RNF219 interacts with CCR4–NOT in regulating stem cell differentiation

Regulation of RNA stability plays a crucial role in gene expression control. Deadenylation is the initial rate-limiting step for the majority of RNA decay events. Here, we show that RING finger protein 219 (RNF219) interacts with the CCR4–NOT deadenylase complex. RNF219–CCR4–NOT exhibits deadenylati...

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Autores principales: Du, Hao, Chen, Chen, Wang, Yan, Yang, Yang, Che, Zhuanzhuan, Liu, Xiaoxu, Meng, Siyan, Guo, Chenghao, Xu, Manman, Fang, Haitong, Wang, Fengchao, Lin, Chengqi, Luo, Zhuojuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883825/
https://www.ncbi.nlm.nih.gov/pubmed/33104214
http://dx.doi.org/10.1093/jmcb/mjaa061
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author Du, Hao
Chen, Chen
Wang, Yan
Yang, Yang
Che, Zhuanzhuan
Liu, Xiaoxu
Meng, Siyan
Guo, Chenghao
Xu, Manman
Fang, Haitong
Wang, Fengchao
Lin, Chengqi
Luo, Zhuojuan
author_facet Du, Hao
Chen, Chen
Wang, Yan
Yang, Yang
Che, Zhuanzhuan
Liu, Xiaoxu
Meng, Siyan
Guo, Chenghao
Xu, Manman
Fang, Haitong
Wang, Fengchao
Lin, Chengqi
Luo, Zhuojuan
author_sort Du, Hao
collection PubMed
description Regulation of RNA stability plays a crucial role in gene expression control. Deadenylation is the initial rate-limiting step for the majority of RNA decay events. Here, we show that RING finger protein 219 (RNF219) interacts with the CCR4–NOT deadenylase complex. RNF219–CCR4–NOT exhibits deadenylation activity in vitro. RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown, while upregulated after depletion of the CCR4–NOT subunit CNOT10 in mouse embryonic stem (ES) cells. RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation. Our study suggests that RNF219 is a novel interacting partner of CCR4–NOT and required for maintenance of ES cell pluripotency.
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spelling pubmed-78838252021-02-18 RNF219 interacts with CCR4–NOT in regulating stem cell differentiation Du, Hao Chen, Chen Wang, Yan Yang, Yang Che, Zhuanzhuan Liu, Xiaoxu Meng, Siyan Guo, Chenghao Xu, Manman Fang, Haitong Wang, Fengchao Lin, Chengqi Luo, Zhuojuan J Mol Cell Biol Articles Regulation of RNA stability plays a crucial role in gene expression control. Deadenylation is the initial rate-limiting step for the majority of RNA decay events. Here, we show that RING finger protein 219 (RNF219) interacts with the CCR4–NOT deadenylase complex. RNF219–CCR4–NOT exhibits deadenylation activity in vitro. RNA-seq analyses identify some of the 2-cell-specific genes and the neuronal genes significantly downregulated upon RNF219 knockdown, while upregulated after depletion of the CCR4–NOT subunit CNOT10 in mouse embryonic stem (ES) cells. RNF219 depletion leads to impaired neuronal lineage commitment during ES cell differentiation. Our study suggests that RNF219 is a novel interacting partner of CCR4–NOT and required for maintenance of ES cell pluripotency. Oxford University Press 2020-10-26 /pmc/articles/PMC7883825/ /pubmed/33104214 http://dx.doi.org/10.1093/jmcb/mjaa061 Text en © The Author(s) (2020). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Du, Hao
Chen, Chen
Wang, Yan
Yang, Yang
Che, Zhuanzhuan
Liu, Xiaoxu
Meng, Siyan
Guo, Chenghao
Xu, Manman
Fang, Haitong
Wang, Fengchao
Lin, Chengqi
Luo, Zhuojuan
RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title_full RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title_fullStr RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title_full_unstemmed RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title_short RNF219 interacts with CCR4–NOT in regulating stem cell differentiation
title_sort rnf219 interacts with ccr4–not in regulating stem cell differentiation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883825/
https://www.ncbi.nlm.nih.gov/pubmed/33104214
http://dx.doi.org/10.1093/jmcb/mjaa061
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