High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells

The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conju...

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Autores principales: Ma, Ruiqi, Gan, Lu, Jiang, Sanjie, Ding, Peiwen, Chen, Dongsheng, Wu, Jihong, Qian, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883706/
https://www.ncbi.nlm.nih.gov/pubmed/33600811
http://dx.doi.org/10.1016/j.exer.2021.108501
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author Ma, Ruiqi
Gan, Lu
Jiang, Sanjie
Ding, Peiwen
Chen, Dongsheng
Wu, Jihong
Qian, Jiang
author_facet Ma, Ruiqi
Gan, Lu
Jiang, Sanjie
Ding, Peiwen
Chen, Dongsheng
Wu, Jihong
Qian, Jiang
author_sort Ma, Ruiqi
collection PubMed
description The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection.
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spelling pubmed-78837062021-02-16 High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells Ma, Ruiqi Gan, Lu Jiang, Sanjie Ding, Peiwen Chen, Dongsheng Wu, Jihong Qian, Jiang Exp Eye Res Article The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TMPRSS2 were highly co-expressed in the goblet cells with genes involved in immunity process. This identification of conjunctival cell types which are permissive to virus entry would help to understand the process by which SARS-CoV-2 infection was established. These finding might be suggestive for COVID-19 control and protection. The Authors. Published by Elsevier Ltd. 2021-04 2021-02-15 /pmc/articles/PMC7883706/ /pubmed/33600811 http://dx.doi.org/10.1016/j.exer.2021.108501 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ma, Ruiqi
Gan, Lu
Jiang, Sanjie
Ding, Peiwen
Chen, Dongsheng
Wu, Jihong
Qian, Jiang
High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title_full High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title_fullStr High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title_full_unstemmed High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title_short High expression of SARS-CoV-2 entry factors in human conjunctival goblet cells
title_sort high expression of sars-cov-2 entry factors in human conjunctival goblet cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883706/
https://www.ncbi.nlm.nih.gov/pubmed/33600811
http://dx.doi.org/10.1016/j.exer.2021.108501
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