Cargando…

Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone

Echinops kebericho Mesfin is traditionally used for the treatment of various infectious diseases. This study investigated antibacterial activity of the essential oil (EO) and the different fractions of ethanol extract. The most active component was isolated and identified. Isolation and purification...

Descripción completa

Detalles Bibliográficos
Autores principales: Deyno, Serawit, Mtewa, Andrew G., Hope, Derick, Bazira, Joel, Makonnen, Eyasu, Alele, Paul E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883827/
https://www.ncbi.nlm.nih.gov/pubmed/33597879
http://dx.doi.org/10.3389/fphar.2020.608672
_version_ 1783651291563556864
author Deyno, Serawit
Mtewa, Andrew G.
Hope, Derick
Bazira, Joel
Makonnen, Eyasu
Alele, Paul E.
author_facet Deyno, Serawit
Mtewa, Andrew G.
Hope, Derick
Bazira, Joel
Makonnen, Eyasu
Alele, Paul E.
author_sort Deyno, Serawit
collection PubMed
description Echinops kebericho Mesfin is traditionally used for the treatment of various infectious diseases. This study investigated antibacterial activity of the essential oil (EO) and the different fractions of ethanol extract. The most active component was isolated and identified. Isolation and purification was accomplished using chromatographic techniques while identification was done by spectroscopic method. Minimum inhibitory concentration (MIC) was determined using the broth micro-dilution method. In bioactive-guided isolation, percent inhibition was determined using optical density (OD) measurement. The MICs of the essential oil ranged from 78.125 μg/ml to 625 μg/ml, and its activity was observed against methicillin-resistant Staphylococcus aureus (MRSA, NCTC 12493). Ethyl acetate fraction showed high activity against MRSA (NCTC 12493), MIC = 39.075 μg/ml followed by Enterococcus faecalis (ATCC 49532), MIC = 78.125 μg/ml and was least active against Klebsiella pneumoniae (ATCC 700603), MIC = 1,250 μg/ml. MIC of hexane fraction ranged from 156.2 µg/ml to Escherichia coli (ATCC 49532) to 1,250 μg/ml to E. coli (NCTC 11954). The MICs of chloroform fraction ranged from 312.5 to 2500 μg/ml; while butanol fraction could be considered pharmacologically inactive as its MIC value was 2,500 μg/ml for all and no activity against E. coli (NCTC 11954). Dehydrocostus lactone was successfully isolated and identified whose MIC was 19.53 μg/ml against MRSA. Dehydrocostus lactone isolated from E. kebericho M. showed noteworthy antibacterial activity which lends support to ethnopharmacological use of the plant. Further optimization should be done to improve its antibacterial activities and pharmacokinetic profile.
format Online
Article
Text
id pubmed-7883827
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78838272021-02-16 Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone Deyno, Serawit Mtewa, Andrew G. Hope, Derick Bazira, Joel Makonnen, Eyasu Alele, Paul E. Front Pharmacol Pharmacology Echinops kebericho Mesfin is traditionally used for the treatment of various infectious diseases. This study investigated antibacterial activity of the essential oil (EO) and the different fractions of ethanol extract. The most active component was isolated and identified. Isolation and purification was accomplished using chromatographic techniques while identification was done by spectroscopic method. Minimum inhibitory concentration (MIC) was determined using the broth micro-dilution method. In bioactive-guided isolation, percent inhibition was determined using optical density (OD) measurement. The MICs of the essential oil ranged from 78.125 μg/ml to 625 μg/ml, and its activity was observed against methicillin-resistant Staphylococcus aureus (MRSA, NCTC 12493). Ethyl acetate fraction showed high activity against MRSA (NCTC 12493), MIC = 39.075 μg/ml followed by Enterococcus faecalis (ATCC 49532), MIC = 78.125 μg/ml and was least active against Klebsiella pneumoniae (ATCC 700603), MIC = 1,250 μg/ml. MIC of hexane fraction ranged from 156.2 µg/ml to Escherichia coli (ATCC 49532) to 1,250 μg/ml to E. coli (NCTC 11954). The MICs of chloroform fraction ranged from 312.5 to 2500 μg/ml; while butanol fraction could be considered pharmacologically inactive as its MIC value was 2,500 μg/ml for all and no activity against E. coli (NCTC 11954). Dehydrocostus lactone was successfully isolated and identified whose MIC was 19.53 μg/ml against MRSA. Dehydrocostus lactone isolated from E. kebericho M. showed noteworthy antibacterial activity which lends support to ethnopharmacological use of the plant. Further optimization should be done to improve its antibacterial activities and pharmacokinetic profile. Frontiers Media S.A. 2021-01-25 /pmc/articles/PMC7883827/ /pubmed/33597879 http://dx.doi.org/10.3389/fphar.2020.608672 Text en Copyright © 2021 Deyno, Mtewa, Hope, Bazira, Makonnen and Alele. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Deyno, Serawit
Mtewa, Andrew G.
Hope, Derick
Bazira, Joel
Makonnen, Eyasu
Alele, Paul E.
Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title_full Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title_fullStr Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title_full_unstemmed Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title_short Antibacterial Activities of Echinops kebericho Mesfin Tuber Extracts and Isolation of the Most Active Compound, Dehydrocostus Lactone
title_sort antibacterial activities of echinops kebericho mesfin tuber extracts and isolation of the most active compound, dehydrocostus lactone
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883827/
https://www.ncbi.nlm.nih.gov/pubmed/33597879
http://dx.doi.org/10.3389/fphar.2020.608672
work_keys_str_mv AT deynoserawit antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone
AT mtewaandrewg antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone
AT hopederick antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone
AT bazirajoel antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone
AT makonneneyasu antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone
AT alelepaule antibacterialactivitiesofechinopskeberichomesfintuberextractsandisolationofthemostactivecompounddehydrocostuslactone