trivolvis usually live on diets with high fiber content, such as water grasses, algae and fallen leaves. These snails can attach to the wall of a water tank or...

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Autores principales: Hu, Zongfu, Tong, Qing, Chang, Jie, Yu, Jianhua, Li, Shuguo, Niu, Huaxin, Ma, Deying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883694/
https://www.ncbi.nlm.nih.gov/pubmed/33614273
http://dx.doi.org/10.7717/peerj.10716
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author Hu, Zongfu
Tong, Qing
Chang, Jie
Yu, Jianhua
Li, Shuguo
Niu, Huaxin
Ma, Deying
author_facet Hu, Zongfu
Tong, Qing
Chang, Jie
Yu, Jianhua
Li, Shuguo
Niu, Huaxin
Ma, Deying
author_sort Hu, Zongfu
collection PubMed
description The freshwater pulmonate snail Planorbella trivolvis is a common species in various bodies of water but is not native to China. Planorbella trivolvis usually live on diets with high fiber content, such as water grasses, algae and fallen leaves. These snails can attach to the wall of a water tank or to water grass and can be transported overseas to China through the ornamental fish trade. There are few studies investigating the intestinal microbiota of freshwater snails. In this study, using culture-independent molecular analysis, we assessed for the first time the complexity of bacterial communities in the intestines of reared snails. The intestinal microbiota in the snails fed different diets, that is, herbivorous feed (HV) with high cellulose and non-herbivorous feed (NHV) with low cellulose, were analyzed by Illumina sequencing. The results showed that the NHV-based diet significantly increased the body mass, shell diameter and specific growth rate of the snails after 60 days of rearing (P < 0.05). Histological experiments showed that the fat droplets in the epithelium columnar cells of the intestines of the NHV snails increased, and the cilia on these cells fell off. The sequencing results identified 486 and 195 OTUs in HV and NHV, respectively. Lots of bacteria were not reported previously in snails. The intestinal microbiota diversity index (Shannon, Simpson, Ace and Chao) in the NHV snails was significantly lower than that in the HV snails. The gut microbiota in the HV snails were predominantly Proteobacteria (52.97%) and Bacteroidetes (28.75%), while the gut microbiota in NHV snails were predominantly Proteobacteria (95.23%). At the genus level, Cloacibacterium (24.60%), Pseudomonas (4.47%), OM6ON (6.12%), and Rhodobacter (5.79%) were observed to be abundant in HV snails. However, Aeromonas (85.4%) was determined to be predominant in NHV snails. Functional prediction of the gut microbiome in snails by PICRUSt demonstrated a significant difference between the two groups, and the HV snails exhibited higher lignocellulose enzyme activity than did the NHV snails. This study represents a first step in characterizing the gut microbiota of the freshwater snail. Most of these microbes can process plant biomass and digest cellulose and lignocellulose, and the enzymes of these bacteria may have potential biotechnological applications in a variety of industrial processes.
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spelling pubmed-78836942021-02-19 Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet Hu, Zongfu Tong, Qing Chang, Jie Yu, Jianhua Li, Shuguo Niu, Huaxin Ma, Deying PeerJ Aquaculture, Fisheries and Fish Science The freshwater pulmonate snail Planorbella trivolvis is a common species in various bodies of water but is not native to China. Planorbella trivolvis usually live on diets with high fiber content, such as water grasses, algae and fallen leaves. These snails can attach to the wall of a water tank or to water grass and can be transported overseas to China through the ornamental fish trade. There are few studies investigating the intestinal microbiota of freshwater snails. In this study, using culture-independent molecular analysis, we assessed for the first time the complexity of bacterial communities in the intestines of reared snails. The intestinal microbiota in the snails fed different diets, that is, herbivorous feed (HV) with high cellulose and non-herbivorous feed (NHV) with low cellulose, were analyzed by Illumina sequencing. The results showed that the NHV-based diet significantly increased the body mass, shell diameter and specific growth rate of the snails after 60 days of rearing (P < 0.05). Histological experiments showed that the fat droplets in the epithelium columnar cells of the intestines of the NHV snails increased, and the cilia on these cells fell off. The sequencing results identified 486 and 195 OTUs in HV and NHV, respectively. Lots of bacteria were not reported previously in snails. The intestinal microbiota diversity index (Shannon, Simpson, Ace and Chao) in the NHV snails was significantly lower than that in the HV snails. The gut microbiota in the HV snails were predominantly Proteobacteria (52.97%) and Bacteroidetes (28.75%), while the gut microbiota in NHV snails were predominantly Proteobacteria (95.23%). At the genus level, Cloacibacterium (24.60%), Pseudomonas (4.47%), OM6ON (6.12%), and Rhodobacter (5.79%) were observed to be abundant in HV snails. However, Aeromonas (85.4%) was determined to be predominant in NHV snails. Functional prediction of the gut microbiome in snails by PICRUSt demonstrated a significant difference between the two groups, and the HV snails exhibited higher lignocellulose enzyme activity than did the NHV snails. This study represents a first step in characterizing the gut microbiota of the freshwater snail. Most of these microbes can process plant biomass and digest cellulose and lignocellulose, and the enzymes of these bacteria may have potential biotechnological applications in a variety of industrial processes. PeerJ Inc. 2021-02-12 /pmc/articles/PMC7883694/ /pubmed/33614273 http://dx.doi.org/10.7717/peerj.10716 Text en © 2021 Hu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
Hu, Zongfu
Tong, Qing
Chang, Jie
Yu, Jianhua
Li, Shuguo
Niu, Huaxin
Ma, Deying
Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title_full Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title_fullStr Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title_full_unstemmed Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title_short Gut bacterial communities in the freshwater snail Planorbella trivolvis and their modification by a non-herbivorous diet
title_sort gut bacterial communities in the freshwater snail planorbella trivolvis and their modification by a non-herbivorous diet
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883694/
https://www.ncbi.nlm.nih.gov/pubmed/33614273
http://dx.doi.org/10.7717/peerj.10716
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